Restriction map for
pFN21AE6401
(Linear) (Six-base) MAPSORT of: KIEE6401.seq Check: 7157 from: 1 to: 1431
KIEE6401.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AccI GT'mk_AC
Cuts at: 0 428 1431
Size: 428 1003
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1332 1431
Size: 1332 99
AflIII A'CryG_T
Cuts at: 0 356 1431
Size: 356 1075
AleI CACnn'nnGTG
Cuts at: 0 919 1431
Size: 919 512
AlwNI CAG_nnn'CTG
Cuts at: 0 804 909 1431
Size: 804 105 522
Fragments arranged by size:
804 522 105
ApaI G_GGCC'C
Cuts at: 0 109 1172 1431
Size: 109 1063 259
Fragments arranged by size:
1063 259 109
AvaI C'yCGr_G
Cuts at: 0 7 607 1431
Size: 7 600 824
Fragments arranged by size:
824 600 7
BanI G'GyrC_C
Cuts at: 0 235 641 1009 1068 1431
Size: 235 406 368 59 363
Fragments arranged by size:
406 368 363 235 59
BanII G_rGCy'C
Cuts at: 0 109 682 1172 1431
Size: 109 573 490 259
Fragments arranged by size:
573 490 259 109
BbeI G_GCGC'C
Cuts at: 0 645 1013 1072 1431
Size: 645 368 59 359
Fragments arranged by size:
645 368 359 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 69 704 1431
Size: 69 635 727
Fragments arranged by size:
727 635 69
BglI GCCn_nnn'nGGC
Cuts at: 0 520 1066 1117 1431
Size: 520 546 51 314
Fragments arranged by size:
546 520 314 51
BglII A'GATC_T
Cuts at: 0 1196 1431
Size: 1196 235
BlpI GC'TnA_GC
Cuts at: 0 292 716 1431
Size: 292 424 715
Fragments arranged by size:
715 424 292
Bme1580I G_kGCm'C
Cuts at: 0 109 317 572 1172 1431
Size: 109 208 255 600 259
Fragments arranged by size:
600 259 255 208 109
BmgBI CAC'GTC
Cuts at: 0 357 1431
Size: 357 1074
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1431
Size: 556 875
BsaI GGTCTCn'nnnn_
Cuts at: 0 604 832 1431
Size: 604 228 599
Fragments arranged by size:
604 599 228
BsaAI yAC'GTr
Cuts at: 0 1056 1431
Size: 1056 375
BsaHI Gr'CG_yC
Cuts at: 0 4 197 642 779 1010 1069 1431
Size: 4 193 445 137 231 59 362
Fragments arranged by size:
445 362 231 193 137 59 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 373 1038 1174 1431
Size: 373 665 136 257
Fragments arranged by size:
665 373 257 136
BseYI C'CCAG_C
Cuts at: 0 26 255 369 450 527 558 692 1163
Size: 26 229 114 81 77 31 134 471
Cuts at: 1163 1431
Size: 268
Fragments arranged by size:
471 268 229 134 114 81 77 31
26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1064 1431
Size: 502 562 367
Fragments arranged by size:
562 502 367
BsiEI CG_ry'CG
Cuts at: 0 69 887 1431
Size: 69 818 544
Fragments arranged by size:
818 544 69
BsiHKAI G_wGCw'C
Cuts at: 0 310 1431
Size: 310 1121
BsmBI CGTCTCn'nnnn_
Cuts at: 0 786 1431
Size: 786 645
Bsp1286I G_dGCh'C
Cuts at: 0 109 310 317 572 682 1172 1431
Size: 109 201 7 255 110 490 259
Fragments arranged by size:
490 259 255 201 110 109 7
BsrFI r'CCGG_y
Cuts at: 0 325 388 1147 1431
Size: 325 63 759 284
Fragments arranged by size:
759 325 284 63
BssHII G'CGCG_C
Cuts at: 0 56 165 1431
Size: 56 109 1266
Fragments arranged by size:
1266 109 56
BssSI C'ACGA_G
Cuts at: 0 996 1267 1431
Size: 996 271 164
Fragments arranged by size:
996 271 164
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1133 1431
Size: 1133 298
BstYI r'GATC_y
Cuts at: 0 1196 1431
Size: 1196 235
Bsu36I CC'TnA_GG
Cuts at: 0 1303 1431
Size: 1303 128
BtgI C'CryG_G
Cuts at: 0 63 239 344 653 1121 1431
Size: 63 176 105 309 468 310
Fragments arranged by size:
468 310 309 176 105 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1128 1431
Size: 1128 303
EaeI y'GGCC_r
Cuts at: 0 66 146 323 656 1058 1118 1431
Size: 66 80 177 333 402 60 313
Fragments arranged by size:
402 333 313 177 80 66 60
EagI C'GGCC_G
Cuts at: 0 66 1431
Size: 66 1365
EarI CTCTTCn'nnn_
Cuts at: 0 845 1144 1431
Size: 845 299 287
Fragments arranged by size:
845 299 287
