Restriction map for
pFN21AE6385
(Linear) (Six-base) MAPSORT of: KIEE6385.seq Check: 7457 from: 1 to: 1020
KIEE6385.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 483 1020
Size: 483 537
AatII G_ACGT'C
Cuts at: 0 106 177 1020
Size: 106 71 843
Fragments arranged by size:
843 106 71
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 1020
Size: 105 915
AlwNI CAG_nnn'CTG
Cuts at: 0 198 387 414 1020
Size: 198 189 27 606
Fragments arranged by size:
606 198 189 27
ApaLI G'TGCA_C
Cuts at: 0 257 1020
Size: 257 763
AvaI C'yCGr_G
Cuts at: 0 54 228 1020
Size: 54 174 792
Fragments arranged by size:
792 174 54
BanII G_rGCy'C
Cuts at: 0 718 1020
Size: 718 302
BbsI GAAGACnn'nnnn_
Cuts at: 0 358 393 832 1020
Size: 358 35 439 188
Fragments arranged by size:
439 358 188 35
BciVI GTATCCnnnnn_n'
Cuts at: 0 490 1020
Size: 490 530
BglII A'GATC_T
Cuts at: 0 305 1020
Size: 305 715
Bme1580I G_kGCm'C
Cuts at: 0 261 1020
Size: 261 759
BmrI ACTGGGnnnn_n'
Cuts at: 0 88 1020
Size: 88 932
BmtI G_CTAG'C
Cuts at: 0 50 1020
Size: 50 970
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 273 691 769 841 1020
Size: 220 53 418 78 72 179
Fragments arranged by size:
418 220 179 78 72 53
BsaHI Gr'CG_yC
Cuts at: 0 103 174 349 1020
Size: 103 71 175 671
Fragments arranged by size:
671 175 103 71
BsaWI w'CCGG_w
Cuts at: 0 288 1020
Size: 288 732
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 708 850 1020
Size: 708 142 170
Fragments arranged by size:
708 170 142
BseYI C'CCAG_C
Cuts at: 0 576 1020
Size: 576 444
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 1020
Size: 211 809
BsiEI CG_ry'CG
Cuts at: 0 806 1020
Size: 806 214
BsiHKAI G_wGCw'C
Cuts at: 0 261 1020
Size: 261 759
BsmBI CGTCTCn'nnnn_
Cuts at: 0 165 181 528 745 1020
Size: 165 16 347 217 275
Fragments arranged by size:
347 275 217 165 16
Bsp1286I G_dGCh'C
Cuts at: 0 261 718 1020
Size: 261 457 302
Fragments arranged by size:
457 302 261
BspEI T'CCGG_A
Cuts at: 0 288 1020
Size: 288 732
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 483 1020
Size: 483 537
BsrDI GCAATG_nn'
Cuts at: 0 462 527 648 1020
Size: 462 65 121 372
Fragments arranged by size:
462 372 121 65
BsrGI T'GTAC_A
Cuts at: 0 1004 1020
Size: 1004 16
BssSI C'ACGA_G
Cuts at: 0 709 877 984 1020
Size: 709 168 107 36
Fragments arranged by size:
709 168 107 36
BstEII G'GTnAC_C
Cuts at: 0 234 1020
Size: 234 786
BstXI CCAn_nnnn'nTGG
Cuts at: 0 534 1020
Size: 534 486
BstYI r'GATC_y
Cuts at: 0 305 1020
Size: 305 715
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 74 713 1020
Size: 74 639 307
Fragments arranged by size:
639 307 74
BtsI GCAGTG_nn'
Cuts at: 0 415 1020
Size: 415 605
EaeI y'GGCC_r
Cuts at: 0 114 914 1020
Size: 114 800 106
Fragments arranged by size:
800 114 106
EarI CTCTTCn'nnn_
Cuts at: 0 931 1020
Size: 931 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1020
Size: 19 1001
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 220 273 691 769 841 1020
