Restriction map for
pFN21AE6388
(Linear) (Six-base) MAPSORT of: KIEE6388.seq Check: 2279 from: 1 to: 1131
KIEE6388.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AclI AA'CG_TT
Cuts at: 0 338 1131
Size: 338 793
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 445 1131
Size: 295 150 686
Fragments arranged by size:
686 295 150
AleI CACnn'nnGTG
Cuts at: 0 106 1131
Size: 106 1025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 832 1131
Size: 832 299
AlwNI CAG_nnn'CTG
Cuts at: 0 631 1131
Size: 631 500
ApoI r'AATT_y
Cuts at: 0 660 1131
Size: 660 471
AvaI C'yCGr_G
Cuts at: 0 318 1099 1131
Size: 318 781 32
Fragments arranged by size:
781 318 32
BanI G'GyrC_C
Cuts at: 0 325 1051 1131
Size: 325 726 80
Fragments arranged by size:
726 325 80
BanII G_rGCy'C
Cuts at: 0 95 233 792 1098 1131
Size: 95 138 559 306 33
Fragments arranged by size:
559 306 138 95 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 451 1131
Size: 451 680
BglI GCCn_nnn'nGGC
Cuts at: 0 323 1074 1131
Size: 323 751 57
Fragments arranged by size:
751 323 57
BlpI GC'TnA_GC
Cuts at: 0 24 839 1131
Size: 24 815 292
Fragments arranged by size:
815 292 24
Bme1580I G_kGCm'C
Cuts at: 0 328 1131
Size: 328 803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 109 474 805 806 1131
Size: 91 18 365 331 1 325
Fragments arranged by size:
365 331 325 91 18 1
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 1131
Size: 565 566
BsaI GGTCTCn'nnnn_
Cuts at: 0 183 1012 1131
Size: 183 829 119
Fragments arranged by size:
829 183 119
BsaBI GATnn'nnATC
Cuts at: 0 368 808 1131
Size: 368 440 323
Fragments arranged by size:
440 368 323
BsaWI w'CCGG_w
Cuts at: 0 164 1131
Size: 164 967
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 67 951 1131
Size: 64 3 884 180
Fragments arranged by size:
884 180 64 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 783 1131
Size: 783 348
BsiHKAI G_wGCw'C
Cuts at: 0 95 792 845 1131
Size: 95 697 53 286
Fragments arranged by size:
697 286 95 53
Bsp1286I G_dGCh'C
Cuts at: 0 95 233 328 792 845 1098 1131
Size: 95 138 95 464 53 253 33
Fragments arranged by size:
464 253 138 95 95 53 33
BspEI T'CCGG_A
Cuts at: 0 164 1131
Size: 164 967
BsrDI GCAATG_nn'
Cuts at: 0 200 1131
Size: 200 931
BstXI CCAn_nnnn'nTGG
Cuts at: 0 525 1131
Size: 525 606
BstYI r'GATC_y
Cuts at: 0 161 369 652 1131
Size: 161 208 283 479
Fragments arranged by size:
479 283 208 161
BtgI C'CryG_G
Cuts at: 0 155 401 1131
Size: 155 246 730
Fragments arranged by size:
730 246 155
BtsI GCAGTG_nn'
Cuts at: 0 833 1131
Size: 833 298
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 733 768 1131
Size: 733 35 363
Fragments arranged by size:
733 363 35
EaeI y'GGCC_r
Cuts at: 0 257 404 556 708 1110 1131
Size: 257 147 152 152 402 21
Fragments arranged by size:
402 257 152 152 147 21
EarI CTCTTCn'nnn_
Cuts at: 0 889 1131
Size: 889 242
EcoICRI GAG'CTC
Cuts at: 0 93 790 1131
Size: 93 697 341
Fragments arranged by size:
697 341 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 109 295 445 474 805 806 1131
Size: 91 18 186 150 29 331 1 325
Fragments arranged by size:
331 325 186 150 91 29 18 1
EcoO109I rG'GnC_Cy
Cuts at: 0 606 1131
Size: 606 525
EcoRI G'AATT_C
Cuts at: 0 660 1131
Size: 660 471
MfeI C'AATT_G
Cuts at: 0 9 1131
Size: 9 1122
MscI TGG'CCA
Cuts at: 0 259 558 710 1112 1131
Size: 259 299 152 402 19
Fragments arranged by size:
402 299 259 152 19
MslI CAynn'nnrTG
Cuts at: 0 106 118 205 382 523 814 1131
Size: 106 12 87 177 141 291 317
Fragments arranged by size:
317 291 177 141 106 87 12
MspA1I CmG'CkG
Cuts at: 0 891 1131
Size: 891 240
PasI CC'CwG_GG
Cuts at: 0 194 411 1131
Size: 194 217 720
Fragments arranged by size:
720 217 194
PfoI T'CCnGG_A
Cuts at: 0 451 1131
Size: 451 680
PpuMI rG'GwC_Cy
Cuts at: 0 606 1131
Size: 606 525
PvuII CAG'CTG
Cuts at: 0 891 1131
Size: 891 240
SacI G_AGCT'C
Cuts at: 0 95 792 1131
Size: 95 697 339
Fragments arranged by size:
697 339 95
SexAI A'CCwGG_T
Cuts at: 0 445 1131
Size: 445 686
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1074 1131
Size: 1074 57
SmaI CCC'GGG
Cuts at: 0 320 1131
Size: 320 811
SmlI C'TyrA_G
Cuts at: 0 580 1099 1131
Size: 580 519 32
Fragments arranged by size:
580 519 32
SspI AAT'ATT
Cuts at: 0 798 1131
Size: 798 333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 59 91 140 172 1131
Size: 59 32 49 32 959
Fragments arranged by size:
959 59 49 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 120 895 1029 1131
Size: 120 775 134 102
Fragments arranged by size:
775 134 120 102
XhoI C'TCGA_G
Cuts at: 0 1099 1131
Size: 1099 32
XmaI C'CCGG_G
Cuts at: 0 318 1131
Size: 318 813
Enzymes that do cut:
AclI AcuI AleI AlfI AlwNI ApoI AvaI BanI
BanII BbsI BglI BlpI Bme1580I BpmI BpuEI BsaI
BsaBI BsaWI BseRI BsgI BsiHKAI Bsp1286I BspEI BsrDI
BstXI BstYI BtgI BtsI CspCI EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRI MfeI MscI MslI MspA1I PasI
PfoI PpuMI PvuII SacI SexAI SfiI SmaI SmlI
SspI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaHI
BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI
EciI EcoNI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI StuI StyI
SwaI TaqII TatI Tth111I XbaI XmnI ZraI