Restriction map for
pFN21AE6384
(Linear) (Six-base) MAPSORT of: KIEE6384.seq Check: 1668 from: 1 to: 993
KIEE6384.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 411 993
Size: 411 582
AflIII A'CryG_T
Cuts at: 0 752 993
Size: 752 241
AleI CACnn'nnGTG
Cuts at: 0 934 993
Size: 934 59
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 57 993
Size: 57 936
ApoI r'AATT_y
Cuts at: 0 683 829 993
Size: 683 146 164
Fragments arranged by size:
683 164 146
AvrII C'CTAG_G
Cuts at: 0 948 993
Size: 948 45
BanI G'GyrC_C
Cuts at: 0 895 993
Size: 895 98
BanII G_rGCy'C
Cuts at: 0 68 993
Size: 68 925
BbsI GAAGACnn'nnnn_
Cuts at: 0 615 900 993
Size: 615 285 93
Fragments arranged by size:
615 285 93
Bme1580I G_kGCm'C
Cuts at: 0 898 993
Size: 898 95
BmrI ACTGGGnnnn_n'
Cuts at: 0 426 993
Size: 426 567
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 993
Size: 462 531
BsaAI yAC'GTr
Cuts at: 0 753 993
Size: 753 240
BsaWI w'CCGG_w
Cuts at: 0 292 993
Size: 292 701
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 571 601 993
Size: 571 30 392
Fragments arranged by size:
571 392 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 718 993
Size: 718 275
BseYI C'CCAG_C
Cuts at: 0 891 993
Size: 891 102
BsiHKAI G_wGCw'C
Cuts at: 0 68 993
Size: 68 925
BsmI GAATG_Cn'
Cuts at: 0 793 993
Size: 793 200
Bsp1286I G_dGCh'C
Cuts at: 0 68 898 993
Size: 68 830 95
Fragments arranged by size:
830 95 68
BspHI T'CATG_A
Cuts at: 0 731 993
Size: 731 262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 411 993
Size: 411 582
BsrDI GCAATG_nn'
Cuts at: 0 260 368 993
Size: 260 108 625
Fragments arranged by size:
625 260 108
BsrGI T'GTAC_A
Cuts at: 0 926 993
Size: 926 67
BstEII G'GTnAC_C
Cuts at: 0 207 993
Size: 207 786
BstXI CCAn_nnnn'nTGG
Cuts at: 0 375 993
Size: 375 618
BstYI r'GATC_y
Cuts at: 0 289 993
Size: 289 704
BtgI C'CryG_G
Cuts at: 0 782 993
Size: 782 211
DraIII CAC_nnn'GTG
Cuts at: 0 881 993
Size: 881 112
DrdI GACnn_nn'nnGTC
Cuts at: 0 432 993
Size: 432 561
EarI CTCTTCn'nnn_
Cuts at: 0 515 993
Size: 515 478
EcoICRI GAG'CTC
Cuts at: 0 66 993
Size: 66 927
EcoO109I rG'GnC_Cy
Cuts at: 0 917 993
Size: 917 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 492 524 993
Size: 492 32 469
Fragments arranged by size:
492 469 32
MslI CAynn'nnrTG
Cuts at: 0 205 265 406 471 697 934 993
Size: 205 60 141 65 226 237 59
Fragments arranged by size:
237 226 205 141 65 60 59
NdeI CA'TA_TG
Cuts at: 0 278 993
Size: 278 715
PflMI CCAn_nnn'nTGG
Cuts at: 0 91 716 993
Size: 91 625 277
Fragments arranged by size:
625 277 91
PfoI T'CCnGG_A
Cuts at: 0 84 993
Size: 84 909
PpuMI rG'GwC_Cy
Cuts at: 0 917 993
Size: 917 76
PsiI TTA'TAA
Cuts at: 0 593 993
Size: 593 400
PstI C_TGCA'G
Cuts at: 0 46 993
Size: 46 947
SacI G_AGCT'C
Cuts at: 0 68 993
Size: 68 925
SapI GCTCTTCn'nnn_
Cuts at: 0 515 993
Size: 515 478
SfcI C'TryA_G
Cuts at: 0 42 993
Size: 42 951
SmlI C'TyrA_G
Cuts at: 0 441 993
Size: 441 552
StyI C'CwwG_G
Cuts at: 0 11 575 948 993
Size: 11 564 373 45
Fragments arranged by size:
564 373 45 11
TatI w'GTAC_w
Cuts at: 0 857 926 993
Size: 857 69 67
Fragments arranged by size:
857 69 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 164 196 993
Size: 164 32 797
Fragments arranged by size:
797 164 32
XmnI GAAnn'nnTTC
Cuts at: 0 871 993
Size: 871 122
Enzymes that do cut:
AarI AflIII AleI AlfI ApoI AvrII BanI BanII
BbsI Bme1580I BmrI BpuEI BsaAI BsaWI BsaXI BseRI
BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI
BstEII BstXI BstYI BtgI DraIII DrdI EarI EcoICRI
EcoO109I Hin4I MslI NdeI PflMI PfoI PpuMI PsiI
PstI SacI SapI SfcI SmlI StyI TatI TstI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BplI BpmI
Bpu10I BsaI BsaBI BsaHI BsgI BsiEI BsiWI BsmBI
BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI EaeI EagI
EciI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI ZraI