Restriction map for
pFN21AE6390
(Linear) (Six-base) MAPSORT of: KIEE6390.seq Check: 1077 from: 1 to: 1254
KIEE6390.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AfeI AGC'GCT
Cuts at: 0 1245 1254
Size: 1245 9
AlwNI CAG_nnn'CTG
Cuts at: 0 172 351 921 1254
Size: 172 179 570 333
Fragments arranged by size:
570 333 179 172
ApoI r'AATT_y
Cuts at: 0 10 53 954 1254
Size: 10 43 901 300
Fragments arranged by size:
901 300 43 10
BamHI G'GATC_C
Cuts at: 0 1068 1254
Size: 1068 186
BanI G'GyrC_C
Cuts at: 0 1054 1254
Size: 1054 200
BanII G_rGCy'C
Cuts at: 0 345 1254
Size: 345 909
BbeI G_GCGC'C
Cuts at: 0 1058 1254
Size: 1058 196
BbvCI CC'TCA_GC
Cuts at: 0 1155 1254
Size: 1155 99
BclI T'GATC_A
Cuts at: 0 362 449 1254
Size: 362 87 805
Fragments arranged by size:
805 362 87
BmrI ACTGGGnnnn_n'
Cuts at: 0 1003 1254
Size: 1003 251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 862 1254
Size: 305 557 392
Fragments arranged by size:
557 392 305
Bpu10I CC'TnA_GC
Cuts at: 0 1155 1254
Size: 1155 99
BsaI GGTCTCn'nnnn_
Cuts at: 0 559 1254
Size: 559 695
BsaHI Gr'CG_yC
Cuts at: 0 1055 1254
Size: 1055 199
BsaWI w'CCGG_w
Cuts at: 0 63 1254
Size: 63 1191
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1028 1254
Size: 1028 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 653 1254
Size: 59 594 601
Fragments arranged by size:
601 594 59
BsmI GAATG_Cn'
Cuts at: 0 1117 1254
Size: 1117 137
Bsp1286I G_dGCh'C
Cuts at: 0 345 1254
Size: 345 909
BspHI T'CATG_A
Cuts at: 0 395 725 1230 1254
Size: 395 330 505 24
Fragments arranged by size:
505 395 330 24
BsrDI GCAATG_nn'
Cuts at: 0 734 1254
Size: 734 520
BstYI r'GATC_y
Cuts at: 0 140 177 1068 1254
Size: 140 37 891 186
Fragments arranged by size:
891 186 140 37
BtgI C'CryG_G
Cuts at: 0 1049 1254
Size: 1049 205
EaeI y'GGCC_r
Cuts at: 0 659 1254
Size: 659 595
EarI CTCTTCn'nnn_
Cuts at: 0 121 853 1254
Size: 121 732 401
Fragments arranged by size:
732 401 121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 862 1254
Size: 305 557 392
Fragments arranged by size:
557 392 305
EcoO109I rG'GnC_Cy
Cuts at: 0 414 991 1254
Size: 414 577 263
Fragments arranged by size:
577 414 263
HaeII r_GCGC'y
Cuts at: 0 1058 1247 1254
Size: 1058 189 7
Fragments arranged by size:
1058 189 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 278 310 1254
Size: 278 32 944
Fragments arranged by size:
944 278 32
KasI G'GCGC_C
Cuts at: 0 1054 1254
Size: 1054 200
MfeI C'AATT_G
Cuts at: 0 248 1254
Size: 248 1006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 884 920 993 1254
Size: 285 599 36 73 261
Fragments arranged by size:
599 285 261 73 36
MscI TGG'CCA
Cuts at: 0 661 1254
Size: 661 593
MslI CAynn'nnrTG
Cuts at: 0 1099 1254
Size: 1099 155
MspA1I CmG'CkG
Cuts at: 0 1179 1254
Size: 1179 75
NarI GG'CG_CC
Cuts at: 0 1055 1254
Size: 1055 199
NcoI C'CATG_G
Cuts at: 0 1049 1254
Size: 1049 205
PflMI CCAn_nnn'nTGG
Cuts at: 0 151 575 657 1254
Size: 151 424 82 597
Fragments arranged by size:
597 424 151 82
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 77 109 1254
Size: 77 32 1145
Fragments arranged by size:
1145 77 32
PpuMI rG'GwC_Cy
Cuts at: 0 414 991 1254
Size: 414 577 263
Fragments arranged by size:
577 414 263
PshAI GACnn'nnGTC
Cuts at: 0 571 1254
Size: 571 683
PstI C_TGCA'G
Cuts at: 0 177 423 1254
Size: 177 246 831
Fragments arranged by size:
831 246 177
PvuII CAG'CTG
Cuts at: 0 1179 1254
Size: 1179 75
SanDI GG'GwC_CC
Cuts at: 0 414 1254
Size: 414 840
SapI GCTCTTCn'nnn_
Cuts at: 0 121 1254
Size: 121 1133
SfcI C'TryA_G
Cuts at: 0 173 419 505 740 1254
Size: 173 246 86 235 514
Fragments arranged by size:
514 246 235 173 86
SfoI GGC'GCC
Cuts at: 0 1056 1254
Size: 1056 198
StuI AGG'CCT
Cuts at: 0 583 1019 1254
Size: 583 436 235
Fragments arranged by size:
583 436 235
StyI C'CwwG_G
Cuts at: 0 774 1049 1254
Size: 774 275 205
Fragments arranged by size:
774 275 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 418 1254
Size: 418 836
TatI w'GTAC_w
Cuts at: 0 824 1254
Size: 824 430
Tth111I GACn'n_nGTC
Cuts at: 0 676 1254
Size: 676 578
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 352 1254
Size: 352 902
Enzymes that do cut:
AfeI AlwNI ApoI BamHI BanI BanII BbeI BbvCI
BclI BmrI BpmI Bpu10I BsaI BsaHI BsaWI BseRI
BsgI BsmI Bsp1286I BspHI BsrDI BstYI BtgI EaeI
EarI Eco57MI EcoO109I HaeII Hin4I KasI MfeI MmeI
MscI MslI MspA1I NarI NcoI PflMI PpiI PpuMI
PshAI PstI PvuII SanDI SapI SfcI SfoI StuI
StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BbsI BcgI BciVI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsaXI BseYI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TstI XbaI XhoI XmaI XmnI ZraI