Restriction map for
pFN21AE6356
(Linear) (Six-base) MAPSORT of: KIEE6356.seq Check: 1066 from: 1 to: 963
KIEE6356.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AccI GT'mk_AC
Cuts at: 0 761 963
Size: 761 202
Acc65I G'GTAC_C
Cuts at: 0 833 963
Size: 833 130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 963
Size: 595 368
AflIII A'CryG_T
Cuts at: 0 182 963
Size: 182 781
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 947 963
Size: 947 16
AlwNI CAG_nnn'CTG
Cuts at: 0 829 963
Size: 829 134
ApaI G_GGCC'C
Cuts at: 0 640 963
Size: 640 323
ApaLI G'TGCA_C
Cuts at: 0 178 271 963
Size: 178 93 692
Fragments arranged by size:
692 178 93
AvaI C'yCGr_G
Cuts at: 0 595 632 640 963
Size: 595 37 8 323
Fragments arranged by size:
595 323 37 8
BamHI G'GATC_C
Cuts at: 0 419 467 791 963
Size: 419 48 324 172
Fragments arranged by size:
419 324 172 48
BanI G'GyrC_C
Cuts at: 0 694 833 963
Size: 694 139 130
Fragments arranged by size:
694 139 130
BanII G_rGCy'C
Cuts at: 0 392 640 823 963
Size: 392 248 183 140
Fragments arranged by size:
392 248 183 140
BglI GCCn_nnn'nGGC
Cuts at: 0 10 599 963
Size: 10 589 364
Fragments arranged by size:
589 364 10
BlpI GC'TnA_GC
Cuts at: 0 202 963
Size: 202 761
Bme1580I G_kGCm'C
Cuts at: 0 44 182 275 633 640 963
Size: 44 138 93 358 7 323
Fragments arranged by size:
358 323 138 93 44 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 204 963
Size: 204 759
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 324 396 963
Size: 307 17 72 567
Fragments arranged by size:
567 307 72 17
BsaI GGTCTCn'nnnn_
Cuts at: 0 562 963
Size: 562 401
BsaAI yAC'GTr
Cuts at: 0 183 783 963
Size: 183 600 180
Fragments arranged by size:
600 183 180
BsaWI w'CCGG_w
Cuts at: 0 470 963
Size: 470 493
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 223 253 285 315 963
Size: 223 30 32 30 648
Fragments arranged by size:
648 223 32 30 30
BseYI C'CCAG_C
Cuts at: 0 476 488 722 963
Size: 476 12 234 241
Fragments arranged by size:
476 241 234 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 724 907 963
Size: 319 405 183 56
Fragments arranged by size:
405 319 183 56
BsiEI CG_ry'CG
Cuts at: 0 85 108 137 741 963
Size: 85 23 29 604 222
Fragments arranged by size:
604 222 85 29 23
BsiHKAI G_wGCw'C
Cuts at: 0 182 221 275 392 963
Size: 182 39 54 117 571
Fragments arranged by size:
571 182 117 54 39
Bsp1286I G_dGCh'C
Cuts at: 0 44 182 221 275 392 633 640 823
Size: 44 138 39 54 117 241 7 183
Cuts at: 823 963
Size: 140
Fragments arranged by size:
241 183 140 138 117 54 44 39
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 78 580 963
Size: 78 502 383
Fragments arranged by size:
502 383 78
BsrFI r'CCGG_y
Cuts at: 0 80 136 484 963
Size: 80 56 348 479
Fragments arranged by size:
479 348 80 56
BssSI C'ACGA_G
Cuts at: 0 767 963
Size: 767 196
BstEII G'GTnAC_C
Cuts at: 0 480 963
Size: 480 483
BstYI r'GATC_y
Cuts at: 0 419 467 791 963
Size: 419 48 324 172
Fragments arranged by size:
419 324 172 48
Bsu36I CC'TnA_GG
Cuts at: 0 55 348 963
Size: 55 293 615
Fragments arranged by size:
615 293 55
BtgI C'CryG_G
Cuts at: 0 148 554 963
Size: 148 406 409
Fragments arranged by size:
409 406 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 360 963
Size: 360 603
BtsI GCAGTG_nn'
Cuts at: 0 196 231 963
Size: 196 35 732
Fragments arranged by size:
732 196 35
EaeI y'GGCC_r
Cuts at: 0 78 82 105 134 145 738 908 963
Size: 78 4 23 29 11 593 170 55
Fragments arranged by size:
593 170 78 55 29 23 11 4
EagI C'GGCC_G
Cuts at: 0 82 105 134 738 963