Size: 105 115 53 418 78 72 179
Fragments arranged by size:
418 179 115 105 78 72 53
EcoO109I rG'GnC_Cy
Cuts at: 0 218 1020
Size: 218 802
EcoRV GAT'ATC
Cuts at: 0 138 1020
Size: 138 882
HaeII r_GCGC'y
Cuts at: 0 53 521 760 1020
Size: 53 468 239 260
Fragments arranged by size:
468 260 239 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 497 529 1020
Size: 497 32 491
Fragments arranged by size:
497 491 32
HincII GTy'rAC
Cuts at: 0 441 1020
Size: 441 579
MfeI C'AATT_G
Cuts at: 0 353 1020
Size: 353 667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 1020
Size: 99 921
MscI TGG'CCA
Cuts at: 0 916 1020
Size: 916 104
MslI CAynn'nnrTG
Cuts at: 0 532 790 814 1020
Size: 532 258 24 206
Fragments arranged by size:
532 258 206 24
MspA1I CmG'CkG
Cuts at: 0 195 912 1020
Size: 195 717 108
Fragments arranged by size:
717 195 108
NheI G'CTAG_C
Cuts at: 0 46 1020
Size: 46 974
PflMI CCAn_nnn'nTGG
Cuts at: 0 387 1020
Size: 387 633
PfoI T'CCnGG_A
Cuts at: 0 689 1020
Size: 689 331
PpuMI rG'GwC_Cy
Cuts at: 0 218 1020
Size: 218 802
PspXI vC'TCGA_Gb
Cuts at: 0 54 1020
Size: 54 966
PstI C_TGCA'G
Cuts at: 0 842 1020
Size: 842 178
PvuI CG_AT'CG
Cuts at: 0 806 1020
Size: 806 214
PvuII CAG'CTG
Cuts at: 0 195 1020
Size: 195 825
SanDI GG'GwC_CC
Cuts at: 0 218 1020
Size: 218 802
SbfI CC_TGCA'GG
Cuts at: 0 842 1020
Size: 842 178
SfcI C'TryA_G
Cuts at: 0 17 620 838 1020
Size: 17 603 218 182
Fragments arranged by size:
603 218 182 17
SmaI CCC'GGG
Cuts at: 0 230 1020
Size: 230 790
SmlI C'TyrA_G
Cuts at: 0 54 1020
Size: 54 966
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 352 1020
Size: 352 668
TatI w'GTAC_w
Cuts at: 0 1004 1020
Size: 1004 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 56 88 1020
Size: 56 32 932
Fragments arranged by size:
932 56 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 384 786 1020
Size: 384 402 234
Fragments arranged by size:
402 384 234
XhoI C'TCGA_G
Cuts at: 0 54 1020
Size: 54 966
XmaI C'CCGG_G
Cuts at: 0 228 1020
Size: 228 792
XmnI GAAnn'nnTTC
Cuts at: 0 307 1020
Size: 307 713
ZraI GAC'GTC
Cuts at: 0 104 175 1020
Size: 104 71 845
Fragments arranged by size:
845 104 71
Enzymes that do cut:
AarI AatII AcuI AlwNI ApaLI AvaI BanII BbsI
BciVI BglII Bme1580I BmrI BmtI BpmI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrDI BsrGI BssSI BstEII BstXI BstYI BtgZI
BtsI EaeI EarI EciI Eco57MI EcoO109I EcoRV HaeII
Hin4I HincII MfeI MmeI MscI MslI MspA1I NheI
PflMI PfoI PpuMI PspXI PstI PvuI PvuII SanDI
SbfI SfcI SmaI SmlI TaqII TatI TstI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BclI
BfrBI BglI BlpI BmgBI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaXI BsiWI BsmI BspHI BsrBI BsrFI
BssHII BstAPI BstBI BstZ17I Bsu36I BtgI ClaI CspCI
DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI FalI
FseI FspI FspAI HindIII HpaI KasI KpnI MluI
NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI Tth111I XbaI