Restriction map for
pFN21AE6361
(Linear) (Six-base) MAPSORT of: KIEE6361.seq Check: 3287 from: 1 to: 1044
KIEE6361.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 751 1044
Size: 751 293
AflII C'TTAA_G
Cuts at: 0 205 1044
Size: 205 839
AseI AT'TA_AT
Cuts at: 0 814 1044
Size: 814 230
AvaI C'yCGr_G
Cuts at: 0 698 1044
Size: 698 346
BanI G'GyrC_C
Cuts at: 0 241 1044
Size: 241 803
BanII G_rGCy'C
Cuts at: 0 14 46 1044
Size: 14 32 998
Fragments arranged by size:
998 32 14
BbvCI CC'TCA_GC
Cuts at: 0 131 1044
Size: 131 913
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 208 242 1044
Size: 208 34 802
Fragments arranged by size:
802 208 34
Bme1580I G_kGCm'C
Cuts at: 0 244 1044
Size: 244 800
BmtI G_CTAG'C
Cuts at: 0 383 1044
Size: 383 661
Bpu10I CC'TnA_GC
Cuts at: 0 16 131 1044
Size: 16 115 913
Fragments arranged by size:
913 115 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 950 1044
Size: 950 94
BsaI GGTCTCn'nnnn_
Cuts at: 0 230 1044
Size: 230 814
BsaAI yAC'GTr
Cuts at: 0 994 1044
Size: 994 50
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 735 1044
Size: 735 309
BseYI C'CCAG_C
Cuts at: 0 6 122 629 1044
Size: 6 116 507 415
Fragments arranged by size:
507 415 116 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 779 1044
Size: 351 428 265
Fragments arranged by size:
428 351 265
BsiHKAI G_wGCw'C
Cuts at: 0 46 1044
Size: 46 998
Bsp1286I G_dGCh'C
Cuts at: 0 14 46 244 1044
Size: 14 32 198 800
Fragments arranged by size:
800 198 32 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 751 1044
Size: 751 293
BsrFI r'CCGG_y
Cuts at: 0 108 1044
Size: 108 936
BstYI r'GATC_y
Cuts at: 0 1001 1044
Size: 1001 43
BtgI C'CryG_G
Cuts at: 0 268 1044
Size: 268 776
BtsI GCAGTG_nn'
Cuts at: 0 363 545 1044
Size: 363 182 499
Fragments arranged by size:
499 363 182
DraIII CAC_nnn'GTG
Cuts at: 0 614 1044
Size: 614 430
EaeI y'GGCC_r
Cuts at: 0 265 297 996 1044
Size: 265 32 699 48
Fragments arranged by size:
699 265 48 32
EarI CTCTTCn'nnn_
Cuts at: 0 882 1044
Size: 882 162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 174 1044
Size: 174 870
EcoICRI GAG'CTC
Cuts at: 0 44 1044
Size: 44 1000
EcoNI CCTnn'n_nnAGG
Cuts at: 0 78 1044
Size: 78 966
EcoO109I rG'GnC_Cy
Cuts at: 0 348 1044
Size: 348 696
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 991 1044
Size: 991 53
HaeII r_GCGC'y
Cuts at: 0 28 1044
Size: 28 1016
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 1044
Size: 52 32 960
Fragments arranged by size:
960 52 32
MscI TGG'CCA
Cuts at: 0 998 1044
Size: 998 46
MslI CAynn'nnrTG
Cuts at: 0 267 317 816 878 1044
Size: 267 50 499 62 166
Fragments arranged by size:
499 267 166 62 50
MspA1I CmG'CkG
Cuts at: 0 358 403 1044
Size: 358 45 641
Fragments arranged by size:
641 358 45
NheI G'CTAG_C
Cuts at: 0 379 1044
Size: 379 665
NspI r_CATG'y
Cuts at: 0 181 1044
Size: 181 863
PflMI CCAn_nnn'nTGG
Cuts at: 0 597 1044
Size: 597 447
PfoI T'CCnGG_A
Cuts at: 0 596 1044
Size: 596 448
PpuMI rG'GwC_Cy
Cuts at: 0 348 1044
Size: 348 696
PsiI TTA'TAA
Cuts at: 0 622 1044
Size: 622 422
PstI C_TGCA'G
Cuts at: 0 287 1044
Size: 287 757
PvuII CAG'CTG
Cuts at: 0 358 403 1044
Size: 358 45 641
Fragments arranged by size:
641 358 45
SacI G_AGCT'C
Cuts at: 0 46 1044
Size: 46 998
SfcI C'TryA_G
Cuts at: 0 283 1044
Size: 283 761
SmlI C'TyrA_G
Cuts at: 0 205 698 965 1044
Size: 205 493 267 79
Fragments arranged by size:
493 267 205 79
SphI G_CATG'C
Cuts at: 0 181 1044
Size: 181 863
SspI AAT'ATT
Cuts at: 0 666 987 1044
Size: 666 321 57
Fragments arranged by size:
666 321 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 261 1044
Size: 261 783
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 317 349 1044
Size: 317 32 695
Fragments arranged by size:
695 317 32
XhoI C'TCGA_G
Cuts at: 0 698 1044
Size: 698 346
Enzymes that do cut:
AarI AflII AseI AvaI BanI BanII BbvCI BcgI
Bme1580I BmtI Bpu10I BpuEI BsaI BsaAI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrFI BstYI BtgI BtsI
DraIII EaeI EarI EciI EcoICRI EcoNI EcoO109I FalI
HaeII Hin4I MscI MslI MspA1I NheI NspI PflMI
PfoI PpuMI PsiI PstI PvuII SacI SfcI SmlI
SphI SspI TaqII TstI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AsiSI AvrII BaeI BamHI BbeI BbsI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BplI
BpmI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI Eco57MI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI StyI SwaI
TatI Tth111I XbaI XcmI XmaI XmnI ZraI