Restriction map for
pFN21AE6352
(Linear) (Six-base) MAPSORT of: KIEE6352.seq Check: 3774 from: 1 to: 1110
KIEE6352.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 6 1110
Size: 6 1104
AflIII A'CryG_T
Cuts at: 0 443 527 1110
Size: 443 84 583
Fragments arranged by size:
583 443 84
AleI CACnn'nnGTG
Cuts at: 0 625 1110
Size: 625 485
AlwNI CAG_nnn'CTG
Cuts at: 0 1068 1110
Size: 1068 42
ApoI r'AATT_y
Cuts at: 0 398 1087 1110
Size: 398 689 23
Fragments arranged by size:
689 398 23
BanI G'GyrC_C
Cuts at: 0 589 787 1110
Size: 589 198 323
Fragments arranged by size:
589 323 198
BanII G_rGCy'C
Cuts at: 0 884 1110
Size: 884 226
BbsI GAAGACnn'nnnn_
Cuts at: 0 1061 1110
Size: 1061 49
BfrBI ATG'CAT
Cuts at: 0 1101 1110
Size: 1101 9
BlpI GC'TnA_GC
Cuts at: 0 808 1110
Size: 808 302
Bme1580I G_kGCm'C
Cuts at: 0 594 673 1110
Size: 594 79 437
Fragments arranged by size:
594 437 79
BmtI G_CTAG'C
Cuts at: 0 193 1110
Size: 193 917
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 690 1110
Size: 690 420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 1110
Size: 784 326
Bpu10I CC'TnA_GC
Cuts at: 0 326 1110
Size: 326 784
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 839 1110
Size: 839 271
BsaAI yAC'GTr
Cuts at: 0 444 1110
Size: 444 666
BsaHI Gr'CG_yC
Cuts at: 0 5 1110
Size: 5 1105
BseYI C'CCAG_C
Cuts at: 0 613 648 1110
Size: 613 35 462
Fragments arranged by size:
613 462 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 1110
Size: 715 395
BsmBI CGTCTCn'nnnn_
Cuts at: 0 457 1110
Size: 457 653
Bsp1286I G_dGCh'C
Cuts at: 0 594 673 884 1110
Size: 594 79 211 226
Fragments arranged by size:
594 226 211 79
BspHI T'CATG_A
Cuts at: 0 1079 1110
Size: 1079 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 6 653 1110
Size: 6 647 457
Fragments arranged by size:
647 457 6
BsrDI GCAATG_nn'
Cuts at: 0 930 1110
Size: 930 180
BsrGI T'GTAC_A
Cuts at: 0 716 1110
Size: 716 394
BstEII G'GTnAC_C
Cuts at: 0 618 1110
Size: 618 492
BstXI CCAn_nnnn'nTGG
Cuts at: 0 18 416 555 1110
Size: 18 398 139 555
Fragments arranged by size:
555 398 139 18
BstYI r'GATC_y
Cuts at: 0 405 1110
Size: 405 705
BtgI C'CryG_G
Cuts at: 0 623 791 797 1110
Size: 623 168 6 313
Fragments arranged by size:
623 313 168 6
DraIII CAC_nnn'GTG
Cuts at: 0 60 1110
Size: 60 1050
EaeI y'GGCC_r
Cuts at: 0 240 794 1070 1110
Size: 240 554 276 40
Fragments arranged by size:
554 276 240 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 1110
Size: 784 326
FspI TGC'GCA
Cuts at: 0 456 1110
Size: 456 654
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 517 549 1110
Size: 517 32 561
Fragments arranged by size:
561 517 32
HincII GTy'rAC
Cuts at: 0 24 1110
Size: 24 1086
HindIII A'AGCT_T
Cuts at: 0 805 1110
Size: 805 305
MfeI C'AATT_G
Cuts at: 0 1049 1110
Size: 1049 61
MslI CAynn'nnrTG
Cuts at: 0 625 1110
Size: 625 485
MspA1I CmG'CkG
Cuts at: 0 793 1110
Size: 793 317
NcoI C'CATG_G
Cuts at: 0 797 1110
Size: 797 313
NheI G'CTAG_C
Cuts at: 0 189 1110
Size: 189 921
NsiI A_TGCA'T
Cuts at: 0 1103 1110
Size: 1103 7
NspI r_CATG'y
Cuts at: 0 531 1110
Size: 531 579
PciI A'CATG_T
Cuts at: 0 527 1110
Size: 527 583
PfoI T'CCnGG_A
Cuts at: 0 680 1110
Size: 680 430
SacII CC_GC'GG
Cuts at: 0 794 1110
Size: 794 316
ScaI AGT'ACT
Cuts at: 0 515 1110
Size: 515 595
SmlI C'TyrA_G
Cuts at: 0 854 1110
Size: 854 256
SspI AAT'ATT
Cuts at: 0 906 1110
Size: 906 204
StyI C'CwwG_G
Cuts at: 0 797 1110
Size: 797 313
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 224 666 1110
Size: 224 442 444
Fragments arranged by size:
444 442 224
TatI w'GTAC_w
Cuts at: 0 513 716 1110
Size: 513 203 394
Fragments arranged by size:
513 394 203
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 601 1110
Size: 601 509
XmnI GAAnn'nnTTC
Cuts at: 0 1058 1110
Size: 1058 52
Enzymes that do cut:
AarI AflIII AleI AlwNI ApoI BanI BanII BbsI
BfrBI BlpI Bme1580I BmtI BplI BpmI Bpu10I BpuEI
BsaAI BsaHI BseYI BsgI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrGI BstEII BstXI BstYI BtgI DraIII EaeI
Eco57MI FspI Hin4I HincII HindIII MfeI MslI MspA1I
NcoI NheI NsiI NspI PciI PfoI SacII ScaI
SmlI SspI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BglI BglII BmgBI BmrI BsaI BsaBI BsaWI
BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI BspEI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DrdI EagI EarI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI
HaeII HpaI KasI KpnI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NotI NruI PacI PasI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI
Tth111I XbaI XhoI XmaI ZraI