Restriction map for
pFN21AE6360
(Linear) (Six-base) MAPSORT of: KIEE6360.seq Check: 5352 from: 1 to: 972
KIEE6360.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 640 972
Size: 301 339 332
Fragments arranged by size:
339 332 301
AfeI AGC'GCT
Cuts at: 0 434 972
Size: 434 538
AleI CACnn'nnGTG
Cuts at: 0 379 972
Size: 379 593
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 972
Size: 397 575
AlwNI CAG_nnn'CTG
Cuts at: 0 351 523 526 642 972
Size: 351 172 3 116 330
Fragments arranged by size:
351 330 172 116 3
ApaI G_GGCC'C
Cuts at: 0 239 972
Size: 239 733
AvaI C'yCGr_G
Cuts at: 0 490 972
Size: 490 482
BanI G'GyrC_C
Cuts at: 0 91 248 404 972
Size: 91 157 156 568
Fragments arranged by size:
568 157 156 91
BanII G_rGCy'C
Cuts at: 0 239 972
Size: 239 733
BbeI G_GCGC'C
Cuts at: 0 252 972
Size: 252 720
BbvCI CC'TCA_GC
Cuts at: 0 153 972
Size: 153 819
BglI GCCn_nnn'nGGC
Cuts at: 0 144 234 972
Size: 144 90 738
Fragments arranged by size:
738 144 90
BlpI GC'TnA_GC
Cuts at: 0 772 972
Size: 772 200
Bme1580I G_kGCm'C
Cuts at: 0 96 140 239 972
Size: 96 44 99 733
Fragments arranged by size:
733 99 96 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 500 972
Size: 500 472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 972
Size: 500 472
Bpu10I CC'TnA_GC
Cuts at: 0 153 972
Size: 153 819
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 972
Size: 346 626
BsaHI Gr'CG_yC
Cuts at: 0 249 972
Size: 249 723
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 141 562 972
Size: 141 421 410
Fragments arranged by size:
421 410 141
BseYI C'CCAG_C
Cuts at: 0 334 972
Size: 334 638
BsiEI CG_ry'CG
Cuts at: 0 132 972
Size: 132 840
BsiHKAI G_wGCw'C
Cuts at: 0 386 878 972
Size: 386 492 94
Fragments arranged by size:
492 386 94
Bsp1286I G_dGCh'C
Cuts at: 0 96 140 239 386 878 972
Size: 96 44 99 147 492 94
Fragments arranged by size:
492 147 99 96 94 44
BspHI T'CATG_A
Cuts at: 0 626 809 972
Size: 626 183 163
Fragments arranged by size:
626 183 163
BsrDI GCAATG_nn'
Cuts at: 0 103 928 972
Size: 103 825 44
Fragments arranged by size:
825 103 44
BsrGI T'GTAC_A
Cuts at: 0 611 972
Size: 611 361
BssSI C'ACGA_G
Cuts at: 0 763 972
Size: 763 209
BstAPI GCAn_nnn'nTGC
Cuts at: 0 526 972
Size: 526 446
BtsI GCAGTG_nn'
Cuts at: 0 77 972
Size: 77 895
EaeI y'GGCC_r
Cuts at: 0 129 923 944 972
Size: 129 794 21 28
Fragments arranged by size:
794 129 28 21
EagI C'GGCC_G
Cuts at: 0 129 972
Size: 129 843
EarI CTCTTCn'nnn_
Cuts at: 0 670 972
Size: 670 302
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 500 640 972
Size: 301 199 140 332
Fragments arranged by size:
332 301 199 140
EcoO109I rG'GnC_Cy
Cuts at: 0 235 236 477 972
Size: 235 1 241 495
Fragments arranged by size:
495 241 235 1
HaeII r_GCGC'y
Cuts at: 0 252 293 436 972
Size: 252 41 143 536
Fragments arranged by size:
536 252 143 41
HincII GTy'rAC
Cuts at: 0 867 972
Size: 867 105
KasI G'GCGC_C
Cuts at: 0 248 972
Size: 248 724
MscI TGG'CCA
Cuts at: 0 925 946 972
Size: 925 21 26
Fragments arranged by size:
925 26 21
MslI CAynn'nnrTG
Cuts at: 0 82 379 972
Size: 82 297 593
Fragments arranged by size:
593 297 82
MspA1I CmG'CkG
Cuts at: 0 53 379 523 639 972
Size: 53 326 144 116 333
Fragments arranged by size:
333 326 144 116 53
NarI GG'CG_CC
Cuts at: 0 249 972
Size: 249 723
NspI r_CATG'y
Cuts at: 0 709 972
Size: 709 263
PasI CC'CwG_GG
Cuts at: 0 231 972
Size: 231 741
PfoI T'CCnGG_A
Cuts at: 0 734 972
Size: 734 238
PspOMI G'GGCC_C
Cuts at: 0 235 972
Size: 235 737
PstI C_TGCA'G
Cuts at: 0 902 972
Size: 902 70
PvuII CAG'CTG
Cuts at: 0 379 523 972
Size: 379 144 449
Fragments arranged by size:
449 379 144
SexAI A'CCwGG_T
Cuts at: 0 302 344 972
Size: 302 42 628
Fragments arranged by size:
628 302 42
SfcI C'TryA_G
Cuts at: 0 898 972
Size: 898 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 234 972
Size: 234 738
SfoI GGC'GCC
Cuts at: 0 250 972
Size: 250 722
SmaI CCC'GGG
Cuts at: 0 492 972
Size: 492 480
SmlI C'TyrA_G
Cuts at: 0 361 972
Size: 361 611
SphI G_CATG'C
Cuts at: 0 709 972
Size: 709 263
StuI AGG'CCT
Cuts at: 0 29 563 731 972
Size: 29 534 168 241
Fragments arranged by size:
534 241 168 29
TatI w'GTAC_w
Cuts at: 0 581 611 956 972
Size: 581 30 345 16
Fragments arranged by size:
581 345 30 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 784 972
Size: 784 188
XmaI C'CCGG_G
Cuts at: 0 490 972
Size: 490 482
XmnI GAAnn'nnTTC
Cuts at: 0 664 972
Size: 664 308
Enzymes that do cut:
AcuI AfeI AleI AlfI AlwNI ApaI AvaI BanI
BanII BbeI BbvCI BglI BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaHI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BsrDI BsrGI BssSI BstAPI BtsI EaeI EagI
EarI Eco57MI EcoO109I HaeII HincII KasI MscI MslI
MspA1I NarI NspI PasI PfoI PspOMI PstI PvuII
SexAI SfcI SfiI SfoI SmaI SmlI SphI StuI
TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI BsaXI
BsgI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI
BssHII BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SgfI SgrAI SnaBI SpeI SrfI SspI
StyI SwaI TaqII TstI Tth111I XbaI XhoI ZraI