(Linear) (Six-base) MAPSORT of: KIEE6413.seq Check: 2439 from: 1 to: 1332 KIEE6413.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AccI GT'mk_AC Cuts at: 0 758 1332 Size: 758 574 Acc65I G'GTAC_C Cuts at: 0 54 1332 Size: 54 1278 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 418 1332 Size: 418 914 AleI CACnn'nnGTG Cuts at: 0 45 1332 Size: 45 1287 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 139 239 1332 Size: 139 100 1093 Fragments arranged by size: 1093 139 100 AlwNI CAG_nnn'CTG Cuts at: 0 396 1117 1332 Size: 396 721 215 Fragments arranged by size: 721 396 215 ApoI r'AATT_y Cuts at: 0 579 647 843 1332 Size: 579 68 196 489 Fragments arranged by size: 579 489 196 68 AseI AT'TA_AT Cuts at: 0 671 688 1332 Size: 671 17 644 Fragments arranged by size: 671 644 17 AvaI C'yCGr_G Cuts at: 0 412 638 1332 Size: 412 226 694 Fragments arranged by size: 694 412 226 BanI G'GyrC_C Cuts at: 0 9 54 795 1332 Size: 9 45 741 537 Fragments arranged by size: 741 537 45 9 BanII G_rGCy'C Cuts at: 0 383 419 1332 Size: 383 36 913 Fragments arranged by size: 913 383 36 BbsI GAAGACnn'nnnn_ Cuts at: 0 288 738 944 1332 Size: 288 450 206 388 Fragments arranged by size: 450 388 288 206 BciVI GTATCCnnnnn_n' Cuts at: 0 1146 1332 Size: 1146 186 BfrBI ATG'CAT Cuts at: 0 1044 1332 Size: 1044 288 BglI GCCn_nnn'nGGC Cuts at: 0 598 1332 Size: 598 734 BglII A'GATC_T Cuts at: 0 266 1332 Size: 266 1066 Bme1580I G_kGCm'C Cuts at: 0 800 1183 1332 Size: 800 383 149 Fragments arranged by size: 800 383 149 BmrI ACTGGGnnnn_n' Cuts at: 0 65 481 1332 Size: 65 416 851 Fragments arranged by size: 851 416 65 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 469 471 1332 Size: 469 2 861 Fragments arranged by size: 861 469 2 Bpu10I CC'TnA_GC Cuts at: 0 1054 1332 Size: 1054 278 BsaI GGTCTCn'nnnn_ Cuts at: 0 82 442 1332 Size: 82 360 890 Fragments arranged by size: 890 360 82 BsaBI GATnn'nnATC Cuts at: 0 1066 1332 Size: 1066 266 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 184 1332 Size: 184 1148 BseYI C'CCAG_C Cuts at: 0 93 339 1332 Size: 93 246 993 Fragments arranged by size: 993 246 93 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 833 1332 Size: 145 688 499 Fragments arranged by size: 688 499 145 BsmI GAATG_Cn' Cuts at: 0 1295 1332 Size: 1295 37 Bsp1286I G_dGCh'C Cuts at: 0 383 419 800 1183 1332 Size: 383 36 381 383 149 Fragments arranged by size: 383 383 381 149 36 BspHI T'CATG_A Cuts at: 0 611 1265 1332 Size: 611 654 67 Fragments arranged by size: 654 611 67 BsrDI GCAATG_nn' Cuts at: 0 594 1332 Size: 594 738 BsrFI r'CCGG_y Cuts at: 0 1250 1332 Size: 1250 82 BstAPI GCAn_nnn'nTGC Cuts at: 0 1050 1332 Size: 1050 282 BstEII G'GTnAC_C Cuts at: 0 919 1332 Size: 919 413 BstXI CCAn_nnnn'nTGG Cuts at: 0 894 897 957 996 1332 Size: 894 3 60 39 336 Fragments arranged by size: 894 336 60 39 3 BstYI r'GATC_y Cuts at: 0 266 1332 Size: 266 1066 BtsI GCAGTG_nn' Cuts at: 0 1196 1332 Size: 1196 136 EaeI y'GGCC_r Cuts at: 0 206 320 1252 1332 Size: 206 114 932 80 Fragments arranged by size: 932 206 114 80 EarI CTCTTCn'nnn_ Cuts at: 0 137 182 1332 Size: 137 45 1150 Fragments arranged by size: 1150 137 45 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 418 1332 Size: 418 914 EcoNI CCTnn'n_nnAGG Cuts at: 0 40 433 481 1332 Size: 40 393 48 851 Fragments arranged by size: 851 393 48 40 EcoO109I rG'GnC_Cy Cuts at: 0 393 1332 Size: 393 939 HincII GTy'rAC Cuts at: 0 728 759 1332 Size: 728 31 573 Fragments arranged by size: 728 573 31 KpnI G_GTAC'C Cuts at: 0 58 1332 Size: 58 1274 MscI TGG'CCA Cuts at: 0 208 322 1332 Size: 208 114 1010 Fragments arranged by size: 1010 208 114 MslI CAynn'nnrTG Cuts at: 0 45 793 895 1243 1332 Size: 45 748 102 348 89 Fragments arranged by size: 748 348 102 89 45 NsiI A_TGCA'T Cuts at: 0 1046 1332 Size: 1046 286 NspI r_CATG'y Cuts at: 0 315 1332 Size: 315 1017 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 461 493 1332 Size: 461 32 839 Fragments arranged by size: 839 461 32 SalI G'TCGA_C Cuts at: 0 757 1332 Size: 757 575 SmaI CCC'GGG Cuts at: 0 414 1332 Size: 414 918 SrfI GCCC'GGGC Cuts at: 0 414 1332 Size: 414 918 StyI C'CwwG_G Cuts at: 0 134 227 317 876 1332 Size: 134 93 90 559 456 Fragments arranged by size: 559 456 134 93 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 907 1332 Size: 907 425 TatI w'GTAC_w Cuts at: 0 779 1332 Size: 779 553 Tth111I GACn'n_nGTC Cuts at: 0 742 1332 Size: 742 590 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 894 957 1332 Size: 894 63 375 Fragments arranged by size: 894 375 63 XmaI C'CCGG_G Cuts at: 0 412 1332 Size: 412 920 Enzymes that do cut: AccI Acc65I AcuI AleI AlfI AlwNI ApoI AseI AvaI BanI BanII BbsI BciVI BfrBI BglI BglII Bme1580I BmrI BplI Bpu10I BsaI BsaBI BseRI BseYI BsgI BsmI Bsp1286I BspHI BsrDI BsrFI BstAPI BstEII BstXI BstYI BtsI EaeI EarI Eco57MI EcoNI EcoO109I HincII KpnI MscI MslI NsiI NspI PpiI SalI SmaI SrfI StyI TaqII TatI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AclI AfeI AflII AflIII AgeI AhdI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BclI BlpI BmgBI BmtI BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI BsrBI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI TstI XbaI XhoI XmnI ZraI