Restriction map for
pFN21AE6415
(Linear) (Six-base) MAPSORT of: KIEE6415.seq Check: 2374 from: 1 to: 1440
KIEE6415.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AatII G_ACGT'C
Cuts at: 0 312 1440
Size: 312 1128
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1440
Size: 281 1159
AleI CACnn'nnGTG
Cuts at: 0 815 1440
Size: 815 625
ApaLI G'TGCA_C
Cuts at: 0 653 1440
Size: 653 787
ApoI r'AATT_y
Cuts at: 0 467 1157 1375 1440
Size: 467 690 218 65
Fragments arranged by size:
690 467 218 65
BanI G'GyrC_C
Cuts at: 0 1030 1440
Size: 1030 410
BanII G_rGCy'C
Cuts at: 0 672 1088 1330 1440
Size: 672 416 242 110
Fragments arranged by size:
672 416 242 110
BbeI G_GCGC'C
Cuts at: 0 1034 1440
Size: 1034 406
BbsI GAAGACnn'nnnn_
Cuts at: 0 1259 1440
Size: 1259 181
BbvCI CC'TCA_GC
Cuts at: 0 1025 1440
Size: 1025 415
BglI GCCn_nnn'nGGC
Cuts at: 0 58 256 1440
Size: 58 198 1184
Fragments arranged by size:
1184 198 58
BglII A'GATC_T
Cuts at: 0 116 995 1136 1440
Size: 116 879 141 304
Fragments arranged by size:
879 304 141 116
Bme1580I G_kGCm'C
Cuts at: 0 657 1440
Size: 657 783
BmrI ACTGGGnnnn_n'
Cuts at: 0 1069 1440
Size: 1069 371
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 752 1440
Size: 752 688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 1440
Size: 578 862
Bpu10I CC'TnA_GC
Cuts at: 0 1025 1440
Size: 1025 415
BsaHI Gr'CG_yC
Cuts at: 0 61 309 425 1031 1440
Size: 61 248 116 606 409
Fragments arranged by size:
606 409 248 116 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 179 209 1440
Size: 179 30 1231
Fragments arranged by size:
1231 179 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 886 901 1147 1440
Size: 886 15 246 293
Fragments arranged by size:
886 293 246 15
BseYI C'CCAG_C
Cuts at: 0 455 1282 1440
Size: 455 827 158
Fragments arranged by size:
827 455 158
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 262 828 1440
Size: 52 210 566 612
Fragments arranged by size:
612 566 210 52
BsiHKAI G_wGCw'C
Cuts at: 0 657 1440
Size: 657 783
Bsp1286I G_dGCh'C
Cuts at: 0 657 672 1088 1330 1440
Size: 657 15 416 242 110
Fragments arranged by size:
657 416 242 110 15
BsrBI CCG'CTC
Cuts at: 0 912 1440
Size: 912 528
BsrDI GCAATG_nn'
Cuts at: 0 1280 1440
Size: 1280 160
BstAPI GCAn_nnn'nTGC
Cuts at: 0 75 358 1440
Size: 75 283 1082
Fragments arranged by size:
1082 283 75
BstYI r'GATC_y
Cuts at: 0 116 995 1136 1421 1440
Size: 116 879 141 285 19
Fragments arranged by size:
879 285 141 116 19
BtgI C'CryG_G
Cuts at: 0 365 553 1440
Size: 365 188 887
Fragments arranged by size:
887 365 188
BtsI GCAGTG_nn'
Cuts at: 0 245 845 1440
Size: 245 600 595
Fragments arranged by size:
600 595 245
DraIII CAC_nnn'GTG
Cuts at: 0 652 1440
Size: 652 788
EarI CTCTTCn'nnn_
Cuts at: 0 721 1440
Size: 721 719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 578 1440
Size: 281 297 862
Fragments arranged by size:
862 297 281
EcoNI CCTnn'n_nnAGG
Cuts at: 0 676 1440
Size: 676 764
EcoO109I rG'GnC_Cy
Cuts at: 0 964 1254 1440
Size: 964 290 186
Fragments arranged by size:
964 290 186
HaeII r_GCGC'y
Cuts at: 0 1034 1440
Size: 1034 406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 533 565 659 691 1440
Size: 533 32 94 32 749
Fragments arranged by size:
749 533 94 32 32
HindIII A'AGCT_T
Cuts at: 0 322 401 691 1440
Size: 322 79 290 749
Fragments arranged by size:
749 322 290 79
KasI G'GCGC_C
Cuts at: 0 1030 1440
Size: 1030 410
MfeI C'AATT_G
Cuts at: 0 1299 1440
Size: 1299 141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 666 1124 1440
Size: 296 370 458 316
Fragments arranged by size:
458 370 316 296
MslI CAynn'nnrTG
Cuts at: 0 532 815 865 1440
Size: 532 283 50 575
Fragments arranged by size:
575 532 283 50
MspA1I CmG'CkG
Cuts at: 0 709 1228 1440
Size: 709 519 212
Fragments arranged by size:
709 519 212
NarI GG'CG_CC
Cuts at: 0 1031 1440
Size: 1031 409
NcoI C'CATG_G
Cuts at: 0 365 553 1440
Size: 365 188 887
Fragments arranged by size:
887 365 188
PpuMI rG'GwC_Cy
Cuts at: 0 964 1440
Size: 964 476
PsiI TTA'TAA
Cuts at: 0 124 1440
Size: 124 1316
PstI C_TGCA'G
Cuts at: 0 852 1440
Size: 852 588
PvuII CAG'CTG
Cuts at: 0 709 1228 1440
Size: 709 519 212
Fragments arranged by size:
709 519 212
SanDI GG'GwC_CC
Cuts at: 0 964 1440
Size: 964 476
ScaI AGT'ACT
Cuts at: 0 1393 1440
Size: 1393 47
SfcI C'TryA_G
Cuts at: 0 488 848 1440
Size: 488 360 592
Fragments arranged by size:
592 488 360
SfoI GGC'GCC
Cuts at: 0 1032 1440
Size: 1032 408
SspI AAT'ATT
Cuts at: 0 765 1440
Size: 765 675
StyI C'CwwG_G
Cuts at: 0 365 553 1127 1440
Size: 365 188 574 313
Fragments arranged by size:
574 365 313 188
TatI w'GTAC_w
Cuts at: 0 107 986 1391 1440
Size: 107 879 405 49
Fragments arranged by size:
879 405 107 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 179 211 1440
Size: 179 32 1229
Fragments arranged by size:
1229 179 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 706 1330 1440
Size: 706 624 110
Fragments arranged by size:
706 624 110
ZraI GAC'GTC
Cuts at: 0 310 1440
Size: 310 1130
Enzymes that do cut:
AatII AcuI AleI ApaLI ApoI BanI BanII BbeI
BbsI BbvCI BglI BglII Bme1580I BmrI BplI BpmI
Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BsrBI BsrDI BstAPI BstYI BtgI BtsI DraIII EarI
Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI MfeI
MmeI MslI MspA1I NarI NcoI PpuMI PsiI PstI
PvuII SanDI ScaI SfcI SfoI SspI StyI TatI
TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BlpI BmgBI BmtI BpuEI BsaI BsaAI BsaBI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EaeI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII
HpaI KpnI MluI MscI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI SwaI TaqII Tth111I XbaI XhoI XmaI
XmnI