Restriction map for
pFN21AE6410
(Linear) (Six-base) MAPSORT of: KIEE6410.seq Check: 7032 from: 1 to: 1404
KIEE6410.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 65 345 1404
Size: 65 280 1059
Fragments arranged by size:
1059 280 65
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 1404
Size: 982 422
AhdI GACnn_n'nnGTC
Cuts at: 0 1132 1404
Size: 1132 272
ApoI r'AATT_y
Cuts at: 0 862 1404
Size: 862 542
AvaI C'yCGr_G
Cuts at: 0 1076 1404
Size: 1076 328
AvrII C'CTAG_G
Cuts at: 0 576 1404
Size: 576 828
BanI G'GyrC_C
Cuts at: 0 85 304 931 1404
Size: 85 219 627 473
Fragments arranged by size:
627 473 219 85
BbeI G_GCGC'C
Cuts at: 0 89 1404
Size: 89 1315
BfrBI ATG'CAT
Cuts at: 0 447 1404
Size: 447 957
BglI GCCn_nnn'nGGC
Cuts at: 0 137 1404
Size: 137 1267
BmgBI CAC'GTC
Cuts at: 0 111 818 1337 1404
Size: 111 707 519 67
Fragments arranged by size:
707 519 111 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 537 1143 1404
Size: 247 290 606 261
Fragments arranged by size:
606 290 261 247
Bpu10I CC'TnA_GC
Cuts at: 0 1330 1404
Size: 1330 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 816 1404
Size: 816 588
BsaI GGTCTCn'nnnn_
Cuts at: 0 981 1404
Size: 981 423
BsaHI Gr'CG_yC
Cuts at: 0 86 365 1404
Size: 86 279 1039
Fragments arranged by size:
1039 279 86
BsaWI w'CCGG_w
Cuts at: 0 634 1404
Size: 634 770
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 119 149 220 250 460 490 1404
Size: 119 30 71 30 210 30 914
Fragments arranged by size:
914 210 119 71 30 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 638 1404
Size: 59 579 766
Fragments arranged by size:
766 579 59
BsmI GAATG_Cn'
Cuts at: 0 356 1404
Size: 356 1048
BspEI T'CCGG_A
Cuts at: 0 634 1404
Size: 634 770
BspHI T'CATG_A
Cuts at: 0 180 1404
Size: 180 1224
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 65 199 345 1404
Size: 65 134 146 1059
Fragments arranged by size:
1059 146 134 65
BsrBI CCG'CTC
Cuts at: 0 783 1404
Size: 783 621
BtgI C'CryG_G
Cuts at: 0 146 1404
Size: 146 1258
BtsI GCAGTG_nn'
Cuts at: 0 1259 1367 1404
Size: 1259 108 37
Fragments arranged by size:
1259 108 37
EaeI y'GGCC_r
Cuts at: 0 545 1404
Size: 545 859
EarI CTCTTCn'nnn_
Cuts at: 0 1271 1404
Size: 1271 133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 537 982 1143 1404
Size: 247 290 445 161 261
Fragments arranged by size:
445 290 261 247 161
EcoNI CCTnn'n_nnAGG
Cuts at: 0 72 1404
Size: 72 1332
EcoO109I rG'GnC_Cy
Cuts at: 0 378 1404
Size: 378 1026
EcoRV GAT'ATC
Cuts at: 0 1155 1404
Size: 1155 249
HaeII r_GCGC'y
Cuts at: 0 89 143 1087 1404
Size: 89 54 944 317
Fragments arranged by size:
944 317 89 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 355 387 460 492 1118 1150
Size: 220 32 103 32 73 32 626 32
Cuts at: 1150 1404
Size: 254
Fragments arranged by size:
626 254 220 103 73 32 32 32
32
HincII GTy'rAC
Cuts at: 0 879 1137 1404
Size: 879 258 267
Fragments arranged by size:
879 267 258
HpaI GTT'AAC
Cuts at: 0 879 1404
Size: 879 525
KasI G'GCGC_C
Cuts at: 0 85 1404
Size: 85 1319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 633 1404
Size: 446 187 771
Fragments arranged by size:
771 446 187
MslI CAynn'nnrTG
Cuts at: 0 179 827 1404
Size: 179 648 577
Fragments arranged by size:
648 577 179
MspA1I CmG'CkG
Cuts at: 0 53 60 928 990 1404
Size: 53 7 868 62 414
Fragments arranged by size:
868 414 62 53 7
NarI GG'CG_CC
Cuts at: 0 86 1404
Size: 86 1318
NcoI C'CATG_G
Cuts at: 0 146 1404
Size: 146 1258
NsiI A_TGCA'T
Cuts at: 0 449 1404
Size: 449 955
NspI r_CATG'y
Cuts at: 0 451 832 1057 1404
Size: 451 381 225 347
Fragments arranged by size:
451 381 347 225
PflMI CCAn_nnn'nTGG
Cuts at: 0 560 1404
Size: 560 844
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 476 508 1404
Size: 476 32 896
Fragments arranged by size:
896 476 32
PstI C_TGCA'G
Cuts at: 0 944 1404
Size: 944 460
PvuII CAG'CTG
Cuts at: 0 928 990 1404
Size: 928 62 414
Fragments arranged by size:
928 414 62
SapI GCTCTTCn'nnn_
Cuts at: 0 1271 1404
Size: 1271 133
ScaI AGT'ACT
Cuts at: 0 224 1404
Size: 224 1180
SfcI C'TryA_G
Cuts at: 0 498 940 1277 1404
Size: 498 442 337 127
Fragments arranged by size:
498 442 337 127
SfoI GGC'GCC
Cuts at: 0 87 1404
Size: 87 1317
SmlI C'TyrA_G
Cuts at: 0 795 1404
Size: 795 609
SphI G_CATG'C
Cuts at: 0 451 1404
Size: 451 953
StuI AGG'CCT
Cuts at: 0 131 1074 1404
Size: 131 943 330
Fragments arranged by size:
943 330 131
StyI C'CwwG_G
Cuts at: 0 146 158 559 576 803 1404
Size: 146 12 401 17 227 601
Fragments arranged by size:
601 401 227 146 17 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 497 1404
Size: 497 907
TatI w'GTAC_w
Cuts at: 0 222 1404
Size: 222 1182
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 119 151 1404
Size: 119 32 1253
Fragments arranged by size:
1253 119 32
Tth111I GACn'n_nGTC
Cuts at: 0 1250 1404
Size: 1250 154
Enzymes that do cut:
AarI AcuI AhdI ApoI AvaI AvrII BanI BbeI
BfrBI BglI BmgBI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BsgI BsmI BspEI BspHI BspMI BsrBI
BtgI BtsI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV
HaeII Hin4I HincII HpaI KasI MmeI MslI MspA1I
NarI NcoI NsiI NspI PflMI PpiI PstI PvuII
SapI ScaI SfcI SfoI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI BaeI BamHI BanII BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BmtI BplI BsaAI
BsaBI BseRI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoRI FalI FseI
FspI FspAI HindIII KpnI MfeI MluI MscI NaeI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI XbaI XcmI XhoI XmaI XmnI ZraI