Restriction map for
pFN21AE6405
(Linear) (Six-base) MAPSORT of: KIEE6405.seq Check: 7150 from: 1 to: 1818
KIEE6405.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 316 1407 1533 1818
Size: 316 1091 126 285
Fragments arranged by size:
1091 316 285 126
AccI GT'mk_AC
Cuts at: 0 515 1442 1818
Size: 515 927 376
Fragments arranged by size:
927 515 376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 307 754 1087 1453 1818
Size: 103 204 447 333 366 365
Fragments arranged by size:
447 366 365 333 204 103
AflIII A'CryG_T
Cuts at: 0 1058 1818
Size: 1058 760
AlwNI CAG_nnn'CTG
Cuts at: 0 81 1248 1341 1459 1704 1818
Size: 81 1167 93 118 245 114
Fragments arranged by size:
1167 245 118 114 93 81
ApaI G_GGCC'C
Cuts at: 0 17 619 1358 1818
Size: 17 602 739 460
Fragments arranged by size:
739 602 460 17
AvaI C'yCGr_G
Cuts at: 0 553 1575 1818
Size: 553 1022 243
Fragments arranged by size:
1022 553 243
BanI G'GyrC_C
Cuts at: 0 31 1818
Size: 31 1787
BanII G_rGCy'C
Cuts at: 0 17 619 803 1330 1358 1685 1818
Size: 17 602 184 527 28 327 133
Fragments arranged by size:
602 527 327 184 133 28 17
BbeI G_GCGC'C
Cuts at: 0 35 1818
Size: 35 1783
BclI T'GATC_A
Cuts at: 0 297 1818
Size: 297 1521
BglII A'GATC_T
Cuts at: 0 48 1229 1818
Size: 48 1181 589
Fragments arranged by size:
1181 589 48
BlpI GC'TnA_GC
Cuts at: 0 469 916 1818
Size: 469 447 902
Fragments arranged by size:
902 469 447
Bme1580I G_kGCm'C
Cuts at: 0 17 619 1273 1358 1659 1818
Size: 17 602 654 85 301 159
Fragments arranged by size:
654 602 301 159 85 17
BmgBI CAC'GTC
Cuts at: 0 447 1818
Size: 447 1371
BmrI ACTGGGnnnn_n'
Cuts at: 0 185 1818
Size: 185 1633
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1191 1818
Size: 1191 627
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 175 351 673 1818
Size: 46 129 176 322 1145
Fragments arranged by size:
1145 322 176 129 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1186 1500 1765 1818
Size: 1186 314 265 53
Fragments arranged by size:
1186 314 265 53
BsaI GGTCTCn'nnnn_
Cuts at: 0 890 1179 1818
Size: 890 289 639
Fragments arranged by size:
890 639 289
BsaBI GATnn'nnATC
Cuts at: 0 1804 1818
Size: 1804 14
BsaHI Gr'CG_yC
Cuts at: 0 32 1818
Size: 32 1786
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 775 805 1818
Size: 775 30 1013
Fragments arranged by size:
1013 775 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1182 1796 1818
Size: 1182 614 22
Fragments arranged by size:
1182 614 22
BseYI C'CCAG_C
Cuts at: 0 721 974 1704 1818
Size: 721 253 730 114
Fragments arranged by size:
730 721 253 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1354 1818
Size: 1354 464
BsiEI CG_ry'CG
Cuts at: 0 8 1818
Size: 8 1810
BsiHKAI G_wGCw'C
Cuts at: 0 353 737 803 1685 1818
Size: 353 384 66 882 133
Fragments arranged by size:
882 384 353 133 66
BsmI GAATG_Cn'
Cuts at: 0 699 942 1818
Size: 699 243 876
Fragments arranged by size:
876 699 243
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1039 1818
Size: 1039 779
Bsp1286I G_dGCh'C
Cuts at: 0 17 353 619 737 803 1273 1330 1358
Size: 17 336 266 118 66 470 57 28
Cuts at: 1358 1659 1685 1818
Size: 301 26 133
Fragments arranged by size:
470 336 301 266 133 118 66 57
28 26 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 316 323 1407 1533 1625 1818
Size: 316 7 1084 126 92 193
Fragments arranged by size:
1084 316 193 126 92 7
BsrBI CCG'CTC
Cuts at: 0 1170 1818
Size: 1170 648
BsrFI r'CCGG_y
Cuts at: 0 461 1818
Size: 461 1357
BsrGI T'GTAC_A
Cuts at: 0 809 1818
Size: 809 1009
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1515 1818
Size: 1515 303
BstYI r'GATC_y
Cuts at: 0 48 1085 1215 1229 1475 1818
Size: 48 1037 130 14 246 343
Fragments arranged by size:
1037 343 246 130 48 14
BtgI C'CryG_G
Cuts at: 0 593 1359 1818
Size: 593 766 459
Fragments arranged by size:
766 593 459
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 849 1818
Size: 849 969
BtsI GCAGTG_nn'
Cuts at: 0 1640 1818
Size: 1640 178
ClaI AT'CG_AT
Cuts at: 0 416 1818
Size: 416 1402
EaeI y'GGCC_r
Cuts at: 0 479 1129 1695 1818
Size: 479 650 566 123
Fragments arranged by size:
650 566 479 123
EarI CTCTTCn'nnn_
Cuts at: 0 283 1615 1818
Size: 283 1332 203
Fragments arranged by size:
1332 283 203
