Restriction map for
pFN21AE6406
(Linear) (Six-base) MAPSORT of: KIEE6406.seq Check: 6966 from: 1 to: 1374
KIEE6406.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AclI AA'CG_TT
Cuts at: 0 172 1374
Size: 172 1202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 1214 1374
Size: 553 661 160
Fragments arranged by size:
661 553 160
AlwNI CAG_nnn'CTG
Cuts at: 0 436 1374
Size: 436 938
ApoI r'AATT_y
Cuts at: 0 43 111 232 280 426 466 508 922
Size: 43 68 121 48 146 40 42 414
Cuts at: 922 1216 1374
Size: 294 158
Fragments arranged by size:
414 294 158 146 121 68 48 43
42 40
BamHI G'GATC_C
Cuts at: 0 1032 1134 1374
Size: 1032 102 240
Fragments arranged by size:
1032 240 102
BbsI GAAGACnn'nnnn_
Cuts at: 0 1313 1374
Size: 1313 61
BclI T'GATC_A
Cuts at: 0 561 1374
Size: 561 813
BlpI GC'TnA_GC
Cuts at: 0 300 1374
Size: 300 1074
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 1105 1374
Size: 347 758 269
Fragments arranged by size:
758 347 269
BsaAI yAC'GTr
Cuts at: 0 210 1374
Size: 210 1164
BseYI C'CCAG_C
Cuts at: 0 1138 1374
Size: 1138 236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 1374
Size: 315 1059
BspHI T'CATG_A
Cuts at: 0 564 1374
Size: 564 810
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 186 1374
Size: 186 1188
BsrGI T'GTAC_A
Cuts at: 0 193 790 1144 1374
Size: 193 597 354 230
Fragments arranged by size:
597 354 230 193
BstBI TT'CG_AA
Cuts at: 0 1125 1374
Size: 1125 249
BstYI r'GATC_y
Cuts at: 0 1032 1134 1374
Size: 1032 102 240
Fragments arranged by size:
1032 240 102
BtsI GCAGTG_nn'
Cuts at: 0 1095 1374
Size: 1095 279
DraI TTT'AAA
Cuts at: 0 150 1374
Size: 150 1224
EaeI y'GGCC_r
Cuts at: 0 345 1374
Size: 345 1029
EarI CTCTTCn'nnn_
Cuts at: 0 1352 1374
Size: 1352 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 1214 1374
Size: 553 661 160
Fragments arranged by size:
661 553 160
EcoRI G'AATT_C
Cuts at: 0 280 508 1374
Size: 280 228 866
Fragments arranged by size:
866 280 228
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 508 1327 1374
Size: 508 819 47
Fragments arranged by size:
819 508 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 424 456 1270 1302 1374
Size: 424 32 814 32 72
Fragments arranged by size:
814 424 72 32 32
HincII GTy'rAC
Cuts at: 0 27 1374
Size: 27 1347
MfeI C'AATT_G
Cuts at: 0 542 1374
Size: 542 832
MscI TGG'CCA
Cuts at: 0 347 1374
Size: 347 1027
MslI CAynn'nnrTG
Cuts at: 0 1207 1374
Size: 1207 167
MspA1I CmG'CkG
Cuts at: 0 703 1142 1374
Size: 703 439 232
Fragments arranged by size:
703 439 232
PstI C_TGCA'G
Cuts at: 0 183 1374
Size: 183 1191
PvuII CAG'CTG
Cuts at: 0 703 1142 1374
Size: 703 439 232
Fragments arranged by size:
703 439 232
SfcI C'TryA_G
Cuts at: 0 8 179 270 1374
Size: 8 171 91 1104
Fragments arranged by size:
1104 171 91 8
SmlI C'TyrA_G
Cuts at: 0 362 1084 1374
Size: 362 722 290
Fragments arranged by size:
722 362 290
SspI AAT'ATT
Cuts at: 0 420 553 952 1374
Size: 420 133 399 422
Fragments arranged by size:
422 420 399 133
StyI C'CwwG_G
Cuts at: 0 623 1374
Size: 623 751
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 69 311 1374
Size: 69 242 1063
Fragments arranged by size:
1063 242 69
TatI w'GTAC_w
Cuts at: 0 193 790 1144 1374
Size: 193 597 354 230
Fragments arranged by size:
597 354 230 193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 689 721 1374
Size: 689 32 653
Fragments arranged by size:
689 653 32
XbaI T'CTAG_A
Cuts at: 0 253 1374
Size: 253 1121
XmnI GAAnn'nnTTC
Cuts at: 0 233 1374
Size: 233 1141
Enzymes that do cut:
AclI AcuI AlwNI ApoI BamHI BbsI BclI BlpI
BpuEI BsaAI BseYI BsgI BspHI BspMI BsrGI BstBI
BstYI BtsI DraI EaeI EarI Eco57MI EcoRI FalI
Hin4I HincII MfeI MscI MslI MspA1I PstI PvuII
SfcI SmlI SspI StyI TaqII TatI TstI XbaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BanII BbeI BbvCI
BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI Tth111I XcmI
XhoI XmaI ZraI