Restriction map for
pFN21AE6411
(Linear) (Six-base) MAPSORT of: KIEE6411.seq Check: 250 from: 1 to: 1806
KIEE6411.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 900 1806
Size: 900 906
AleI CACnn'nnGTG
Cuts at: 0 1144 1806
Size: 1144 662
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1069 1101 1806
Size: 1069 32 705
Fragments arranged by size:
1069 705 32
AlwNI CAG_nnn'CTG
Cuts at: 0 544 923 1002 1806
Size: 544 379 79 804
Fragments arranged by size:
804 544 379 79
ApaI G_GGCC'C
Cuts at: 0 1010 1806
Size: 1010 796
ApaLI G'TGCA_C
Cuts at: 0 156 1510 1806
Size: 156 1354 296
Fragments arranged by size:
1354 296 156
ApoI r'AATT_y
Cuts at: 0 819 1806
Size: 819 987
AvaI C'yCGr_G
Cuts at: 0 971 1073 1806
Size: 971 102 733
Fragments arranged by size:
971 733 102
BanI G'GyrC_C
Cuts at: 0 1720 1806
Size: 1720 86
BanII G_rGCy'C
Cuts at: 0 972 1010 1241 1806
Size: 972 38 231 565
Fragments arranged by size:
972 565 231 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 84 1806
Size: 84 1722
BclI T'GATC_A
Cuts at: 0 290 1806
Size: 290 1516
BglI GCCn_nnn'nGGC
Cuts at: 0 375 1027 1806
Size: 375 652 779
Fragments arranged by size:
779 652 375
BlpI GC'TnA_GC
Cuts at: 0 424 1806
Size: 424 1382
Bme1580I G_kGCm'C
Cuts at: 0 160 575 739 757 1010 1277 1388 1514
Size: 160 415 164 18 253 267 111 126
Cuts at: 1514 1540 1701 1723 1806
Size: 26 161 22 83
Fragments arranged by size:
415 267 253 164 161 160 126 111
83 26 22 18
BmgBI CAC'GTC
Cuts at: 0 813 1806
Size: 813 993
BmrI ACTGGGnnnn_n'
Cuts at: 0 243 1743 1806
Size: 243 1500 63
Fragments arranged by size:
1500 243 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 945 1396 1806
Size: 642 303 451 410
Fragments arranged by size:
642 451 410 303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 772 1180 1806
Size: 325 447 408 626
Fragments arranged by size:
626 447 408 325
BsaI GGTCTCn'nnnn_
Cuts at: 0 1578 1806
Size: 1578 228
BsaBI GATnn'nnATC
Cuts at: 0 674 1806
Size: 674 1132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 421 451 571 601 1806
Size: 421 30 120 30 1205
Fragments arranged by size:
1205 421 120 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 325 1054 1806
Size: 325 729 752
Fragments arranged by size:
752 729 325
BseYI C'CCAG_C
Cuts at: 0 514 1097 1716 1806
Size: 514 583 619 90
Fragments arranged by size:
619 583 514 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1351 1806
Size: 307 1044 455
Fragments arranged by size:
1044 455 307
BsiHKAI G_wGCw'C
Cuts at: 0 160 1514 1806
Size: 160 1354 292
Fragments arranged by size:
1354 292 160
Bsp1286I G_dGCh'C
Cuts at: 0 160 575 739 757 972 1010 1241 1277
Size: 160 415 164 18 215 38 231 36
Cuts at: 1277 1388 1514 1540 1701 1723 1806
Size: 111 126 26 161 22 83
Fragments arranged by size:
415 231 215 164 161 160 126 111
83 38 36 26 22 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 329 1806
Size: 329 1477
BsrBI CCG'CTC
Cuts at: 0 72 174 1806
Size: 72 102 1632
Fragments arranged by size:
1632 102 72
BsrDI GCAATG_nn'
Cuts at: 0 458 1765 1806
Size: 458 1307 41
Fragments arranged by size:
1307 458 41
BsrFI r'CCGG_y
Cuts at: 0 84 326 369 648 1141 1476 1806
Size: 84 242 43 279 493 335 330
Fragments arranged by size:
493 335 330 279 242 84 43
BssHII G'CGCG_C
Cuts at: 0 38 1806
Size: 38 1768
BstBI TT'CG_AA
Cuts at: 0 504 1806
Size: 504 1302
BstEII G'GTnAC_C
Cuts at: 0 396 1806
Size: 396 1410
BstYI r'GATC_y
Cuts at: 0 344 642 887 1806
Size: 344 298 245 919
Fragments arranged by size:
919 344 298 245
BtgI C'CryG_G
Cuts at: 0 575 1313 1806
Size: 575 738 493
Fragments arranged by size:
738 575 493
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1407 1578 1806
Size: 1407 171 228
Fragments arranged by size:
1407 228 171
BtsI GCAGTG_nn'
Cuts at: 0 440 811 1806
Size: 440 371 995
Fragments arranged by size:
995 440 371
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1760 1795 1806
Size: 1760 35 11
Fragments arranged by size:
1760 35 11
DraIII CAC_nnn'GTG
Cuts at: 0 570 1806
Size: 570 1236
EaeI y'GGCC_r
Cuts at: 0 20 296 371 489 780 1316 1493 1781
Size: 20 276 75 118 291 536 177 288