EcoICRI GAG'CTC
Cuts at: 0 801 1683 1818
Size: 801 882 135
Fragments arranged by size:
882 801 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 103 175 307 351 673 754 1087
Size: 46 57 72 132 44 322 81 333
Cuts at: 1087 1453 1818
Size: 366 365
Fragments arranged by size:
366 365 333 322 132 81 72 57
46 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1210 1435 1663 1818
Size: 1210 225 228 155
Fragments arranged by size:
1210 228 225 155
EcoO109I rG'GnC_Cy
Cuts at: 0 13 207 339 616 1355 1403 1530 1818
Size: 13 194 132 277 739 48 127 288
Fragments arranged by size:
739 288 277 194 132 127 48 13
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 957 1125 1302 1407 1818
Size: 957 168 177 105 411
Fragments arranged by size:
957 411 177 168 105
HaeII r_GCGC'y
Cuts at: 0 35 1818
Size: 35 1783
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 177 209 282 314 775 807 1555 1587
Size: 177 32 73 32 461 32 748 32
Cuts at: 1587 1818
Size: 231
Fragments arranged by size:
748 461 231 177 73 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 31 1818
Size: 31 1787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1532 1818
Size: 1532 286
MscI TGG'CCA
Cuts at: 0 481 1131 1818
Size: 481 650 687
Fragments arranged by size:
687 650 481
MslI CAynn'nnrTG
Cuts at: 0 625 1723 1818
Size: 625 1098 95
Fragments arranged by size:
1098 625 95
MspA1I CmG'CkG
Cuts at: 0 66 884 965 1135 1704 1818
Size: 66 818 81 170 569 114
Fragments arranged by size:
818 569 170 114 81 66
NaeI GCC'GGC
Cuts at: 0 463 1818
Size: 463 1355
NarI GG'CG_CC
Cuts at: 0 32 1818
Size: 32 1786
NcoI C'CATG_G
Cuts at: 0 1359 1818
Size: 1359 459
NgoMIV G'CCGG_C
Cuts at: 0 461 1818
Size: 461 1357
NspI r_CATG'y
Cuts at: 0 681 730 1062 1818
Size: 681 49 332 756
Fragments arranged by size:
756 681 332 49
PasI CC'CwG_GG
Cuts at: 0 1123 1818
Size: 1123 695
PciI A'CATG_T
Cuts at: 0 1058 1818
Size: 1058 760
PflMI CCAn_nnn'nTGG
Cuts at: 0 447 1545 1704 1818
Size: 447 1098 159 114
Fragments arranged by size:
1098 447 159 114
PpuMI rG'GwC_Cy
Cuts at: 0 207 339 1403 1818
Size: 207 132 1064 415
Fragments arranged by size:
1064 415 207 132
PshAI GACnn'nnGTC
Cuts at: 0 902 1818
Size: 902 916
PspOMI G'GGCC_C
Cuts at: 0 13 615 1354 1818
Size: 13 602 739 464
Fragments arranged by size:
739 602 464 13
PstI C_TGCA'G
Cuts at: 0 77 761 1622 1818
Size: 77 684 861 196
Fragments arranged by size:
861 684 196 77
PvuII CAG'CTG
Cuts at: 0 965 1704 1818
Size: 965 739 114
Fragments arranged by size:
965 739 114
SacI G_AGCT'C
Cuts at: 0 803 1685 1818
Size: 803 882 133
Fragments arranged by size:
882 803 133
SanDI GG'GwC_CC
Cuts at: 0 1403 1818
Size: 1403 415
SapI GCTCTTCn'nnn_
Cuts at: 0 283 1818
Size: 283 1535
ScaI AGT'ACT
Cuts at: 0 199 1818
Size: 199 1619
SexAI A'CCwGG_T
Cuts at: 0 173 1818
Size: 173 1645
SfcI C'TryA_G
Cuts at: 0 73 714 757 1618 1818
Size: 73 641 43 861 200
Fragments arranged by size:
861 641 200 73 43
SfoI GGC'GCC
Cuts at: 0 33 1818
Size: 33 1785
SmaI CCC'GGG
Cuts at: 0 1577 1818
Size: 1577 241
SmlI C'TyrA_G
Cuts at: 0 1165 1479 1744 1818
Size: 1165 314 265 74
Fragments arranged by size:
1165 314 265 74
StuI AGG'CCT
Cuts at: 0 1502 1818
Size: 1502 316
StyI C'CwwG_G
Cuts at: 0 794 1359 1760 1818
Size: 794 565 401 58
Fragments arranged by size:
794 565 401 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 988 1291 1818
Size: 988 303 527
Fragments arranged by size:
988 527 303
TatI w'GTAC_w
Cuts at: 0 197 809 1318 1818
Size: 197 612 509 500
Fragments arranged by size:
612 509 500 197
XmaI C'CCGG_G
Cuts at: 0 1575 1818
Size: 1575 243
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApaI AvaI BanI
BanII BbeI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrFI BsrGI BstXI BstYI BtgI BtgZI BtsI
ClaI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PpuMI
PshAI PspOMI PstI PvuII SacI SanDI SapI ScaI
SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI BglI
BmtI Bpu10I BsaAI BsaWI BsiWI BspEI BspHI BsrDI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I CspCI
DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PfoI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII
SalI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI