Restriction map for
pFN21AE6407
(Linear) (Six-base) MAPSORT of: KIEE6407.seq Check: 3958 from: 1 to: 1488
KIEE6407.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 1488
Size: 704 784
AflIII A'CryG_T
Cuts at: 0 497 1488
Size: 497 991
AleI CACnn'nnGTG
Cuts at: 0 665 1488
Size: 665 823
AlwNI CAG_nnn'CTG
Cuts at: 0 32 1488
Size: 32 1456
ApoI r'AATT_y
Cuts at: 0 1352 1488
Size: 1352 136
AvaI C'yCGr_G
Cuts at: 0 397 1488
Size: 397 1091
BanI G'GyrC_C
Cuts at: 0 918 1488
Size: 918 570
BanII G_rGCy'C
Cuts at: 0 1292 1417 1488
Size: 1292 125 71
Fragments arranged by size:
1292 125 71
BbeI G_GCGC'C
Cuts at: 0 922 1488
Size: 922 566
BbsI GAAGACnn'nnnn_
Cuts at: 0 316 1044 1488
Size: 316 728 444
Fragments arranged by size:
728 444 316
BciVI GTATCCnnnnn_n'
Cuts at: 0 495 1488
Size: 495 993
BfrBI ATG'CAT
Cuts at: 0 1089 1488
Size: 1089 399
BglI GCCn_nnn'nGGC
Cuts at: 0 361 1488
Size: 361 1127
BglII A'GATC_T
Cuts at: 0 161 1488
Size: 161 1327
BmrI ACTGGGnnnn_n'
Cuts at: 0 1029 1488
Size: 1029 459
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 834 1436 1488
Size: 834 602 52
Fragments arranged by size:
834 602 52
BsaI GGTCTCn'nnnn_
Cuts at: 0 78 848 1488
Size: 78 770 640
Fragments arranged by size:
770 640 78
BsaHI Gr'CG_yC
Cuts at: 0 919 1488
Size: 919 569
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 968 998 1488
Size: 968 30 490
Fragments arranged by size:
968 490 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 810 1488
Size: 810 678
BseYI C'CCAG_C
Cuts at: 0 607 1488
Size: 607 881
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 808 1488
Size: 164 644 680
Fragments arranged by size:
680 644 164
BsiHKAI G_wGCw'C
Cuts at: 0 404 1488
Size: 404 1084
BsmI GAATG_Cn'
Cuts at: 0 1307 1488
Size: 1307 181
Bsp1286I G_dGCh'C
Cuts at: 0 404 1292 1417 1488
Size: 404 888 125 71
Fragments arranged by size:
888 404 125 71
BspHI T'CATG_A
Cuts at: 0 1259 1488
Size: 1259 229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 309 859 1488
Size: 309 550 629
Fragments arranged by size:
629 550 309
BsrBI CCG'CTC
Cuts at: 0 419 1488
Size: 419 1069
BsrDI GCAATG_nn'
Cuts at: 0 771 1488
Size: 771 717
BsrFI r'CCGG_y
Cuts at: 0 1069 1488
Size: 1069 419
BsrGI T'GTAC_A
Cuts at: 0 1407 1488
Size: 1407 81
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1345 1488
Size: 1345 143
BstEII G'GTnAC_C
Cuts at: 0 345 1488
Size: 345 1143
BstYI r'GATC_y
Cuts at: 0 161 1488
Size: 161 1327
BtgI C'CryG_G
Cuts at: 0 542 1488
Size: 542 946
BtsI GCAGTG_nn'
Cuts at: 0 796 1004 1488
Size: 796 208 484
Fragments arranged by size:
796 484 208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 886 921 1488
Size: 886 35 567
Fragments arranged by size:
886 567 35
EaeI y'GGCC_r
Cuts at: 0 297 443 1488
Size: 297 146 1045
Fragments arranged by size:
1045 297 146
EarI CTCTTCn'nnn_
Cuts at: 0 680 1451 1488
Size: 680 771 37
Fragments arranged by size:
771 680 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 1488
Size: 704 784
EcoO109I rG'GnC_Cy
Cuts at: 0 1023 1488
Size: 1023 465
HaeII r_GCGC'y
Cuts at: 0 922 1488
Size: 922 566
HincII GTy'rAC
Cuts at: 0 1017 1478 1488
Size: 1017 461 10
Fragments arranged by size:
1017 461 10
KasI G'GCGC_C
Cuts at: 0 918 1488
Size: 918 570
MscI TGG'CCA
Cuts at: 0 299 1488
Size: 299 1189
MslI CAynn'nnrTG
Cuts at: 0 665 1264 1488
Size: 665 599 224
Fragments arranged by size:
665 599 224
MspA1I CmG'CkG
Cuts at: 0 744 1488
Size: 744 744
NarI GG'CG_CC
Cuts at: 0 919 1488
Size: 919 569
NsiI A_TGCA'T
Cuts at: 0 1091 1488
Size: 1091 397
NspI r_CATG'y
Cuts at: 0 501 591 1374 1488
Size: 501 90 783 114
Fragments arranged by size:
783 501 114 90
PciI A'CATG_T
Cuts at: 0 497 1488
Size: 497 991
PflMI CCAn_nnn'nTGG
Cuts at: 0 1106 1488
Size: 1106 382
PfoI T'CCnGG_A
Cuts at: 0 31 1488
Size: 31 1457
PpuMI rG'GwC_Cy
Cuts at: 0 1023 1488
Size: 1023 465
PsiI TTA'TAA
Cuts at: 0 1159 1488
Size: 1159 329
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 487 519 1488
Size: 487 32 969
Fragments arranged by size:
969 487 32
PstI C_TGCA'G
Cuts at: 0 430 1488
Size: 430 1058
PvuII CAG'CTG
Cuts at: 0 744 1488
Size: 744 744
SapI GCTCTTCn'nnn_
Cuts at: 0 680 1488
Size: 680 808
SexAI A'CCwGG_T
Cuts at: 0 188 878 1488
Size: 188 690 610
Fragments arranged by size:
690 610 188
SfcI C'TryA_G
Cuts at: 0 426 1488
Size: 426 1062
SfoI GGC'GCC
Cuts at: 0 920 1488
Size: 920 568
StyI C'CwwG_G
Cuts at: 0 300 527 1211 1241 1488
Size: 300 227 684 30 247
Fragments arranged by size:
684 300 247 227 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1463 1488
Size: 1463 25
TatI w'GTAC_w
Cuts at: 0 1407 1488
Size: 1407 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 968 1000 1488
Size: 968 32 488
Fragments arranged by size:
968 488 32
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApoI AvaI BanI BanII
BbeI BbsI BciVI BfrBI BglI BglII BmrI BplI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstEII BstYI BtgI BtsI CspCI EaeI EarI Eco57MI
EcoO109I HaeII HincII KasI MscI MslI MspA1I NarI
NsiI NspI PciI PflMI PfoI PpuMI PsiI PsrI
PstI PvuII SapI SexAI SfcI SfoI StyI TaqII
TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BclI BlpI Bme1580I
BmgBI BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI
BsiEI BsiWI BsmBI BspEI BssHII BssSI BstBI BstXI
BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HindIII HpaI KpnI MfeI MluI MmeI
NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PmeI PmlI PpiI PshAI PspOMI PspXI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI