Restriction map for
pFN21AE6346
(Linear) (Six-base) MAPSORT of: KIEE6346.seq Check: 2796 from: 1 to: 1296
KIEE6346.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 46 1296
Size: 46 1250
AccI GT'mk_AC
Cuts at: 0 1226 1296
Size: 1226 70
Acc65I G'GTAC_C
Cuts at: 0 150 1296
Size: 150 1146
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 1296
Size: 162 1134
AfeI AGC'GCT
Cuts at: 0 442 1296
Size: 442 854
AflII C'TTAA_G
Cuts at: 0 610 1296
Size: 610 686
AgeI A'CCGG_T
Cuts at: 0 202 551 1296
Size: 202 349 745
Fragments arranged by size:
745 349 202
AleI CACnn'nnGTG
Cuts at: 0 634 1174 1296
Size: 634 540 122
Fragments arranged by size:
634 540 122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 775 1296
Size: 775 521
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 442 474 1296
Size: 442 32 822
Fragments arranged by size:
822 442 32
AlwNI CAG_nnn'CTG
Cuts at: 0 327 462 639 1296
Size: 327 135 177 657
Fragments arranged by size:
657 327 177 135
ApaI G_GGCC'C
Cuts at: 0 969 1296
Size: 969 327
AvrII C'CTAG_G
Cuts at: 0 48 1296
Size: 48 1248
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 532 565 1296
Size: 532 33 731
Fragments arranged by size:
731 532 33
BanI G'GyrC_C
Cuts at: 0 150 282 540 593 1296
Size: 150 132 258 53 703
Fragments arranged by size:
703 258 150 132 53
BanII G_rGCy'C
Cuts at: 0 257 356 782 949 969 1296
Size: 257 99 426 167 20 327
Fragments arranged by size:
426 327 257 167 99 20
BbeI G_GCGC'C
Cuts at: 0 286 1296
Size: 286 1010
BbsI GAAGACnn'nnnn_
Cuts at: 0 1089 1296
Size: 1089 207
BlpI GC'TnA_GC
Cuts at: 0 583 1296
Size: 583 713
Bme1580I G_kGCm'C
Cuts at: 0 543 969 1296
Size: 543 426 327
Fragments arranged by size:
543 426 327
BmrI ACTGGGnnnn_n'
Cuts at: 0 720 1296
Size: 720 576
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 340 994 1296
Size: 217 123 654 302
Fragments arranged by size:
654 302 217 123
BsaI GGTCTCn'nnnn_
Cuts at: 0 193 298 1296
Size: 193 105 998
Fragments arranged by size:
998 193 105
BsaHI Gr'CG_yC
Cuts at: 0 5 283 1296
Size: 5 278 1013
Fragments arranged by size:
1013 278 5
BsaWI w'CCGG_w
Cuts at: 0 81 202 551 1296
Size: 81 121 349 745
Fragments arranged by size:
745 349 121 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 541 571 1296
Size: 541 30 725
Fragments arranged by size:
725 541 30
BseYI C'CCAG_C
Cuts at: 0 95 792 1185 1296
Size: 95 697 393 111
Fragments arranged by size:
697 393 111 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 919 1296
Size: 190 729 377
Fragments arranged by size:
729 377 190
BsmI GAATG_Cn'
Cuts at: 0 129 1296
Size: 129 1167
Bsp1286I G_dGCh'C
Cuts at: 0 257 356 543 782 949 969 1296
Size: 257 99 187 239 167 20 327
Fragments arranged by size:
327 257 239 187 167 99 20
BspEI T'CCGG_A
Cuts at: 0 81 1296
Size: 81 1215
BspHI T'CATG_A
Cuts at: 0 1109 1296
Size: 1109 187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 46 817 1296
Size: 46 771 479
Fragments arranged by size:
771 479 46
BsrDI GCAATG_nn'
Cuts at: 0 255 1296
Size: 255 1041
BsrFI r'CCGG_y
Cuts at: 0 202 551 1296
Size: 202 349 745
Fragments arranged by size:
745 349 202
BstAPI GCAn_nnn'nTGC
Cuts at: 0 593 1296
Size: 593 703
BstXI CCAn_nnnn'nTGG
Cuts at: 0 147 1296
Size: 147 1149
BtsI GCAGTG_nn'
Cuts at: 0 26 949 1296
Size: 26 923 347
Fragments arranged by size:
923 347 26
DraIII CAC_nnn'GTG
Cuts at: 0 436 1296
Size: 436 860
EaeI y'GGCC_r
Cuts at: 0 641 1050 1158 1296
Size: 641 409 108 138
Fragments arranged by size:
641 409 138 108
EarI CTCTTCn'nnn_
Cuts at: 0 343 940 1063 1296
Size: 343 597 123 233
Fragments arranged by size:
597 343 233 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 198 1108 1296
Size: 198 910 188
Fragments arranged by size:
910 198 188
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 217 340 994 1296
Size: 162 55 123 654 302
Fragments arranged by size:
654 302 162 123 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 973 1120 1296
Size: 973 147 176
Fragments arranged by size:
973 176 147
EcoO109I rG'GnC_Cy
Cuts at: 0 210 324 965 966 1296
Size: 210 114 641 1 330
Fragments arranged by size:
641 330 210 114 1
HaeII r_GCGC'y
Cuts at: 0 286 444 1296
Size: 286 158 852
Fragments arranged by size:
852 286 158
HindIII A'AGCT_T
Cuts at: 0 233 682 1296
Size: 233 449 614
Fragments arranged by size:
614 449 233
KasI G'GCGC_C
Cuts at: 0 282 1296
Size: 282 1014
KpnI G_GTAC'C
Cuts at: 0 154 1296
Size: 154 1142
MscI TGG'CCA
Cuts at: 0 643 1052 1160 1296
Size: 643 409 108 136
Fragments arranged by size:
643 409 136 108
MslI CAynn'nnrTG
Cuts at: 0 313 634 673 1108 1174 1296
Size: 313 321 39 435 66 122
Fragments arranged by size:
435 321 313 122 66 39
MspA1I CmG'CkG
Cuts at: 0 590 770 1296
Size: 590 180 526
Fragments arranged by size:
590 526 180
NarI GG'CG_CC
Cuts at: 0 283 1296
Size: 283 1013
NspI r_CATG'y
Cuts at: 0 756 1049 1296
Size: 756 293 247
Fragments arranged by size:
756 293 247
PflMI CCAn_nnn'nTGG
Cuts at: 0 14 639 1296
Size: 14 625 657
Fragments arranged by size:
657 625 14
PspOMI G'GGCC_C
Cuts at: 0 965 1296
Size: 965 331
PstI C_TGCA'G
Cuts at: 0 389 1296
Size: 389 907
PvuII CAG'CTG
Cuts at: 0 590 1296
Size: 590 706
SapI GCTCTTCn'nnn_
Cuts at: 0 343 1063 1296
Size: 343 720 233
Fragments arranged by size:
720 343 233
ScaI AGT'ACT
Cuts at: 0 175 1296
Size: 175 1121
SfcI C'TryA_G
Cuts at: 0 385 1296
Size: 385 911
SfoI GGC'GCC
Cuts at: 0 284 1296
Size: 284 1012
SmlI C'TyrA_G
Cuts at: 0 610 1296
Size: 610 686
StuI AGG'CCT
Cuts at: 0 262 649 812 1126 1296
Size: 262 387 163 314 170
Fragments arranged by size:
387 314 262 170 163
StyI C'CwwG_G
Cuts at: 0 48 257 644 1296
Size: 48 209 387 652
Fragments arranged by size:
652 387 209 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 558 646 1296
Size: 558 88 650
Fragments arranged by size:
650 558 88
TatI w'GTAC_w
Cuts at: 0 173 1296
Size: 173 1123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 671 703 1296
Size: 671 32 593
Fragments arranged by size:
671 593 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 636 1296
Size: 636 660
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AgeI AleI
AlfI AloI AlwNI ApaI AvrII BaeI BanI BanII
BbeI BbsI BlpI Bme1580I BmrI BpmI BsaI BsaHI
BsaWI BsaXI BseYI BsgI BsmI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BstAPI BstXI BtsI DraIII EaeI
EarI EciI Eco57MI EcoNI EcoO109I HaeII HindIII KasI
KpnI MscI MslI MspA1I NarI NspI PflMI PspOMI
PstI PvuII SapI ScaI SfcI SfoI SmlI StuI
StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AclI AflIII AhdI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BseRI BsiEI BsiHKAI BsiWI BsmBI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HpaI
MfeI MluI MmeI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI XmaI XmnI ZraI