Restriction map for
pFN21AE6408
(Linear) (Six-base) MAPSORT of: KIEE6408.seq Check: 6441 from: 1 to: 1599
KIEE6408.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 561 1599
Size: 561 1038
AccI GT'mk_AC
Cuts at: 0 912 1599
Size: 912 687
Acc65I G'GTAC_C
Cuts at: 0 935 1599
Size: 935 664
AclI AA'CG_TT
Cuts at: 0 781 1599
Size: 781 818
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1599
Size: 400 1199
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 605 1599
Size: 605 994
AlwNI CAG_nnn'CTG
Cuts at: 0 129 220 627 802 1599
Size: 129 91 407 175 797
Fragments arranged by size:
797 407 175 129 91
ApaI G_GGCC'C
Cuts at: 0 957 1599
Size: 957 642
ApoI r'AATT_y
Cuts at: 0 64 1599
Size: 64 1535
AvaI C'yCGr_G
Cuts at: 0 1187 1599
Size: 1187 412
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1427 1460 1599
Size: 1427 33 139
Fragments arranged by size:
1427 139 33
BanI G'GyrC_C
Cuts at: 0 935 1387 1599
Size: 935 452 212
Fragments arranged by size:
935 452 212
BanII G_rGCy'C
Cuts at: 0 686 957 1599
Size: 686 271 642
Fragments arranged by size:
686 642 271
BbsI GAAGACnn'nnnn_
Cuts at: 0 642 1389 1599
Size: 642 747 210
Fragments arranged by size:
747 642 210
BbvCI CC'TCA_GC
Cuts at: 0 227 637 1599
Size: 227 410 962
Fragments arranged by size:
962 410 227
BlpI GC'TnA_GC
Cuts at: 0 803 1599
Size: 803 796
Bme1580I G_kGCm'C
Cuts at: 0 86 678 957 1599
Size: 86 592 279 642
Fragments arranged by size:
642 592 279 86
BmtI G_CTAG'C
Cuts at: 0 811 1599
Size: 811 788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 700 749 1599
Size: 319 381 49 850
Fragments arranged by size:
850 381 319 49
Bpu10I CC'TnA_GC
Cuts at: 0 46 227 637 1599
Size: 46 181 410 962
Fragments arranged by size:
962 410 181 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1590 1599
Size: 1590 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 1536 1566 1599
Size: 1536 30 33
Fragments arranged by size:
1536 33 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 979 1009 1599
Size: 979 30 590
Fragments arranged by size:
979 590 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 613 1000 1599
Size: 613 387 599
Fragments arranged by size:
613 599 387
BseYI C'CCAG_C
Cuts at: 0 179 1599
Size: 179 1420
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 142 373 652 1599
Size: 76 66 231 279 947
Fragments arranged by size:
947 279 231 76 66
BsiHKAI G_wGCw'C
Cuts at: 0 686 1599
Size: 686 913
BsmI GAATG_Cn'
Cuts at: 0 258 1599
Size: 258 1341
Bsp1286I G_dGCh'C
Cuts at: 0 86 678 686 957 1599
Size: 86 592 8 271 642
Fragments arranged by size:
642 592 271 86 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 319 561 857 1599
Size: 319 242 296 742
Fragments arranged by size:
742 319 296 242
BsrBI CCG'CTC
Cuts at: 0 521 1469 1599
Size: 521 948 130
Fragments arranged by size:
948 521 130
BsrDI GCAATG_nn'
Cuts at: 0 19 491 968 1599
Size: 19 472 477 631
Fragments arranged by size:
631 477 472 19
BssSI C'ACGA_G
Cuts at: 0 1085 1599
Size: 1085 514
BstXI CCAn_nnnn'nTGG
Cuts at: 0 192 1599
Size: 192 1407
BtgI C'CryG_G
Cuts at: 0 942 1599
Size: 942 657
DraIII CAC_nnn'GTG
Cuts at: 0 249 1599
Size: 249 1350
DrdI GACnn_nn'nnGTC
Cuts at: 0 210 1599
Size: 210 1389
EarI CTCTTCn'nnn_
Cuts at: 0 761 913 1599
Size: 761 152 686
Fragments arranged by size:
761 686 152
EcoICRI GAG'CTC
Cuts at: 0 684 1599
Size: 684 915
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 400 700 749 1599
Size: 319 81 300 49 850
Fragments arranged by size:
850 319 300 81 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 148 1599
Size: 148 1451
EcoO109I rG'GnC_Cy
Cuts at: 0 303 953 954 1599
Size: 303 650 1 645
Fragments arranged by size:
650 645 303 1
EcoRI G'AATT_C
Cuts at: 0 64 1599
Size: 64 1535
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 178 1599
Size: 178 1421
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 864 896 1018 1050 1599
Size: 864 32 122 32 549
Fragments arranged by size:
864 549 122 32 32
HincII GTy'rAC
Cuts at: 0 785 1223 1596 1599
Size: 785 438 373 3
Fragments arranged by size:
785 438 373 3
HindIII A'AGCT_T
Cuts at: 0 160 1599
Size: 160 1439
HpaI GTT'AAC
Cuts at: 0 785 1223 1599
Size: 785 438 376
Fragments arranged by size:
785 438 376
KpnI G_GTAC'C
Cuts at: 0 939 1599
Size: 939 660
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1180 1599
Size: 1180 419
MslI CAynn'nnrTG
Cuts at: 0 145 1599
Size: 145 1454
MspA1I CmG'CkG
Cuts at: 0 217 325 357 963 1319 1441 1599
Size: 217 108 32 606 356 122 158
Fragments arranged by size:
606 356 217 158 122 108 32
NcoI C'CATG_G
Cuts at: 0 942 1599
Size: 942 657
NheI G'CTAG_C
Cuts at: 0 807 1599
Size: 807 792
NspI r_CATG'y
Cuts at: 0 1498 1599
Size: 1498 101
PflMI CCAn_nnn'nTGG
Cuts at: 0 1541 1599
Size: 1541 58
PfoI T'CCnGG_A
Cuts at: 0 465 1599
Size: 465 1134
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1079 1111 1599
Size: 1079 32 488
Fragments arranged by size:
1079 488 32
PpuMI rG'GwC_Cy
Cuts at: 0 303 1599
Size: 303 1296
PshAI GACnn'nnGTC
Cuts at: 0 1114 1599
Size: 1114 485
PspOMI G'GGCC_C
Cuts at: 0 953 1599
Size: 953 646
PstI C_TGCA'G
Cuts at: 0 317 330 1239 1599
Size: 317 13 909 360
Fragments arranged by size:
909 360 317 13
PvuII CAG'CTG
Cuts at: 0 217 325 963 1441 1599
Size: 217 108 638 478 158
Fragments arranged by size:
638 478 217 158 108
SacI G_AGCT'C
Cuts at: 0 686 1599
Size: 686 913
SbfI CC_TGCA'GG
Cuts at: 0 317 1599
Size: 317 1282
SfcI C'TryA_G
Cuts at: 0 313 326 930 1235 1599
Size: 313 13 604 305 364
Fragments arranged by size:
604 364 313 305 13
SmlI C'TyrA_G
Cuts at: 0 1569 1599
Size: 1569 30
StuI AGG'CCT
Cuts at: 0 837 1599
Size: 837 762
StyI C'CwwG_G
Cuts at: 0 942 1599
Size: 942 657
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 649 1599
Size: 649 950
TatI w'GTAC_w
Cuts at: 0 137 1599
Size: 137 1462
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 979 1011 1599
Size: 979 32 588
Fragments arranged by size:
979 588 32
Tth111I GACn'n_nGTC
Cuts at: 0 307 1599
Size: 307 1292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 724 1599
Size: 724 875
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AlfI AlwNI ApaI
ApoI AvaI BaeI BanI BanII BbsI BbvCI BlpI
Bme1580I BmtI BpmI Bpu10I BpuEI BsaI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BssSI BstXI BtgI DraIII DrdI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII HpaI
KpnI MmeI MslI MspA1I NcoI NheI NspI PflMI
PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacI
SbfI SfcI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI
Enzymes that do not cut:
AatII AfeI AflII AflIII AgeI AhdI AleI AloI
ApaLI AscI AseI AsiSI AvrII BamHI BbeI BcgI
BciVI BclI BfrBI BglI BglII BmgBI BmrI BplI
BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI
BspHI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI EaeI
EagI EciI EcoRV FseI FspI FspAI HaeII KasI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PasI PciI PmeI PmlI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI
XmnI ZraI