Restriction map for
pFN21AE6345
(Linear) (Six-base) MAPSORT of: KIEE6345.seq Check: 5794 from: 1 to: 1107
KIEE6345.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AccI GT'mk_AC
Cuts at: 0 269 374 767 1107
Size: 269 105 393 340
Fragments arranged by size:
393 340 269 105
Acc65I G'GTAC_C
Cuts at: 0 322 1107
Size: 322 785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 1107
Size: 178 929
AflIII A'CryG_T
Cuts at: 0 272 556 1107
Size: 272 284 551
Fragments arranged by size:
551 284 272
AlwNI CAG_nnn'CTG
Cuts at: 0 27 169 226 627 921 1107
Size: 27 142 57 401 294 186
Fragments arranged by size:
401 294 186 142 57 27
ApaI G_GGCC'C
Cuts at: 0 832 1107
Size: 832 275
ApaLI G'TGCA_C
Cuts at: 0 460 1107
Size: 460 647
AvaI C'yCGr_G
Cuts at: 0 106 725 1107
Size: 106 619 382
Fragments arranged by size:
619 382 106
BanI G'GyrC_C
Cuts at: 0 322 467 853 1107
Size: 322 145 386 254
Fragments arranged by size:
386 322 254 145
BanII G_rGCy'C
Cuts at: 0 101 240 832 1037 1107
Size: 101 139 592 205 70
Fragments arranged by size:
592 205 139 101 70
BbeI G_GCGC'C
Cuts at: 0 857 1107
Size: 857 250
BbsI GAAGACnn'nnnn_
Cuts at: 0 166 446 1107
Size: 166 280 661
Fragments arranged by size:
661 280 166
BglI GCCn_nnn'nGGC
Cuts at: 0 661 876 1025 1107
Size: 661 215 149 82
Fragments arranged by size:
661 215 149 82
BlpI GC'TnA_GC
Cuts at: 0 1013 1107
Size: 1013 94
Bme1580I G_kGCm'C
Cuts at: 0 464 564 832 1107
Size: 464 100 268 275
Fragments arranged by size:
464 275 268 100
BmgBI CAC'GTC
Cuts at: 0 198 273 557 1107
Size: 198 75 284 550
Fragments arranged by size:
550 284 198 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 661 1107
Size: 661 446
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 532 754 1107
Size: 169 363 222 353
Fragments arranged by size:
363 353 222 169
BsaAI yAC'GTr
Cuts at: 0 72 714 1107
Size: 72 642 393
Fragments arranged by size:
642 393 72
BsaHI Gr'CG_yC
Cuts at: 0 854 1107
Size: 854 253
BsaWI w'CCGG_w
Cuts at: 0 355 1107
Size: 355 752
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 56 86 227 257 1107
Size: 56 30 141 30 850
Fragments arranged by size:
850 141 56 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 1107
Size: 166 941
BseYI C'CCAG_C
Cuts at: 0 259 1107
Size: 259 848
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 370 1107
Size: 337 33 737
Fragments arranged by size:
737 337 33
BsiHKAI G_wGCw'C
Cuts at: 0 71 464 941 1107
Size: 71 393 477 166
Fragments arranged by size:
477 393 166 71
BsmI GAATG_Cn'
Cuts at: 0 86 1107
Size: 86 1021
Bsp1286I G_dGCh'C
Cuts at: 0 71 101 240 464 564 832 941 1037
Size: 71 30 139 224 100 268 109 96
Cuts at: 1037 1107
Size: 70
Fragments arranged by size:
268 224 139 109 100 96 71 70
30
BspHI T'CATG_A
Cuts at: 0 602 1107
Size: 602 505
BsrDI GCAATG_nn'
Cuts at: 0 702 862 1107
Size: 702 160 245
Fragments arranged by size:
702 245 160
BssSI C'ACGA_G
Cuts at: 0 1044 1107
Size: 1044 63
BstAPI GCAn_nnn'nTGC
Cuts at: 0 75 921 1107
Size: 75 846 186
Fragments arranged by size:
846 186 75
BstEII G'GTnAC_C
Cuts at: 0 415 1107
Size: 415 692
BstXI CCAn_nnnn'nTGG
Cuts at: 0 259 387 1107
Size: 259 128 720
Fragments arranged by size:
720 259 128
BstZ17I GTA'TAC
Cuts at: 0 768 1107
Size: 768 339
BtgI C'CryG_G
