(Linear) (Six-base) MAPSORT of: KIEE6386.seq  Check: 6248  from: 1  to: 945



KIEE6386.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:30  ..

AccI GT'mk_AC

Cuts at:      0     353     945 
   Size:        353     592
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     649     945 
   Size:        649     296
 
AflIII A'CryG_T

Cuts at:      0     785     945 
   Size:        785     160
 
AlwNI CAG_nnn'CTG

Cuts at:      0     845     945 
   Size:        845     100
 
ApoI r'AATT_y

Cuts at:      0     382     945 
   Size:        382     563
 
AvaI C'yCGr_G

Cuts at:      0     820     945 
   Size:        820     125
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     297     945 
   Size:        297     648
 
BglI GCCn_nnn'nGGC

Cuts at:      0     479     945 
   Size:        479     466
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     304     945 
   Size:        304     641
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     497     651     945 
   Size:        365     132     154     294

  Fragments arranged by size: 

                 365     294     154     132
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     602     945 
   Size:        602     343
 
BsaAI yAC'GTr

Cuts at:      0     788     945 
   Size:        788     157
 
BsaBI GATnn'nnATC

Cuts at:      0     734     945 
   Size:        734     211
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     470     500     945 
   Size:        470      30     445

  Fragments arranged by size: 

                 470     445      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     927     945 
   Size:        927      18
 
BseYI C'CCAG_C

Cuts at:      0     577     589     945 
   Size:        577      12     356

  Fragments arranged by size: 

                 577     356      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     671     945 
   Size:        671     274
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     365     945 
   Size:        365     580
 
BsrGI T'GTAC_A

Cuts at:      0     611     945 
   Size:        611     334
 
BstEII G'GTnAC_C

Cuts at:      0     676     945 
   Size:        676     269
 
BstZ17I GTA'TAC

Cuts at:      0     354     945 
   Size:        354     591
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     497     649     651     945 
   Size:        365     132     152       2     294

  Fragments arranged by size: 

                 365     294     152     132       2
 
EcoO109I rG'GnC_Cy

Cuts at:      0     622     945 
   Size:        622     323
 
EcoRV GAT'ATC

Cuts at:      0     724     945 
   Size:        724     221
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     470     502     945 
   Size:        470      32     443

  Fragments arranged by size: 

                 470     443      32
 
HincII GTy'rAC

Cuts at:      0     489     945 
   Size:        489     456
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     582     945 
   Size:        582     363
 
MslI CAynn'nnrTG

Cuts at:      0     227     945 
   Size:        227     718
 
MspA1I CmG'CkG

Cuts at:      0     921     945 
   Size:        921      24
 
NspI r_CATG'y

Cuts at:      0     650     945 
   Size:        650     295
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     266     845     945 
   Size:        266     579     100

  Fragments arranged by size: 

                 579     266     100
 
SfcI C'TryA_G

Cuts at:      0     419     945 
   Size:        419     526
 
SmlI C'TyrA_G

Cuts at:      0     617     820     945 
   Size:        617     203     125

  Fragments arranged by size: 

                 617     203     125
 
SspI AAT'ATT

Cuts at:      0      78     945 
   Size:         78     867
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     786     866     945 
   Size:        786      80      79

  Fragments arranged by size: 

                 786      80      79
 
TatI w'GTAC_w

Cuts at:      0     336     611     945 
   Size:        336     275     334

  Fragments arranged by size: 

                 336     334     275
 
XbaI T'CTAG_A

Cuts at:      0     282     945 
   Size:        282     663
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     472     945 
   Size:        472     473
 
XhoI C'TCGA_G

Cuts at:      0     820     945 
   Size:        820     125
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApoI     AvaI     BbsI     BglI
     BmrI     BpmI    BpuEI    BsaAI    BsaBI    BsaXI    BseRI    BseYI
     BsgI    BspMI    BsrGI   BstEII  BstZ17I  Eco57MI EcoO109I    EcoRV
    Hin4I   HincII     MmeI     MslI   MspA1I     NspI    PflMI     SfcI
     SmlI     SspI    TaqII     TatI     XbaI     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I     BsaI    BsaHI    BsaWI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI     FalI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
     TstI  Tth111I     XmaI     XmnI     ZraI