Restriction map for
pFN21AE6412
(Linear) (Six-base) MAPSORT of: KIEE6412.seq Check: 1984 from: 1 to: 1806
KIEE6412.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AccI GT'mk_AC
Cuts at: 0 380 1806
Size: 380 1426
Acc65I G'GTAC_C
Cuts at: 0 520 1806
Size: 520 1286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 1662 1806
Size: 405 1257 144
Fragments arranged by size:
1257 405 144
AfeI AGC'GCT
Cuts at: 0 1202 1423 1806
Size: 1202 221 383
Fragments arranged by size:
1202 383 221
AflIII A'CryG_T
Cuts at: 0 811 1211 1223 1322 1571 1806
Size: 811 400 12 99 249 235
Fragments arranged by size:
811 400 249 235 99 12
AlwNI CAG_nnn'CTG
Cuts at: 0 136 600 938 1063 1806
Size: 136 464 338 125 743
Fragments arranged by size:
743 464 338 136 125
ApaI G_GGCC'C
Cuts at: 0 408 609 1675 1806
Size: 408 201 1066 131
Fragments arranged by size:
1066 408 201 131
ApaLI G'TGCA_C
Cuts at: 0 1509 1806
Size: 1509 297
ApoI r'AATT_y
Cuts at: 0 782 1806
Size: 782 1024
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 34 67 1806
Size: 34 33 1739
Fragments arranged by size:
1739 34 33
BamHI G'GATC_C
Cuts at: 0 590 822 1806
Size: 590 232 984
Fragments arranged by size:
984 590 232
BanI G'GyrC_C
Cuts at: 0 82 202 520 847 884 967 1032 1806
Size: 82 120 318 327 37 83 65 774
Fragments arranged by size:
774 327 318 120 83 82 65 37
BanII G_rGCy'C
Cuts at: 0 408 609 1675 1806
Size: 408 201 1066 131
Fragments arranged by size:
1066 408 201 131
BbsI GAAGACnn'nnnn_
Cuts at: 0 219 246 505 1448 1806
Size: 219 27 259 943 358
Fragments arranged by size:
943 358 259 219 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1727 1761 1806
Size: 1727 34 45
Fragments arranged by size:
1727 45 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 62 1806
Size: 62 1744
BclI T'GATC_A
Cuts at: 0 1295 1806
Size: 1295 511
BglI GCCn_nnn'nGGC
Cuts at: 0 296 1806
Size: 296 1510
BglII A'GATC_T
Cuts at: 0 317 578 1806
Size: 317 261 1228
Fragments arranged by size:
1228 317 261
Bme1580I G_kGCm'C
Cuts at: 0 101 408 609 1037 1513 1675 1708 1806
Size: 101 307 201 428 476 162 33 98
Fragments arranged by size:
476 428 307 201 162 101 98 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 186 283 330 1662 1806
Size: 109 77 97 47 1332 144
Fragments arranged by size:
1332 144 109 97 77 47
Bpu10I CC'TnA_GC
Cuts at: 0 153 1806
Size: 153 1653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1651 1806
Size: 1651 155
BsaI GGTCTCn'nnnn_
Cuts at: 0 18 1806
Size: 18 1788
BsaAI yAC'GTr
Cuts at: 0 1224 1323 1574 1806
Size: 1224 99 251 232
Fragments arranged by size:
1224 251 232 99
BsaBI GATnn'nnATC
Cuts at: 0 364 1806
Size: 364 1442
BsaHI Gr'CG_yC
Cuts at: 0 299 1806
Size: 299 1507
BsaWI w'CCGG_w
Cuts at: 0 1241 1806
Size: 1241 565
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1256 1286 1806
Size: 1256 30 520
Fragments arranged by size:
1256 520 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 361 1106 1806
Size: 361 745 700
Fragments arranged by size:
745 700 361
BseYI C'CCAG_C
Cuts at: 0 136 614 1707 1724 1734 1806
Size: 136 478 1093 17 10 72
Fragments arranged by size:
1093 478 136 72 17 10
BsiHKAI G_wGCw'C
Cuts at: 0 1513 1806
Size: 1513 293
BsmI GAATG_Cn'
Cuts at: 0 245 537 1631 1806
Size: 245 292 1094 175
Fragments arranged by size:
1094 292 245 175
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1281 1806
Size: 1281 525
Bsp1286I G_dGCh'C
Cuts at: 0 101 408 609 1037 1513 1675 1708 1806
Size: 101 307 201 428 476 162 33 98
Fragments arranged by size:
476 428 307 201 162 101 98 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 449 1806
Size: 449 1357
BsrBI CCG'CTC
Cuts at: 0 93 564 1806
Size: 93 471 1242
Fragments arranged by size:
1242 471 93
BsrFI r'CCGG_y
Cuts at: 0 982 1806
Size: 982 824
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1032 1806
Size: 1032 774
BstXI CCAn_nnnn'nTGG
Cuts at: 0 978 1806
Size: 978 828
BstYI r'GATC_y
Cuts at: 0 317 578 590 822 1806
Size: 317 261 12 232 984
Fragments arranged by size:
984 317 261 232 12
BtgI C'CryG_G
Cuts at: 0 1087 1462 1579 1622 1806
Size: 1087 375 117 43 184
Fragments arranged by size:
