Restriction map for
pFN21AE6344
(Linear) (Six-base) MAPSORT of: KIEE6344.seq Check: 8961 from: 1 to: 1065
KIEE6344.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 887 1065
Size: 887 178
AatII G_ACGT'C
Cuts at: 0 34 1065
Size: 34 1031
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 281 474 847 1065
Size: 37 244 193 373 218
Fragments arranged by size:
373 244 218 193 37
AfeI AGC'GCT
Cuts at: 0 481 1065
Size: 481 584
AleI CACnn'nnGTG
Cuts at: 0 1036 1065
Size: 1036 29
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 433 1065
Size: 433 632
ApoI r'AATT_y
Cuts at: 0 80 346 1065
Size: 80 266 719
Fragments arranged by size:
719 266 80
AvaI C'yCGr_G
Cuts at: 0 44 1065
Size: 44 1021
BanI G'GyrC_C
Cuts at: 0 61 1065
Size: 61 1004
BanII G_rGCy'C
Cuts at: 0 963 1065
Size: 963 102
BbeI G_GCGC'C
Cuts at: 0 65 1065
Size: 65 1000
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 844 878 1065
Size: 844 34 187
Fragments arranged by size:
844 187 34
Bme1580I G_kGCm'C
Cuts at: 0 629 1065
Size: 629 436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1065
Size: 529 536
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 835 1065
Size: 773 62 230
Fragments arranged by size:
773 230 62
BsaBI GATnn'nnATC
Cuts at: 0 616 1065
Size: 616 449
BsaHI Gr'CG_yC
Cuts at: 0 31 62 1065
Size: 31 31 1003
Fragments arranged by size:
1003 31 31
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 502 532 1065
Size: 502 30 533
Fragments arranged by size:
533 502 30
BseYI C'CCAG_C
Cuts at: 0 65 1065
Size: 65 1000
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 1065
Size: 115 950
BsiHKAI G_wGCw'C
Cuts at: 0 446 963 1065
Size: 446 517 102
Fragments arranged by size:
517 446 102
BsmI GAATG_Cn'
Cuts at: 0 720 926 1065
Size: 720 206 139
Fragments arranged by size:
720 206 139
Bsp1286I G_dGCh'C
Cuts at: 0 446 629 963 1065
Size: 446 183 334 102
Fragments arranged by size:
446 334 183 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 602 887 1065
Size: 602 285 178
Fragments arranged by size:
602 285 178
BsrDI GCAATG_nn'
Cuts at: 0 375 1065
Size: 375 690
BsrGI T'GTAC_A
Cuts at: 0 404 518 1065
Size: 404 114 547
Fragments arranged by size:
547 404 114
BstYI r'GATC_y
Cuts at: 0 666 1052 1065
Size: 666 386 13
Fragments arranged by size:
666 386 13
BtgI C'CryG_G
Cuts at: 0 844 1065
Size: 844 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 529 1065
Size: 529 536
BtsI GCAGTG_nn'
Cuts at: 0 96 103 550 1065
Size: 96 7 447 515
Fragments arranged by size:
515 447 96 7
ClaI AT'CG_AT
Cuts at: 0 620 1065
Size: 620 445
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 785 820 1065
Size: 785 35 245
Fragments arranged by size:
785 245 35
DraIII CAC_nnn'GTG
Cuts at: 0 609 1065
Size: 609 456
DrdI GACnn_nn'nnGTC
Cuts at: 0 36 1065
Size: 36 1029
EarI CTCTTCn'nnn_
Cuts at: 0 565 1065
Size: 565 500
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 30 663 1065
Size: 30 633 402
Fragments arranged by size:
633 402 30
EcoICRI GAG'CTC
Cuts at: 0 961 1065
Size: 961 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 281 474 529 847 1065
Size: 37 244 193 55 318 218
Fragments arranged by size:
318 244 218 193 55 37
EcoRI G'AATT_C
Cuts at: 0 80 1065
Size: 80 985
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 232 747 1065
Size: 232 515 318
Fragments arranged by size:
515 318 232
HaeII r_GCGC'y
Cuts at: 0 65 483 1065
Size: 65 418 582
Fragments arranged by size:
582 418 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 261 293 1065
Size: 261 32 772
Fragments arranged by size:
772 261 32
KasI G'GCGC_C
Cuts at: 0 61 1065
Size: 61 1004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 1065
Size: 455 610
MslI CAynn'nnrTG
Cuts at: 0 89 622 1036 1065
Size: 89 533 414 29
Fragments arranged by size:
533 414 89 29
NarI GG'CG_CC
Cuts at: 0 62 1065
Size: 62 1003
PasI CC'CwG_GG
Cuts at: 0 1021 1065
Size: 1021 44
PfoI T'CCnGG_A
Cuts at: 0 506 941 1065
Size: 506 435 124
Fragments arranged by size:
506 435 124
PstI C_TGCA'G
Cuts at: 0 395 425 1065
Size: 395 30 640
Fragments arranged by size:
640 395 30
SacI G_AGCT'C
Cuts at: 0 963 1065
Size: 963 102
SapI GCTCTTCn'nnn_
Cuts at: 0 565 1065
Size: 565 500
ScaI AGT'ACT
Cuts at: 0 427 1065
Size: 427 638
SexAI A'CCwGG_T
Cuts at: 0 605 1065
Size: 605 460
SfcI C'TryA_G
Cuts at: 0 182 333 391 421 700 1065
Size: 182 151 58 30 279 365
Fragments arranged by size:
365 279 182 151 58 30
SfoI GGC'GCC
Cuts at: 0 63 1065
Size: 63 1002
SmaI CCC'GGG
Cuts at: 0 46 1065
Size: 46 1019
SmlI C'TyrA_G
Cuts at: 0 788 850 1065
Size: 788 62 215
Fragments arranged by size:
788 215 62
StyI C'CwwG_G
Cuts at: 0 437 1065
Size: 437 628
TatI w'GTAC_w
Cuts at: 0 199 404 425 518 1013 1065
Size: 199 205 21 93 495 52
Fragments arranged by size:
495 205 199 93 52 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 912 944 1065
Size: 912 32 121
Fragments arranged by size:
912 121 32
Tth111I GACn'n_nGTC
Cuts at: 0 976 1065
Size: 976 89
XmaI C'CCGG_G
Cuts at: 0 44 1065
Size: 44 1021
ZraI GAC'GTC
Cuts at: 0 32 1065
Size: 32 1033
Enzymes that do cut:
AarI AatII AcuI AfeI AleI AlfI ApoI AvaI
BanI BanII BbeI BcgI Bme1580I BpmI BpuEI BsaBI
BsaHI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BsrGI BstYI BtgI BtgZI BtsI ClaI CspCI
DraIII DrdI EarI EciI EcoICRI Eco57MI EcoRI FalI
HaeII Hin4I KasI MmeI MslI NarI PasI PfoI
PstI SacI SapI ScaI SexAI SfcI SfoI SmaI
SmlI StyI TatI TstI Tth111I XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI
BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I DraI EaeI EagI EcoNI EcoO109I EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MscI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII XbaI XcmI XhoI XmnI