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 966 1431
Size: 966 465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1332 1431
Size: 556 776 99
Fragments arranged by size:
776 556 99
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1294 1431
Size: 1294 137
EcoO109I rG'GnC_Cy
Cuts at: 0 12 40 1168 1299 1307 1431
Size: 12 28 1128 131 8 124
Fragments arranged by size:
1128 131 124 28 12 8
HaeII r_GCGC'y
Cuts at: 0 546 645 1013 1072 1431
Size: 546 99 368 59 359
Fragments arranged by size:
546 368 359 99 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1188 1220 1431
Size: 1188 32 211
Fragments arranged by size:
1188 211 32
HincII GTy'rAC
Cuts at: 0 816 1431
Size: 816 615
KasI G'GCGC_C
Cuts at: 0 641 1009 1068 1431
Size: 641 368 59 363
Fragments arranged by size:
641 368 363 59
MscI TGG'CCA
Cuts at: 0 1060 1431
Size: 1060 371
MslI CAynn'nnrTG
Cuts at: 0 904 919 1431
Size: 904 15 512
Fragments arranged by size:
904 512 15
MspA1I CmG'CkG
Cuts at: 0 65 97 241 346 804 919 1431
Size: 65 32 144 105 458 115 512
Fragments arranged by size:
512 458 144 115 105 65 32
NaeI GCC'GGC
Cuts at: 0 327 1149 1431
Size: 327 822 282
Fragments arranged by size:
822 327 282
NarI GG'CG_CC
Cuts at: 0 642 1010 1069 1431
Size: 642 368 59 362
Fragments arranged by size:
642 368 362 59
NcoI C'CATG_G
Cuts at: 0 653 1431
Size: 653 778
NgoMIV G'CCGG_C
Cuts at: 0 325 1147 1431
Size: 325 822 284
Fragments arranged by size:
822 325 284
NotI GC'GGCC_GC
Cuts at: 0 66 1431
Size: 66 1365
NspI r_CATG'y
Cuts at: 0 1134 1213 1431
Size: 1134 79 218
Fragments arranged by size:
1134 218 79
PasI CC'CwG_GG
Cuts at: 0 102 231 1029 1431
Size: 102 129 798 402
Fragments arranged by size:
798 402 129 102
PflMI CCAn_nnn'nTGG
Cuts at: 0 1178 1431
Size: 1178 253
PmlI CAC'GTG
Cuts at: 0 1056 1431
Size: 1056 375
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 844 876 1431
Size: 844 32 555
Fragments arranged by size:
844 555 32
PpuMI rG'GwC_Cy
Cuts at: 0 12 1299 1431
Size: 12 1287 132
Fragments arranged by size:
1287 132 12
PspOMI G'GGCC_C
Cuts at: 0 105 1168 1431
Size: 105 1063 263
Fragments arranged by size:
1063 263 105
PstI C_TGCA'G
Cuts at: 0 164 1431
Size: 164 1267
PvuII CAG'CTG
Cuts at: 0 804 919 1431
Size: 804 115 512
Fragments arranged by size:
804 512 115
SacII CC_GC'GG
Cuts at: 0 66 242 347 1431
Size: 66 176 105 1084
Fragments arranged by size:
1084 176 105 66
SfcI C'TryA_G
Cuts at: 0 135 160 1431
Size: 135 25 1271
Fragments arranged by size:
1271 135 25
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1117 1431
Size: 1117 314
SfoI GGC'GCC
Cuts at: 0 643 1011 1070 1431
Size: 643 368 59 361
Fragments arranged by size:
643 368 361 59
StyI C'CwwG_G
Cuts at: 0 653 1274 1431
Size: 653 621 157
Fragments arranged by size:
653 621 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 223 519 873 883 1431
Size: 223 296 354 10 548
Fragments arranged by size:
548 354 296 223 10
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1036 1104 1254 1431
Size: 1036 68 150 177
Fragments arranged by size:
1036 177 150 68
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BglI BglII BlpI Bme1580I BmgBI
BpmI BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BsrFI BssHII BssSI BstAPI BstYI
Bsu36I BtgI BtgZI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI
PflMI PmlI PpiI PpuMI PspOMI PstI PvuII SacII
SfcI SfiI SfoI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BmrI
BmtI BplI Bpu10I BpuEI BsaBI BsaWI BsaXI BsiWI
BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI BstBI
BstEII BstXI BstZ17I BtsI ClaI CspCI DraI DraIII
DrdI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI
NruI NsiI PacI PciI PfoI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI