Restriction map for
pFN21AE6413
(Linear) (Six-base) MAPSORT of: KIEE6413.seq Check: 2439 from: 1 to: 1332
KIEE6413.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AccI GT'mk_AC
Cuts at: 0 758 1332
Size: 758 574
Acc65I G'GTAC_C
Cuts at: 0 54 1332
Size: 54 1278
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1332
Size: 418 914
AleI CACnn'nnGTG
Cuts at: 0 45 1332
Size: 45 1287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 139 239 1332
Size: 139 100 1093
Fragments arranged by size:
1093 139 100
AlwNI CAG_nnn'CTG
Cuts at: 0 396 1117 1332
Size: 396 721 215
Fragments arranged by size:
721 396 215
ApoI r'AATT_y
Cuts at: 0 579 647 843 1332
Size: 579 68 196 489
Fragments arranged by size:
579 489 196 68
AseI AT'TA_AT
Cuts at: 0 671 688 1332
Size: 671 17 644
Fragments arranged by size:
671 644 17
AvaI C'yCGr_G
Cuts at: 0 412 638 1332
Size: 412 226 694
Fragments arranged by size:
694 412 226
BanI G'GyrC_C
Cuts at: 0 9 54 795 1332
Size: 9 45 741 537
Fragments arranged by size:
741 537 45 9
BanII G_rGCy'C
Cuts at: 0 383 419 1332
Size: 383 36 913
Fragments arranged by size:
913 383 36
BbsI GAAGACnn'nnnn_
Cuts at: 0 288 738 944 1332
Size: 288 450 206 388
Fragments arranged by size:
450 388 288 206
BciVI GTATCCnnnnn_n'
Cuts at: 0 1146 1332
Size: 1146 186
BfrBI ATG'CAT
Cuts at: 0 1044 1332
Size: 1044 288
BglI GCCn_nnn'nGGC
Cuts at: 0 598 1332
Size: 598 734
BglII A'GATC_T
Cuts at: 0 266 1332
Size: 266 1066
Bme1580I G_kGCm'C
Cuts at: 0 800 1183 1332
Size: 800 383 149
Fragments arranged by size:
800 383 149
BmrI ACTGGGnnnn_n'
Cuts at: 0 65 481 1332
Size: 65 416 851
Fragments arranged by size:
851 416 65
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 469 471 1332
Size: 469 2 861
Fragments arranged by size:
861 469 2
Bpu10I CC'TnA_GC
Cuts at: 0 1054 1332
Size: 1054 278
BsaI GGTCTCn'nnnn_
Cuts at: 0 82 442 1332
Size: 82 360 890
Fragments arranged by size:
890 360 82
BsaBI GATnn'nnATC
Cuts at: 0 1066 1332
Size: 1066 266
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 1332
Size: 184 1148
BseYI C'CCAG_C
Cuts at: 0 93 339 1332
Size: 93 246 993
Fragments arranged by size:
993 246 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 833 1332
Size: 145 688 499
Fragments arranged by size:
688 499 145
BsmI GAATG_Cn'
Cuts at: 0 1295 1332
Size: 1295 37
Bsp1286I G_dGCh'C
Cuts at: 0 383 419 800 1183 1332
Size: 383 36 381 383 149
Fragments arranged by size:
383 383 381 149 36
BspHI T'CATG_A
Cuts at: 0 611 1265 1332
Size: 611 654 67
Fragments arranged by size:
654 611 67
BsrDI GCAATG_nn'
Cuts at: 0 594 1332
Size: 594 738
BsrFI r'CCGG_y
Cuts at: 0 1250 1332
Size: 1250 82
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1050 1332
Size: 1050 282
BstEII G'GTnAC_C
Cuts at: 0 919 1332
Size: 919 413
BstXI CCAn_nnnn'nTGG
Cuts at: 0 894 897 957 996 1332
Size: 894 3 60 39 336
Fragments arranged by size:
894 336 60 39 3
BstYI r'GATC_y
Cuts at: 0 266 1332
Size: 266 1066
BtsI GCAGTG_nn'
Cuts at: 0 1196 1332
Size: 1196 136
EaeI y'GGCC_r
Cuts at: 0 206 320 1252 1332
Size: 206 114 932 80
Fragments arranged by size:
932 206 114 80
EarI CTCTTCn'nnn_
Cuts at: 0 137 182 1332
Size: 137 45 1150
Fragments arranged by size:
1150 137 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1332
Size: 418 914
EcoNI CCTnn'n_nnAGG
Cuts at: 0 40 433 481 1332
Size: 40 393 48 851
Fragments arranged by size:
851 393 48 40
EcoO109I rG'GnC_Cy
Cuts at: 0 393 1332
Size: 393 939
HincII GTy'rAC
Cuts at: 0 728 759 1332
Size: 728 31 573
Fragments arranged by size:
728 573 31
KpnI G_GTAC'C
Cuts at: 0 58 1332
Size: 58 1274
MscI TGG'CCA
Cuts at: 0 208 322 1332
Size: 208 114 1010
Fragments arranged by size:
1010 208 114
MslI CAynn'nnrTG
Cuts at: 0 45 793 895 1243 1332
Size: 45 748 102 348 89
Fragments arranged by size:
748 348 102 89 45
NsiI A_TGCA'T
Cuts at: 0 1046 1332
Size: 1046 286
NspI r_CATG'y
Cuts at: 0 315 1332
Size: 315 1017
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 461 493 1332
Size: 461 32 839
Fragments arranged by size:
839 461 32
SalI G'TCGA_C
Cuts at: 0 757 1332
Size: 757 575
SmaI CCC'GGG
Cuts at: 0 414 1332
Size: 414 918
SrfI GCCC'GGGC
Cuts at: 0 414 1332
Size: 414 918
StyI C'CwwG_G
Cuts at: 0 134 227 317 876 1332
Size: 134 93 90 559 456
Fragments arranged by size:
559 456 134 93 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 907 1332
Size: 907 425
TatI w'GTAC_w
Cuts at: 0 779 1332
Size: 779 553
Tth111I GACn'n_nGTC
Cuts at: 0 742 1332
Size: 742 590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 894 957 1332
Size: 894 63 375
Fragments arranged by size:
894 375 63
XmaI C'CCGG_G
Cuts at: 0 412 1332
Size: 412 920
Enzymes that do cut:
AccI Acc65I AcuI AleI AlfI AlwNI ApoI AseI
AvaI BanI BanII BbsI BciVI BfrBI BglI BglII
Bme1580I BmrI BplI Bpu10I BsaI BsaBI BseRI BseYI
BsgI BsmI Bsp1286I BspHI BsrDI BsrFI BstAPI BstEII
BstXI BstYI BtsI EaeI EarI Eco57MI EcoNI EcoO109I
HincII KpnI MscI MslI NsiI NspI PpiI SalI
SmaI SrfI StyI TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BclI BlpI BmgBI BmtI BpmI
BpuEI BsaAI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspMI BsrBI BsrGI BssHII BssSI BstBI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI MfeI
MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SspI StuI SwaI TstI
XbaI XhoI XmnI ZraI