Cuts at: 1781 1806
Size: 25
Fragments arranged by size:
536 291 288 276 177 118 75 25
20
EarI CTCTTCn'nnn_
Cuts at: 0 280 524 1072 1114 1806
Size: 280 244 548 42 692
Fragments arranged by size:
692 548 280 244 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1489 1806
Size: 1489 317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 900 945 1396 1806
Size: 642 258 45 451 410
Fragments arranged by size:
642 451 410 258 45
EcoO109I rG'GnC_Cy
Cuts at: 0 165 510 1006 1077 1187 1806
Size: 165 345 496 71 110 619
Fragments arranged by size:
619 496 345 165 110 71
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 813 1806
Size: 813 993
HaeII r_GCGC'y
Cuts at: 0 10 190 305 1806
Size: 10 180 115 1501
Fragments arranged by size:
1501 180 115 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1732 1764 1806
Size: 1732 32 42
Fragments arranged by size:
1732 42 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 339 661 1806
Size: 257 82 322 1145
Fragments arranged by size:
1145 322 257 82
MscI TGG'CCA
Cuts at: 0 298 491 782 1318 1495 1783 1806
Size: 298 193 291 536 177 288 23
Fragments arranged by size:
536 298 291 288 193 177 23
MslI CAynn'nnrTG
Cuts at: 0 145 445 580 1144 1414 1806
Size: 145 300 135 564 270 392
Fragments arranged by size:
564 392 300 270 145 135
MspA1I CmG'CkG
Cuts at: 0 19 119 541 1365 1716 1806
Size: 19 100 422 824 351 90
Fragments arranged by size:
824 422 351 100 90 19
NaeI GCC'GGC
Cuts at: 0 371 650 1806
Size: 371 279 1156
Fragments arranged by size:
1156 371 279
NcoI C'CATG_G
Cuts at: 0 1313 1806
Size: 1313 493
NgoMIV G'CCGG_C
Cuts at: 0 369 648 1806
Size: 369 279 1158
Fragments arranged by size:
1158 369 279
NspI r_CATG'y
Cuts at: 0 1053 1806
Size: 1053 753
PasI CC'CwG_GG
Cuts at: 0 619 1806
Size: 619 1187
PflMI CCAn_nnn'nTGG
Cuts at: 0 1002 1806
Size: 1002 804
PfoI T'CCnGG_A
Cuts at: 0 658 1727 1806
Size: 658 1069 79
Fragments arranged by size:
1069 658 79
PpuMI rG'GwC_Cy
Cuts at: 0 510 1077 1806
Size: 510 567 729
Fragments arranged by size:
729 567 510
PspOMI G'GGCC_C
Cuts at: 0 1006 1806
Size: 1006 800
PspXI vC'TCGA_Gb
Cuts at: 0 971 1806
Size: 971 835
PvuII CAG'CTG
Cuts at: 0 541 1365 1716 1806
Size: 541 824 351 90
Fragments arranged by size:
824 541 351 90
SanDI GG'GwC_CC
Cuts at: 0 1077 1806
Size: 1077 729
ScaI AGT'ACT
Cuts at: 0 253 1806
Size: 253 1553
SgrAI Cr'CCGG_yG
Cuts at: 0 326 1141 1806
Size: 326 815 665
Fragments arranged by size:
815 665 326
SmaI CCC'GGG
Cuts at: 0 1075 1806
Size: 1075 731
SmlI C'TyrA_G
Cuts at: 0 340 787 971 1195 1806
Size: 340 447 184 224 611
Fragments arranged by size:
611 447 340 224 184
StuI AGG'CCT
Cuts at: 0 1643 1806
Size: 1643 163
StyI C'CwwG_G
Cuts at: 0 107 1313 1778 1806
Size: 107 1206 465 28
Fragments arranged by size:
1206 465 107 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1651 1740 1788 1806
Size: 1651 89 48 18
Fragments arranged by size:
1651 89 48 18
TatI w'GTAC_w
Cuts at: 0 149 251 1745 1806
Size: 149 102 1494 61
Fragments arranged by size:
1494 149 102 61
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 381 1713 1806
Size: 381 1332 93
Fragments arranged by size:
1332 381 93
XhoI C'TCGA_G
Cuts at: 0 971 1806
Size: 971 835
XmaI C'CCGG_G
Cuts at: 0 1073 1806
Size: 1073 733
XmnI GAAnn'nnTTC
Cuts at: 0 697 1806
Size: 697 1109
Enzymes that do cut:
AcuI AleI AloI AlwNI ApaI ApaLI ApoI AvaI
BanI BanII BbsI BclI BglI BlpI Bme1580I BmgBI
BmrI BpmI BpuEI BsaI BsaBI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BstBI BstEII BstYI BtgI BtgZI BtsI CspCI DraIII
EaeI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PflMI PfoI PpuMI PspOMI PspXI PvuII SanDI
ScaI SgrAI SmaI SmlI StuI StyI TaqII TatI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BfrBI BglII BmtI
BplI Bpu10I BsaAI BsaHI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrGI BssSI BstAPI BstXI BstZ17I
Bsu36I ClaI DraI DrdI EagI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PsrI PstI PvuI RsrII SacI SacII SalI SapI
SbfI SexAI SfcI SfiI SfoI SgfI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI ZraI