Restriction map for
pFN21AE6357
(Linear) (Six-base) MAPSORT of: KIEE6357.seq Check: 35 from: 1 to: 975
KIEE6357.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 530 603 801 975
Size: 530 73 198 174
Fragments arranged by size:
530 198 174 73
AccI GT'mk_AC
Cuts at: 0 956 975
Size: 956 19
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 975
Size: 799 176
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 562 975
Size: 562 413
BanI G'GyrC_C
Cuts at: 0 297 801 975
Size: 297 504 174
Fragments arranged by size:
504 297 174
BanII G_rGCy'C
Cuts at: 0 84 883 921 975
Size: 84 799 38 54
Fragments arranged by size:
799 84 54 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 352 975
Size: 352 623
BclI T'GATC_A
Cuts at: 0 746 975
Size: 746 229
Bme1580I G_kGCm'C
Cuts at: 0 135 302 551 804 864 975
Size: 135 167 249 253 60 111
Fragments arranged by size:
253 249 167 135 111 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 781 975
Size: 497 284 194
Fragments arranged by size:
497 284 194
BsaWI w'CCGG_w
Cuts at: 0 249 975
Size: 249 726
BseYI C'CCAG_C
Cuts at: 0 53 447 795 940 975
Size: 53 394 348 145 35
Fragments arranged by size:
394 348 145 53 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 975
Size: 181 794
BsiHKAI G_wGCw'C
Cuts at: 0 883 921 975
Size: 883 38 54
Fragments arranged by size:
883 54 38
Bsp1286I G_dGCh'C
Cuts at: 0 84 135 302 551 804 864 883 921
Size: 84 51 167 249 253 60 19 38
Cuts at: 921 975
Size: 54
Fragments arranged by size:
253 249 167 84 60 54 51 38
19
BspEI T'CCGG_A
Cuts at: 0 249 975
Size: 249 726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 530 603 631 801 975
Size: 530 73 28 170 174
Fragments arranged by size:
530 174 170 73 28
BsrFI r'CCGG_y
Cuts at: 0 175 975
Size: 175 800
BstEII G'GTnAC_C
Cuts at: 0 183 975
Size: 183 792
BstXI CCAn_nnnn'nTGG
Cuts at: 0 495 975
Size: 495 480
BstYI r'GATC_y
Cuts at: 0 923 975
Size: 923 52
BtgI C'CryG_G
Cuts at: 0 356 488 728 975
Size: 356 132 240 247
Fragments arranged by size:
356 247 240 132
BtsI GCAGTG_nn'
Cuts at: 0 376 423 566 975
Size: 376 47 143 409
Fragments arranged by size:
409 376 143 47
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 399 434 975
Size: 399 35 541
Fragments arranged by size:
541 399 35
EaeI y'GGCC_r
Cuts at: 0 279 485 731 975
Size: 279 206 246 244
Fragments arranged by size:
279 246 244 206
EarI CTCTTCn'nnn_
Cuts at: 0 871 975
Size: 871 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 228 975
Size: 228 747
EcoICRI GAG'CTC
Cuts at: 0 881 919 975
Size: 881 38 56
Fragments arranged by size:
881 56 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 781 799 975
Size: 497 284 18 176
Fragments arranged by size:
497 284 176 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 789 975
Size: 789 186
HaeII r_GCGC'y
Cuts at: 0 786 975
Size: 786 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 908 940 975
Size: 908 32 35
Fragments arranged by size:
908 35 32
HincII GTy'rAC
Cuts at: 0 402 975
Size: 402 573
HindIII A'AGCT_T
Cuts at: 0 143 975
Size: 143 832
HpaI GTT'AAC
Cuts at: 0 402 975
Size: 402 573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 975
Size: 434 541
MscI TGG'CCA
Cuts at: 0 487 733 975
Size: 487 246 242
Fragments arranged by size:
487 246 242
MslI CAynn'nnrTG
Cuts at: 0 307 436 625 975
Size: 307 129 189 350
Fragments arranged by size:
350 307 189 129
MspA1I CmG'CkG
Cuts at: 0 138 210 447 568 975
Size: 138 72 237 121 407
Fragments arranged by size:
407 237 138 121 72
NaeI GCC'GGC
Cuts at: 0 177 975
Size: 177 798
NcoI C'CATG_G
Cuts at: 0 728 975
Size: 728 247
NgoMIV G'CCGG_C
Cuts at: 0 175 975
Size: 175 800
NspI r_CATG'y
Cuts at: 0 288 330 975
Size: 288 42 645
Fragments arranged by size:
645 288 42
PasI CC'CwG_GG
Cuts at: 0 190 495 975
Size: 190 305 480
Fragments arranged by size:
480 305 190
PfoI T'CCnGG_A
Cuts at: 0 41 975
Size: 41 934
PstI C_TGCA'G
Cuts at: 0 614 672 812 975
Size: 614 58 140 163
Fragments arranged by size:
614 163 140 58
PvuII CAG'CTG
Cuts at: 0 138 210 447 568 975
Size: 138 72 237 121 407
Fragments arranged by size:
407 237 138 121 72
SacI G_AGCT'C
Cuts at: 0 883 921 975
Size: 883 38 54
Fragments arranged by size:
883 54 38
SapI GCTCTTCn'nnn_
Cuts at: 0 871 975
Size: 871 104
SbfI CC_TGCA'GG
Cuts at: 0 812 975
Size: 812 163
ScaI AGT'ACT
Cuts at: 0 66 975
Size: 66 909
SfcI C'TryA_G
Cuts at: 0 168 339 610 668 808 975
Size: 168 171 271 58 140 167
Fragments arranged by size:
271 171 168 167 140 58
SphI G_CATG'C
Cuts at: 0 330 975
Size: 330 645
StyI C'CwwG_G
Cuts at: 0 530 728 975
Size: 530 198 247
Fragments arranged by size:
530 247 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4 363 975
Size: 4 359 612
Fragments arranged by size:
612 359 4
TatI w'GTAC_w
Cuts at: 0 64 975
Size: 64 911
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 196 228 975
Size: 196 32 747
Fragments arranged by size:
747 196 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 480 948 975
Size: 480 468 27
Fragments arranged by size:
480 468 27
XmnI GAAnn'nnTTC
Cuts at: 0 772 975
Size: 772 203
Enzymes that do cut:
AarI AccI AcuI AlfI BanI BanII BbsI BclI
Bme1580I BpmI BsaWI BseYI BsgI BsiHKAI Bsp1286I BspEI
BspMI BsrFI BstEII BstXI BstYI BtgI BtsI CspCI
EaeI EarI EciI EcoICRI Eco57MI FalI HaeII Hin4I
HincII HindIII HpaI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PfoI PstI PvuII SacI
SapI SbfI ScaI SfcI SphI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI
ClaI DraI DraIII DrdI EagI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI
ZraI