Restriction map for
pFN21AE6363
(Linear) (Six-base) MAPSORT of: KIEE6363.seq Check: 2879 from: 1 to: 1272
KIEE6363.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AccI GT'mk_AC
Cuts at: 0 52 1272
Size: 52 1220
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 659 781 838 991 1041 1272
Size: 445 214 122 57 153 50 231
Fragments arranged by size:
445 231 214 153 122 57 50
AflIII A'CryG_T
Cuts at: 0 986 1272
Size: 986 286
AleI CACnn'nnGTG
Cuts at: 0 1033 1272
Size: 1033 239
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 973 1005 1272
Size: 973 32 267
Fragments arranged by size:
973 267 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1039 1272
Size: 1039 233
ApoI r'AATT_y
Cuts at: 0 243 268 381 1072 1272
Size: 243 25 113 691 200
Fragments arranged by size:
691 243 200 113 25
AvrII C'CTAG_G
Cuts at: 0 1153 1272
Size: 1153 119
BamHI G'GATC_C
Cuts at: 0 443 1166 1272
Size: 443 723 106
Fragments arranged by size:
723 443 106
BanI G'GyrC_C
Cuts at: 0 67 1272
Size: 67 1205
BbsI GAAGACnn'nnnn_
Cuts at: 0 324 1272
Size: 324 948
BclI T'GATC_A
Cuts at: 0 870 1272
Size: 870 402
BglII A'GATC_T
Cuts at: 0 1173 1272
Size: 1173 99
BmgBI CAC'GTC
Cuts at: 0 532 1272
Size: 532 740
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 1118 1272
Size: 75 1043 154
Fragments arranged by size:
1043 154 75
BsaHI Gr'CG_yC
Cuts at: 0 432 1272
Size: 432 840
BsaWI w'CCGG_w
Cuts at: 0 1140 1272
Size: 1140 132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 852 882 1272
Size: 852 30 390
Fragments arranged by size:
852 390 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 873 1272
Size: 873 399
BsiHKAI G_wGCw'C
Cuts at: 0 172 1272
Size: 172 1100
BsmI GAATG_Cn'
Cuts at: 0 824 940 1272
Size: 824 116 332
Fragments arranged by size:
824 332 116
Bsp1286I G_dGCh'C
Cuts at: 0 172 1272
Size: 172 1100
BspEI T'CCGG_A
Cuts at: 0 1140 1272
Size: 1140 132
BspHI T'CATG_A
Cuts at: 0 281 1272
Size: 281 991
BsrDI GCAATG_nn'
Cuts at: 0 77 1272
Size: 77 1195
BsrGI T'GTAC_A
Cuts at: 0 613 1272
Size: 613 659
BstEII G'GTnAC_C
Cuts at: 0 1011 1272
Size: 1011 261
BstYI r'GATC_y
Cuts at: 0 443 1166 1173 1272
Size: 443 723 7 99
Fragments arranged by size:
723 443 99 7
BstZ17I GTA'TAC
Cuts at: 0 53 1272
Size: 53 1219
BtgI C'CryG_G
Cuts at: 0 362 1272
Size: 362 910
EaeI y'GGCC_r
Cuts at: 0 900 1272
Size: 900 372
EarI CTCTTCn'nnn_
Cuts at: 0 410 421 1272
Size: 410 11 851
Fragments arranged by size:
851 410 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 659 781 838 991 1041 1272
Size: 445 214 122 57 153 50 231
Fragments arranged by size:
445 231 214 153 122 57 50
EcoO109I rG'GnC_Cy
Cuts at: 0 948 1061 1272
Size: 948 113 211
Fragments arranged by size:
948 211 113
EcoRI G'AATT_C
Cuts at: 0 268 1272
Size: 268 1004
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1158 1190 1272
Size: 1158 32 82
Fragments arranged by size:
1158 82 32
HincII GTy'rAC
Cuts at: 0 44 1272
Size: 44 1228
MfeI C'AATT_G
Cuts at: 0 609 1272
Size: 609 663
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 1272
Size: 175 1097
MscI TGG'CCA
Cuts at: 0 902 1272
Size: 902 370
MslI CAynn'nnrTG
Cuts at: 0 545 1033 1272
Size: 545 488 239
Fragments arranged by size:
545 488 239
NcoI C'CATG_G
Cuts at: 0 362 1272
Size: 362 910
NspI r_CATG'y
Cuts at: 0 990 1272
Size: 990 282
PciI A'CATG_T
Cuts at: 0 986 1272
Size: 986 286
PfoI T'CCnGG_A
Cuts at: 0 259 314 977 1272
Size: 259 55 663 295
Fragments arranged by size:
663 295 259 55
PsiI TTA'TAA
Cuts at: 0 220 394 1118 1272
Size: 220 174 724 154
Fragments arranged by size:
724 220 174 154
SapI GCTCTTCn'nnn_
Cuts at: 0 410 1272
Size: 410 862
SfcI C'TryA_G
Cuts at: 0 15 1272
Size: 15 1257
SmlI C'TyrA_G
Cuts at: 0 90 1133 1272
Size: 90 1043 139
Fragments arranged by size:
1043 139 90
SpeI A'CTAG_T
Cuts at: 0 105 1272
Size: 105 1167
StyI C'CwwG_G
Cuts at: 0 362 1153 1272
Size: 362 791 119
Fragments arranged by size:
791 362 119
TatI w'GTAC_w
Cuts at: 0 613 1272
Size: 613 659
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 852 884 1272
Size: 27 825 32 388
Fragments arranged by size:
825 388 32 27
XmnI GAAnn'nnTTC
Cuts at: 0 268 1272
Size: 268 1004
Enzymes that do cut:
AccI AcuI AflIII AleI AloI AlwNI ApoI AvrII
BamHI BanI BbsI BclI BglII BmgBI BpuEI BsaHI
BsaWI BsaXI BseRI BsiHKAI BsmI Bsp1286I BspEI BspHI
BsrDI BsrGI BstEII BstYI BstZ17I BtgI EaeI EarI
Eco57MI EcoO109I EcoRI Hin4I HincII MfeI MmeI MscI
MslI NcoI NspI PciI PfoI PsiI SapI SfcI
SmlI SpeI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI BaeI
BanII BbeI BbvCI BcgI BciVI BfrBI BglI BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI
BsaBI BseYI BsgI BsiEI BsiWI BsmBI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstXI Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MluI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI SspI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI ZraI