Restriction map for
pFN21AE6382
(Linear) (Six-base) MAPSORT of: KIEE6382.seq Check: 8565 from: 1 to: 981
KIEE6382.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AccI GT'mk_AC
Cuts at: 0 705 833 981
Size: 705 128 148
Fragments arranged by size:
705 148 128
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 673 981
Size: 319 354 308
Fragments arranged by size:
354 319 308
AflIII A'CryG_T
Cuts at: 0 251 981
Size: 251 730
AleI CACnn'nnGTG
Cuts at: 0 397 981
Size: 397 584
AlwNI CAG_nnn'CTG
Cuts at: 0 246 297 981
Size: 246 51 684
Fragments arranged by size:
684 246 51
ApoI r'AATT_y
Cuts at: 0 590 981
Size: 590 391
BamHI G'GATC_C
Cuts at: 0 912 981
Size: 912 69
BanII G_rGCy'C
Cuts at: 0 59 981
Size: 59 922
BclI T'GATC_A
Cuts at: 0 479 981
Size: 479 502
BglII A'GATC_T
Cuts at: 0 895 981
Size: 895 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 73 510 981
Size: 58 15 437 471
Fragments arranged by size:
471 437 58 15
Bpu10I CC'TnA_GC
Cuts at: 0 452 981
Size: 452 529
BsaI GGTCTCn'nnnn_
Cuts at: 0 25 981
Size: 25 956
BsaBI GATnn'nnATC
Cuts at: 0 900 981
Size: 900 81
BseYI C'CCAG_C
Cuts at: 0 573 981
Size: 573 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 981
Size: 64 917
BsiHKAI G_wGCw'C
Cuts at: 0 59 981
Size: 59 922
BsmI GAATG_Cn'
Cuts at: 0 758 981
Size: 758 223
Bsp1286I G_dGCh'C
Cuts at: 0 59 981
Size: 59 922
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 377 981
Size: 377 604
BsrDI GCAATG_nn'
Cuts at: 0 964 981
Size: 964 17
BsrFI r'CCGG_y
Cuts at: 0 16 233 981
Size: 16 217 748
Fragments arranged by size:
748 217 16
BsrGI T'GTAC_A
Cuts at: 0 629 981
Size: 629 352
BstAPI GCAn_nnn'nTGC
Cuts at: 0 966 981
Size: 966 15
BstYI r'GATC_y
Cuts at: 0 447 728 895 912 981
Size: 447 281 167 17 69
Fragments arranged by size:
447 281 167 69 17
BtgI C'CryG_G
Cuts at: 0 610 981
Size: 610 371
BtsI GCAGTG_nn'
Cuts at: 0 790 981
Size: 790 191
EaeI y'GGCC_r
Cuts at: 0 323 981
Size: 323 658
EarI CTCTTCn'nnn_
Cuts at: 0 886 981
Size: 886 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 196 981
Size: 196 785
EcoICRI GAG'CTC
Cuts at: 0 57 981
Size: 57 924
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 73 319 510 673 981
Size: 58 15 246 191 163 308
Fragments arranged by size:
308 246 191 163 58 15
EcoO109I rG'GnC_Cy
Cuts at: 0 318 981
Size: 318 663
EcoRI G'AATT_C
Cuts at: 0 590 981
Size: 590 391
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 324 981
Size: 324 657
FspI TGC'GCA
Cuts at: 0 142 981
Size: 142 839
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 432 464 981
Size: 432 32 517
Fragments arranged by size:
517 432 32
HindIII A'AGCT_T
Cuts at: 0 261 981
Size: 261 720
MfeI C'AATT_G
Cuts at: 0 372 981
Size: 372 609
MscI TGG'CCA
Cuts at: 0 325 981
Size: 325 656
MslI CAynn'nnrTG
Cuts at: 0 397 806 850 981
Size: 397 409 44 131
Fragments arranged by size:
409 397 131 44
NcoI C'CATG_G
Cuts at: 0 610 981
Size: 610 371
NspI r_CATG'y
Cuts at: 0 255 849 960 981
Size: 255 594 111 21
Fragments arranged by size:
594 255 111 21
PasI CC'CwG_GG
Cuts at: 0 313 981
Size: 313 668
PciI A'CATG_T
Cuts at: 0 251 981
Size: 251 730
PfoI T'CCnGG_A
Cuts at: 0 92 981
Size: 92 889
PstI C_TGCA'G
Cuts at: 0 563 981
Size: 563 418
SacI G_AGCT'C
Cuts at: 0 59 981
Size: 59 922
SbfI CC_TGCA'GG
Cuts at: 0 563 981
Size: 563 418
ScaI AGT'ACT
Cuts at: 0 223 923 981
Size: 223 700 58
Fragments arranged by size:
700 223 58
SfcI C'TryA_G
Cuts at: 0 559 840 917 981
Size: 559 281 77 64
Fragments arranged by size:
559 281 77 64
SphI G_CATG'C
Cuts at: 0 849 981
Size: 849 132
StuI AGG'CCT
Cuts at: 0 202 557 981
Size: 202 355 424
Fragments arranged by size:
424 355 202
StyI C'CwwG_G
Cuts at: 0 610 981
Size: 610 371
TatI w'GTAC_w
Cuts at: 0 221 629 824 921 981
Size: 221 408 195 97 60
Fragments arranged by size:
408 221 195 97 60
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApoI BamHI BanII
BclI BglII BpmI Bpu10I BsaI BsaBI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI
BstYI BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI Hin4I HindIII MfeI MscI
MslI NcoI NspI PasI PciI PfoI PstI SacI
SbfI ScaI SfcI SphI StuI StyI TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoNI EcoRV FseI FspAI HaeII HincII
HpaI KasI KpnI MluI MmeI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI