Restriction map for
pFN21AE6362
(Linear) (Six-base) MAPSORT of: KIEE6362.seq Check: 4582 from: 1 to: 1011
KIEE6362.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AccI GT'mk_AC
Cuts at: 0 638 1011
Size: 638 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 1011
Size: 812 199
AflIII A'CryG_T
Cuts at: 0 641 1011
Size: 641 370
ApaLI G'TGCA_C
Cuts at: 0 207 1011
Size: 207 804
ApoI r'AATT_y
Cuts at: 0 962 1011
Size: 962 49
AvaI C'yCGr_G
Cuts at: 0 192 1011
Size: 192 819
AvrII C'CTAG_G
Cuts at: 0 42 1011
Size: 42 969
BanI G'GyrC_C
Cuts at: 0 351 897 1011
Size: 351 546 114
Fragments arranged by size:
546 351 114
BanII G_rGCy'C
Cuts at: 0 80 1011
Size: 80 931
BbeI G_GCGC'C
Cuts at: 0 355 1011
Size: 355 656
BbsI GAAGACnn'nnnn_
Cuts at: 0 789 1011
Size: 789 222
BbvCI CC'TCA_GC
Cuts at: 0 777 1011
Size: 777 234
BciVI GTATCCnnnnn_n'
Cuts at: 0 245 1011
Size: 245 766
BglI GCCn_nnn'nGGC
Cuts at: 0 433 575 1011
Size: 433 142 436
Fragments arranged by size:
436 433 142
Bme1580I G_kGCm'C
Cuts at: 0 211 1011
Size: 211 800
BmrI ACTGGGnnnn_n'
Cuts at: 0 816 1011
Size: 816 195
BmtI G_CTAG'C
Cuts at: 0 751 1011
Size: 751 260
Bpu10I CC'TnA_GC
Cuts at: 0 777 1011
Size: 777 234
BsaHI Gr'CG_yC
Cuts at: 0 56 352 376 1011
Size: 56 296 24 635
Fragments arranged by size:
635 296 56 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 1011
Size: 31 980
BseYI C'CCAG_C
Cuts at: 0 721 1011
Size: 721 290
BsiHKAI G_wGCw'C
Cuts at: 0 211 1011
Size: 211 800
BsmBI CGTCTCn'nnnn_
Cuts at: 0 47 1011
Size: 47 964
Bsp1286I G_dGCh'C
Cuts at: 0 80 211 1011
Size: 80 131 800
Fragments arranged by size:
800 131 80
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 336 1011
Size: 336 675
BsrDI GCAATG_nn'
Cuts at: 0 702 890 1011
Size: 702 188 121
Fragments arranged by size:
702 188 121
BssSI C'ACGA_G
Cuts at: 0 613 1011
Size: 613 398
BstYI r'GATC_y
Cuts at: 0 150 1011
Size: 150 861
Bsu36I CC'TnA_GG
Cuts at: 0 410 1011
Size: 410 601
BtgI C'CryG_G
Cuts at: 0 356 1011
Size: 356 655
DraI TTT'AAA
Cuts at: 0 813 1011
Size: 813 198
EarI CTCTTCn'nnn_
Cuts at: 0 950 1011
Size: 950 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 256 1011
Size: 256 755
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 1011
Size: 812 199
EcoNI CCTnn'n_nnAGG
Cuts at: 0 10 1011
Size: 10 1001
EcoO109I rG'GnC_Cy
Cuts at: 0 773 818 1011
Size: 773 45 193
Fragments arranged by size:
773 193 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 488 1011
Size: 488 523
HaeII r_GCGC'y
Cuts at: 0 108 355 1011
Size: 108 247 656
Fragments arranged by size:
656 247 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 44 76 94 126 393 425 765 797
Size: 44 32 18 32 267 32 340 32
Cuts at: 797 1011
Size: 214
Fragments arranged by size:
340 267 214 44 32 32 32 32
18
HindIII A'AGCT_T
Cuts at: 0 260 316 1011
Size: 260 56 695
Fragments arranged by size:
695 260 56
KasI G'GCGC_C
Cuts at: 0 351 1011
Size: 351 660
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 997 1011
Size: 997 14
NarI GG'CG_CC
Cuts at: 0 352 1011
Size: 352 659
NheI G'CTAG_C
Cuts at: 0 747 1011
Size: 747 264
NspI r_CATG'y
Cuts at: 0 645 1011
Size: 645 366
PasI CC'CwG_GG
Cuts at: 0 841 1011
Size: 841 170
PciI A'CATG_T
Cuts at: 0 641 1011
Size: 641 370
PflMI CCAn_nnn'nTGG
Cuts at: 0 928 1011
Size: 928 83
PpuMI rG'GwC_Cy
Cuts at: 0 773 818 1011
Size: 773 45 193
Fragments arranged by size:
773 193 45
PspXI vC'TCGA_Gb
Cuts at: 0 192 1011
Size: 192 819
PstI C_TGCA'G
Cuts at: 0 333 1011
Size: 333 678
ScaI AGT'ACT
Cuts at: 0 943 1011
Size: 943 68
SfcI C'TryA_G
Cuts at: 0 329 945 1011
Size: 329 616 66
Fragments arranged by size:
616 329 66
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 575 1011
Size: 575 436
SfoI GGC'GCC
Cuts at: 0 353 1011
Size: 353 658
SmlI C'TyrA_G
Cuts at: 0 192 1011
Size: 192 819
StyI C'CwwG_G
Cuts at: 0 42 580 1011
Size: 42 538 431
Fragments arranged by size:
538 431 42
TatI w'GTAC_w
Cuts at: 0 871 941 1011
Size: 871 70 70
Fragments arranged by size:
871 70 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 881 913 1011
Size: 881 32 98
Fragments arranged by size:
881 98 32
Tth111I GACn'n_nGTC
Cuts at: 0 58 1011
Size: 58 953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 562 848 1011
Size: 562 286 163
Fragments arranged by size:
562 286 163
XhoI C'TCGA_G
Cuts at: 0 192 1011
Size: 192 819
Enzymes that do cut:
AccI AcuI AflIII ApaLI ApoI AvaI AvrII BanI
BanII BbeI BbsI BbvCI BciVI BglI Bme1580I BmrI
BmtI Bpu10I BsaHI BseRI BseYI BsiHKAI BsmBI Bsp1286I
BspMI BsrDI BssSI BstYI Bsu36I BtgI DraI EarI
EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII
KasI MmeI NarI NheI NspI PasI PciI PflMI
PpuMI PspXI PstI ScaI SfcI SfiI SfoI SmlI
StyI TatI TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
BaeI BamHI BcgI BclI BfrBI BglII BlpI BmgBI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EcoICRI
EcoRI EcoRV FseI FspI FspAI HincII HpaI KpnI
MfeI MluI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NotI NruI NsiI PacI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII XbaI XmaI XmnI ZraI