Cuts at: 0 534 572 974 1107
Size: 534 38 402 133
Fragments arranged by size:
534 402 133 38
BtsI GCAGTG_nn'
Cuts at: 0 464 922 1107
Size: 464 458 185
Fragments arranged by size:
464 458 185
DraIII CAC_nnn'GTG
Cuts at: 0 75 1107
Size: 75 1032
EaeI y'GGCC_r
Cuts at: 0 2 230 494 678 759 928 971 977
Size: 2 228 264 184 81 169 43 6
Cuts at: 977 1107
Size: 130
Fragments arranged by size:
264 228 184 169 130 81 43 6
2
EarI CTCTTCn'nnn_
Cuts at: 0 652 1107
Size: 652 455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 178 532 754 1107
Size: 169 9 354 222 353
Fragments arranged by size:
354 353 222 169 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 426 1107
Size: 426 681
EcoO109I rG'GnC_Cy
Cuts at: 0 139 166 400 489 624 829 1107
Size: 139 27 234 89 135 205 278
Fragments arranged by size:
278 234 205 139 135 89 27
HaeII r_GCGC'y
Cuts at: 0 857 1020 1107
Size: 857 163 87
Fragments arranged by size:
857 163 87
HincII GTy'rAC
Cuts at: 0 312 1107
Size: 312 795
KasI G'GCGC_C
Cuts at: 0 853 1107
Size: 853 254
KpnI G_GTAC'C
Cuts at: 0 326 1107
Size: 326 781
MscI TGG'CCA
Cuts at: 0 979 1107
Size: 979 128
MspA1I CmG'CkG
Cuts at: 0 93 226 741 1107
Size: 93 133 515 366
Fragments arranged by size:
515 366 133 93
NarI GG'CG_CC
Cuts at: 0 854 1107
Size: 854 253
PflMI CCAn_nnn'nTGG
Cuts at: 0 27 135 216 1076 1107
Size: 27 108 81 860 31
Fragments arranged by size:
860 108 81 31 27
PfoI T'CCnGG_A
Cuts at: 0 509 1107
Size: 509 598
PmlI CAC'GTG
Cuts at: 0 72 714 1107
Size: 72 642 393
Fragments arranged by size:
642 393 72
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1024 1056 1107
Size: 1024 32 51
Fragments arranged by size:
1024 51 32
PpuMI rG'GwC_Cy
Cuts at: 0 139 1107
Size: 139 968
PspOMI G'GGCC_C
Cuts at: 0 828 1107
Size: 828 279
PstI C_TGCA'G
Cuts at: 0 92 917 1107
Size: 92 825 190
Fragments arranged by size:
825 190 92
PvuII CAG'CTG
Cuts at: 0 226 1107
Size: 226 881
SanDI GG'GwC_CC
Cuts at: 0 139 1107
Size: 139 968
SapI GCTCTTCn'nnn_
Cuts at: 0 652 1107
Size: 652 455
SexAI A'CCwGG_T
Cuts at: 0 35 1107
Size: 35 1072
SfcI C'TryA_G
Cuts at: 0 88 426 792 913 1107
Size: 88 338 366 121 194
Fragments arranged by size:
366 338 194 121 88
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 661 1107
Size: 661 446
SfoI GGC'GCC
Cuts at: 0 855 1107
Size: 855 252
SmaI CCC'GGG
Cuts at: 0 727 1107
Size: 727 380
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 56 88 227 259 519 551 1107
Size: 56 32 139 32 260 32 556
Fragments arranged by size:
556 260 139 56 32 32 32
XmaI C'CCGG_G
Cuts at: 0 725 1107
Size: 725 382
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI
BplI BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BssSI BstAPI
BstEII BstXI BstZ17I BtgI BtsI DraIII EaeI EarI
Eco57MI EcoNI EcoO109I HaeII HincII KasI KpnI MscI
MspA1I NarI PflMI PfoI PmlI PpiI PpuMI PspOMI
PstI PvuII SanDI SapI SexAI SfcI SfiI SfoI
SmaI TstI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmrI
BmtI Bpu10I BpuEI BsaI BsaBI BsiEI BsiWI BsmBI
BspEI BspMI BsrBI BsrFI BsrGI BssHII BstBI BstYI
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HindIII HpaI MfeI MluI MmeI MslI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SbfI ScaI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmnI
ZraI