Restriction map for
pFN21AE6395
(Linear) (Six-base) MAPSORT of: KIEE6395.seq Check: 3934 from: 1 to: 1239
KIEE6395.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AccI GT'mk_AC
Cuts at: 0 1106 1239
Size: 1106 133
Acc65I G'GTAC_C
Cuts at: 0 99 652 1239
Size: 99 553 587
Fragments arranged by size:
587 553 99
AclI AA'CG_TT
Cuts at: 0 1157 1239
Size: 1157 82
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 1239
Size: 865 374
AgeI A'CCGG_T
Cuts at: 0 1036 1239
Size: 1036 203
AleI CACnn'nnGTG
Cuts at: 0 439 1239
Size: 439 800
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 394 426 1239
Size: 394 32 813
Fragments arranged by size:
813 394 32
AvaI C'yCGr_G
Cuts at: 0 755 1239
Size: 755 484
BanI G'GyrC_C
Cuts at: 0 99 271 376 652 1115 1239
Size: 99 172 105 276 463 124
Fragments arranged by size:
463 276 172 124 105 99
BanII G_rGCy'C
Cuts at: 0 1033 1239
Size: 1033 206
BbvCI CC'TCA_GC
Cuts at: 0 482 1239
Size: 482 757
BclI T'GATC_A
Cuts at: 0 951 1239
Size: 951 288
Bme1580I G_kGCm'C
Cuts at: 0 792 1118 1239
Size: 792 326 121
Fragments arranged by size:
792 326 121
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1047 1239
Size: 1047 192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 1239
Size: 859 380
Bpu10I CC'TnA_GC
Cuts at: 0 482 550 1239
Size: 482 68 689
Fragments arranged by size:
689 482 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 334 1239
Size: 111 223 905
Fragments arranged by size:
905 223 111
BsaWI w'CCGG_w
Cuts at: 0 1036 1239
Size: 1036 203
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 335 365 1239
Size: 335 30 874
Fragments arranged by size:
874 335 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 627 809 1239
Size: 627 182 430
Fragments arranged by size:
627 430 182
BseYI C'CCAG_C
Cuts at: 0 217 785 1239
Size: 217 568 454
Fragments arranged by size:
568 454 217
BsiHKAI G_wGCw'C
Cuts at: 0 855 1239
Size: 855 384
BsmI GAATG_Cn'
Cuts at: 0 199 467 1239
Size: 199 268 772
Fragments arranged by size:
772 268 199
Bsp1286I G_dGCh'C
Cuts at: 0 792 855 1033 1118 1239
Size: 792 63 178 85 121
Fragments arranged by size:
792 178 121 85 63
BspHI T'CATG_A
Cuts at: 0 513 1239
Size: 513 726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 377 1239
Size: 377 862
BsrDI GCAATG_nn'
Cuts at: 0 1097 1239
Size: 1097 142
BsrFI r'CCGG_y
Cuts at: 0 1036 1239
Size: 1036 203
BssSI C'ACGA_G
Cuts at: 0 791 1239
Size: 791 448
BstAPI GCAn_nnn'nTGC
Cuts at: 0 194 1239
Size: 194 1045
BstEII G'GTnAC_C
Cuts at: 0 637 1239
Size: 637 602
BstYI r'GATC_y
Cuts at: 0 58 1065 1239
Size: 58 1007 174
Fragments arranged by size:
1007 174 58
BtgI C'CryG_G
Cuts at: 0 452 1134 1239
Size: 452 682 105
Fragments arranged by size:
682 452 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1031 1239
Size: 1031 208
BtsI GCAGTG_nn'
Cuts at: 0 981 1239
Size: 981 258
EaeI y'GGCC_r
Cuts at: 0 708 1239
Size: 708 531
EarI CTCTTCn'nnn_
Cuts at: 0 839 1239
Size: 839 400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 865 1239
Size: 859 6 374
Fragments arranged by size:
859 374 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 285 1239
Size: 285 954
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 114 146 1009 1041 1239
Size: 114 32 863 32 198
Fragments arranged by size:
863 198 114 32 32
HindIII A'AGCT_T
Cuts at: 0 445 1239
Size: 445 794
KpnI G_GTAC'C
Cuts at: 0 103 656 1239
Size: 103 553 583
Fragments arranged by size:
583 553 103
MfeI C'AATT_G
Cuts at: 0 1224 1239
Size: 1224 15
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 1239
Size: 1007 232
MslI CAynn'nnrTG
Cuts at: 0 439 1239
Size: 439 800
MspA1I CmG'CkG
Cuts at: 0 439 1239
Size: 439 800
NspI r_CATG'y
Cuts at: 0 490 1239
Size: 490 749
PasI CC'CwG_GG
Cuts at: 0 37 1239
Size: 37 1202
PflMI CCAn_nnn'nTGG
Cuts at: 0 1125 1239
Size: 1125 114
PshAI GACnn'nnGTC
Cuts at: 0 757 1239
Size: 757 482
PstI C_TGCA'G
Cuts at: 0 671 1239
Size: 671 568
PvuII CAG'CTG
Cuts at: 0 439 1239
Size: 439 800
SapI GCTCTTCn'nnn_
Cuts at: 0 839 1239
Size: 839 400
SfcI C'TryA_G
Cuts at: 0 459 667 1239
Size: 459 208 572
Fragments arranged by size:
572 459 208
SmaI CCC'GGG
Cuts at: 0 757 1239
Size: 757 482
SmlI C'TyrA_G
Cuts at: 0 90 313 1239
Size: 90 223 926
Fragments arranged by size:
926 223 90
StuI AGG'CCT
Cuts at: 0 738 1239
Size: 738 501
StyI C'CwwG_G
Cuts at: 0 815 1239
Size: 815 424
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 29 1113 1239
Size: 29 1084 126
Fragments arranged by size:
1084 126 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 67 99 131 567 599 1239
Size: 67 32 32 436 32 640
Fragments arranged by size:
640 436 67 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 529 1239
Size: 529 710
XmaI C'CCGG_G
Cuts at: 0 755 1239
Size: 755 484
Enzymes that do cut:
AccI Acc65I AclI AcuI AgeI AleI AloI AvaI
BanI BanII BbvCI BclI Bme1580I BplI BpmI Bpu10I
BpuEI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrFI BssSI BstAPI BstEII BstYI
BtgI BtgZI BtsI EaeI EarI Eco57MI EcoNI Hin4I
HindIII KpnI MfeI MmeI MslI MspA1I NspI PasI
PflMI PshAI PstI PvuII SapI SfcI SmaI SmlI
StuI StyI TaqII TstI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AflIII AhdI AlfI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BcgI BciVI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BsaI BsaAI BsaBI BsaHI
BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrGI BssHII
BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HpaI KasI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TatI XbaI XcmI XhoI XmnI ZraI