Size: 82 23 29 604 225
Fragments arranged by size:
604 225 82 29 23
EarI CTCTTCn'nnn_
Cuts at: 0 883 963
Size: 883 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 154 784 963
Size: 154 630 179
Fragments arranged by size:
630 179 154
EcoICRI GAG'CTC
Cuts at: 0 390 963
Size: 390 573
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 324 396 595 963
Size: 307 17 72 199 368
Fragments arranged by size:
368 307 199 72 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 439 538 963
Size: 439 99 425
Fragments arranged by size:
439 425 99
EcoO109I rG'GnC_Cy
Cuts at: 0 11 245 636 637 670 963
Size: 11 234 391 1 33 293
Fragments arranged by size:
391 293 234 33 11 1
FseI GG_CCGG'CC
Cuts at: 0 84 963
Size: 84 879
KpnI G_GTAC'C
Cuts at: 0 837 963
Size: 837 126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 963
Size: 284 679
MscI TGG'CCA
Cuts at: 0 147 910 963
Size: 147 763 53
Fragments arranged by size:
763 147 53
MslI CAynn'nnrTG
Cuts at: 0 147 963
Size: 147 816
MspA1I CmG'CkG
Cuts at: 0 226 368 963
Size: 226 142 595
Fragments arranged by size:
595 226 142
NaeI GCC'GGC
Cuts at: 0 82 138 963
Size: 82 56 825
Fragments arranged by size:
825 82 56
NcoI C'CATG_G
Cuts at: 0 148 963
Size: 148 815
NgoMIV G'CCGG_C
Cuts at: 0 80 136 963
Size: 80 56 827
Fragments arranged by size:
827 80 56
NotI GC'GGCC_GC
Cuts at: 0 738 963
Size: 738 225
NspI r_CATG'y
Cuts at: 0 624 963
Size: 624 339
PasI CC'CwG_GG
Cuts at: 0 463 963
Size: 463 500
PflMI CCAn_nnn'nTGG
Cuts at: 0 76 963
Size: 76 887
PfoI T'CCnGG_A
Cuts at: 0 437 963
Size: 437 526
PmlI CAC'GTG
Cuts at: 0 183 963
Size: 183 780
PpuMI rG'GwC_Cy
Cuts at: 0 245 963
Size: 245 718
PspOMI G'GGCC_C
Cuts at: 0 636 963
Size: 636 327
PvuII CAG'CTG
Cuts at: 0 226 963
Size: 226 737
SacI G_AGCT'C
Cuts at: 0 392 963
Size: 392 571
SanDI GG'GwC_CC
Cuts at: 0 245 963
Size: 245 718
SexAI A'CCwGG_T
Cuts at: 0 266 963
Size: 266 697
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 10 963
Size: 10 953
SmaI CCC'GGG
Cuts at: 0 597 634 642 963
Size: 597 37 8 321
Fragments arranged by size:
597 321 37 8
StuI AGG'CCT
Cuts at: 0 445 963
Size: 445 518
StyI C'CwwG_G
Cuts at: 0 6 148 963
Size: 6 142 815
Fragments arranged by size:
815 142 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 204 468 690 941 963
Size: 204 264 222 251 22
Fragments arranged by size:
264 251 222 204 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 223 255 285 317 963
Size: 223 32 30 32 646
Fragments arranged by size:
646 223 32 32 30
XmaI C'CCGG_G
Cuts at: 0 595 632 640 963
Size: 595 37 8 323
Fragments arranged by size:
595 323 37 8
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlfI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BglI BlpI Bme1580I BmrI
BpmI BsaI BsaAI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrFI BssSI BstEII BstYI Bsu36I
BtgI BtgZI BtsI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FseI KpnI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NotI NspI PasI PflMI
PfoI PmlI PpuMI PspOMI PvuII SacI SanDI SexAI
SfiI SmaI StuI StyI TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AloI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI
BmtI BplI Bpu10I BpuEI BsaBI BsaHI BseRI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BstAPI BstBI BstXI BstZ17I ClaI CspCI DraI DraIII
DrdI EcoRI EcoRV FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI MfeI MluI NarI NdeI
NheI NruI NsiI PacI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XcmI XhoI XmnI ZraI