(Linear) (Six-base) MAPSORT of: KIEE6379.seq  Check: 9070  from: 1  to: 981



KIEE6379.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:30  ..

AccI GT'mk_AC

Cuts at:      0     519     981 
   Size:        519     462
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     981 
   Size:        113     868
 
ApaLI G'TGCA_C

Cuts at:      0     713     981 
   Size:        713     268
 
ApoI r'AATT_y

Cuts at:      0     211     259     328     529     581     799     822     981 
   Size:        211      48      69     201      52     218      23     159

  Fragments arranged by size: 

                 218     211     201     159      69      52      48      23
            
 
BanI G'GyrC_C

Cuts at:      0     367     981 
   Size:        367     614
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     420     851     981 
   Size:        420     431     130

  Fragments arranged by size: 

                 431     420     130
 
BclI T'GATC_A

Cuts at:      0     632     981 
   Size:        632     349
 
Bme1580I G_kGCm'C

Cuts at:      0     717     981 
   Size:        717     264
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     656     981 
   Size:        656     325
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     269     908     981 
   Size:        269     639      73

  Fragments arranged by size: 

                 639     269      73
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     929     981 
   Size:        314     615      52

  Fragments arranged by size: 

                 615     314      52
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     402     981 
   Size:        402     579
 
BsaBI GATnn'nnATC

Cuts at:      0     939     981 
   Size:        939      42
 
BsaWI w'CCGG_w

Cuts at:      0     848     981 
   Size:        848     133
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     125     155     981 
   Size:        125      30     826

  Fragments arranged by size: 

                 826     125      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     717     981 
   Size:        717     264
 
Bsp1286I G_dGCh'C

Cuts at:      0     717     981 
   Size:        717     264
 
BspHI T'CATG_A

Cuts at:      0     255     981 
   Size:        255     726
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      75     981 
   Size:         75     906
 
ClaI AT'CG_AT

Cuts at:      0      41     981 
   Size:         41     940
 
DraI TTT'AAA

Cuts at:      0     560     981 
   Size:        560     421
 
EarI CTCTTCn'nnn_

Cuts at:      0     127     981 
   Size:        127     854
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     314     929     981 
   Size:        113     201     615      52

  Fragments arranged by size: 

                 615     201     113      52
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     121     153     981 
   Size:        121      32     828

  Fragments arranged by size: 

                 828     121      32
 
HincII GTy'rAC

Cuts at:      0     480     981 
   Size:        480     501
 
HpaI GTT'AAC

Cuts at:      0     480     981 
   Size:        480     501
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     555     799     981 
   Size:        555     244     182

  Fragments arranged by size: 

                 555     244     182
 
MspA1I CmG'CkG

Cuts at:      0     630     981 
   Size:        630     351
 
NdeI CA'TA_TG

Cuts at:      0     351     617     981 
   Size:        351     266     364

  Fragments arranged by size: 

                 364     351     266
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     272     304     981 
   Size:        272      32     677

  Fragments arranged by size: 

                 677     272      32
 
PvuII CAG'CTG

Cuts at:      0     630     981 
   Size:        630     351
 
SmlI C'TyrA_G

Cuts at:      0     381     981 
   Size:        381     600
 
StyI C'CwwG_G

Cuts at:      0     195     981 
   Size:        195     786
 
XmnI GAAnn'nnTTC

Cuts at:      0     734     981 
   Size:        734     247
 

 Enzymes that do cut:

     AccI     AcuI    ApaLI     ApoI     BanI     BbsI     BclI Bme1580I
     BmrI     BplI     BpmI    BpuEI    BsaBI    BsaWI    BsaXI  BsiHKAI
 Bsp1286I    BspHI    BstXI     ClaI     DraI     EarI  Eco57MI    Hin4I
   HincII     HpaI     MmeI   MspA1I     NdeI     PsrI    PvuII     SmlI
     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
   Bpu10I     BsaI    BsaAI    BsaHI    BseRI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI