Restriction map for
pFN21AE6387
(Linear) (Six-base) MAPSORT of: KIEE6387.seq Check: 4648 from: 1 to: 984
KIEE6387.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AatII G_ACGT'C
Cuts at: 0 344 984
Size: 344 640
AccI GT'mk_AC
Cuts at: 0 68 752 984
Size: 68 684 232
Fragments arranged by size:
684 232 68
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 941 984
Size: 487 454 43
Fragments arranged by size:
487 454 43
AflIII A'CryG_T
Cuts at: 0 279 293 984
Size: 279 14 691
Fragments arranged by size:
691 279 14
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 219 984
Size: 219 765
ApaI G_GGCC'C
Cuts at: 0 7 984
Size: 7 977
AseI AT'TA_AT
Cuts at: 0 785 984
Size: 785 199
BamHI G'GATC_C
Cuts at: 0 695 984
Size: 695 289
BanII G_rGCy'C
Cuts at: 0 7 984
Size: 7 977
BbsI GAAGACnn'nnnn_
Cuts at: 0 608 984
Size: 608 376
BfrBI ATG'CAT
Cuts at: 0 958 984
Size: 958 26
BglI GCCn_nnn'nGGC
Cuts at: 0 11 984
Size: 11 973
Bme1580I G_kGCm'C
Cuts at: 0 7 984
Size: 7 977
BmrI ACTGGGnnnn_n'
Cuts at: 0 401 984
Size: 401 583
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 184 984
Size: 184 800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 604 680 767 984
Size: 57 547 76 87 217
Fragments arranged by size:
547 217 87 76 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 350 984
Size: 255 95 634
Fragments arranged by size:
634 255 95
BsaAI yAC'GTr
Cuts at: 0 294 984
Size: 294 690
BsaHI Gr'CG_yC
Cuts at: 0 341 984
Size: 341 643
BsaWI w'CCGG_w
Cuts at: 0 451 984
Size: 451 533
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 954 984
Size: 954 30
BseYI C'CCAG_C
Cuts at: 0 39 716 944 984
Size: 39 677 228 40
Fragments arranged by size:
677 228 40 39
BsmI GAATG_Cn'
Cuts at: 0 960 984
Size: 960 24
Bsp1286I G_dGCh'C
Cuts at: 0 7 984
Size: 7 977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 114 984
Size: 114 870
BstEII G'GTnAC_C
Cuts at: 0 447 984
Size: 447 537
BstYI r'GATC_y
Cuts at: 0 44 695 984
Size: 44 651 289
Fragments arranged by size:
651 289 44
BstZ17I GTA'TAC
Cuts at: 0 69 984
Size: 69 915
BtsI GCAGTG_nn'
Cuts at: 0 201 984
Size: 201 783
ClaI AT'CG_AT
Cuts at: 0 253 984
Size: 253 731
EaeI y'GGCC_r
Cuts at: 0 90 984
Size: 90 894
EarI CTCTTCn'nnn_
Cuts at: 0 746 944 984
Size: 746 198 40
Fragments arranged by size:
746 198 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 487 604 680 767 941 984
Size: 57 430 117 76 87 174 43
Fragments arranged by size:
430 174 117 87 76 57 43
EcoO109I rG'GnC_Cy
Cuts at: 0 4 664 984
Size: 4 660 320
Fragments arranged by size:
660 320 4
HaeII r_GCGC'y
Cuts at: 0 114 984
Size: 114 870
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 954 984
Size: 954 30
MscI TGG'CCA
Cuts at: 0 92 984
Size: 92 892
MslI CAynn'nnrTG
Cuts at: 0 683 984
Size: 683 301
NsiI A_TGCA'T
Cuts at: 0 960 984
Size: 960 24
PasI CC'CwG_GG
Cuts at: 0 974 984
Size: 974 10
PfoI T'CCnGG_A
Cuts at: 0 744 984
Size: 744 240
PspOMI G'GGCC_C
Cuts at: 0 3 984
Size: 3 981
PstI C_TGCA'G
Cuts at: 0 108 984
Size: 108 876
ScaI AGT'ACT
Cuts at: 0 289 984
Size: 289 695
SfcI C'TryA_G
Cuts at: 0 104 914 984
Size: 104 810 70
Fragments arranged by size:
810 104 70
SmlI C'TyrA_G
Cuts at: 0 270 365 984
Size: 270 95 619
Fragments arranged by size:
619 270 95
StyI C'CwwG_G
Cuts at: 0 118 984
Size: 118 866
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 435 984
Size: 435 549
TatI w'GTAC_w
Cuts at: 0 287 984
Size: 287 697
XbaI T'CTAG_A
Cuts at: 0 813 984
Size: 813 171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 440 984
Size: 440 544
ZraI GAC'GTC
Cuts at: 0 342 984
Size: 342 642
Enzymes that do cut:
AatII AccI AcuI AflIII AlfI ApaI AseI BamHI
BanII BbsI BfrBI BglI Bme1580I BmrI BplI BpmI
BpuEI BsaAI BsaHI BsaWI BsaXI BseYI BsmI Bsp1286I
BspMI BstEII BstYI BstZ17I BtsI ClaI EaeI EarI
Eco57MI EcoO109I HaeII Hin4I MscI MslI NsiI PasI
PfoI PspOMI PstI ScaI SfcI SmlI StyI TaqII
TatI XbaI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI AlwNI ApaLI ApoI AscI AsiSI AvaI AvrII
BaeI BanI BbeI BbvCI BcgI BciVI BclI BglII
BlpI BmgBI BmtI Bpu10I BsaI BsaBI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI Bsu36I
BtgI BtgZI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XhoI XmaI XmnI