1087 375 184 117 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 927 1062 1604 1806
Size: 927 135 542 202
Fragments arranged by size:
927 542 202 135
BtsI GCAGTG_nn'
Cuts at: 0 1771 1800 1806
Size: 1771 29 6
Fragments arranged by size:
1771 29 6
EaeI y'GGCC_r
Cuts at: 0 710 980 1049 1349 1376 1806
Size: 710 270 69 300 27 430
Fragments arranged by size:
710 430 300 270 69 27
EarI CTCTTCn'nnn_
Cuts at: 0 424 1495 1531 1806
Size: 424 1071 36 275
Fragments arranged by size:
1071 424 275 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1328 1806
Size: 1328 478
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 186 283 330 405 1662 1806
Size: 109 77 97 47 75 1257 144
Fragments arranged by size:
1257 144 109 97 77 75 47
EcoO109I rG'GnC_Cy
Cuts at: 0 405 605 606 1060 1671 1672 1741 1766
Size: 405 200 1 454 611 1 69 25
Cuts at: 1766 1806
Size: 40
Fragments arranged by size:
611 454 405 200 69 40 25 1
1
EcoRI G'AATT_C
Cuts at: 0 782 1806
Size: 782 1024
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1692 1806
Size: 1692 114
HaeII r_GCGC'y
Cuts at: 0 1204 1425 1806
Size: 1204 221 381
Fragments arranged by size:
1204 381 221
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1256 1288 1673 1677 1705 1709 1780 1806
Size: 1256 32 385 4 28 4 71 26
Fragments arranged by size:
1256 385 71 32 28 26 4 4
KpnI G_GTAC'C
Cuts at: 0 524 1806
Size: 524 1282
MluI A'CGCG_T
Cuts at: 0 811 1806
Size: 811 995
MscI TGG'CCA
Cuts at: 0 1378 1806
Size: 1378 428
MslI CAynn'nnrTG
Cuts at: 0 391 733 1806
Size: 391 342 1073
Fragments arranged by size:
1073 391 342
MspA1I CmG'CkG
Cuts at: 0 965 1089 1504 1551 1728 1806
Size: 965 124 415 47 177 78
Fragments arranged by size:
965 415 177 124 78 47
NaeI GCC'GGC
Cuts at: 0 984 1806
Size: 984 822
NcoI C'CATG_G
Cuts at: 0 1462 1622 1806
Size: 1462 160 184
Fragments arranged by size:
1462 184 160
NgoMIV G'CCGG_C
Cuts at: 0 982 1806
Size: 982 824
NspI r_CATG'y
Cuts at: 0 1215 1362 1806
Size: 1215 147 444
Fragments arranged by size:
1215 444 147
PasI CC'CwG_GG
Cuts at: 0 446 897 1596 1806
Size: 446 451 699 210
Fragments arranged by size:
699 451 446 210
PciI A'CATG_T
Cuts at: 0 1211 1806
Size: 1211 595
PflMI CCAn_nnn'nTGG
Cuts at: 0 600 1806
Size: 600 1206
PmlI CAC'GTG
Cuts at: 0 1224 1806
Size: 1224 582
PpuMI rG'GwC_Cy
Cuts at: 0 1741 1766 1806
Size: 1741 25 40
Fragments arranged by size:
1741 40 25
PspOMI G'GGCC_C
Cuts at: 0 404 605 1671 1806
Size: 404 201 1066 135
Fragments arranged by size:
1066 404 201 135
PvuII CAG'CTG
Cuts at: 0 1504 1806
Size: 1504 302
SacII CC_GC'GG
Cuts at: 0 1090 1806
Size: 1090 716
SapI GCTCTTCn'nnn_
Cuts at: 0 1495 1806
Size: 1495 311
SfcI C'TryA_G
Cuts at: 0 930 1650 1806
Size: 930 720 156
Fragments arranged by size:
930 720 156
SmlI C'TyrA_G
Cuts at: 0 1666 1806
Size: 1666 140
StyI C'CwwG_G
Cuts at: 0 777 1260 1462 1622 1806
Size: 777 483 202 160 184
Fragments arranged by size:
777 483 202 184 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 865 872 1806
Size: 865 7 934
Fragments arranged by size:
934 865 7
TatI w'GTAC_w
Cuts at: 0 281 701 1541 1568 1806
Size: 281 420 840 27 238
Fragments arranged by size:
840 420 281 238 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1307 1339 1806
Size: 1307 32 467
Fragments arranged by size:
1307 467 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 693 1044 1806
Size: 693 351 762
Fragments arranged by size:
762 693 351
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AlwNI ApaI ApaLI
ApoI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII Bme1580I BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI
BstYI BtgI BtgZI BtsI EaeI EarI EciI Eco57MI
EcoO109I EcoRI FalI HaeII Hin4I KpnI MluI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI
PflMI PmlI PpuMI PspOMI PvuII SacII SapI SfcI
SmlI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AleI AlfI
AloI AscI AseI AsiSI AvaI AvrII BbeI BbvCI
BfrBI BlpI BmgBI BmrI BmtI BplI BsgI BsiEI
BsiWI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI MfeI MmeI NarI NdeI NheI
NotI NruI NsiI PacI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI