Restriction map for
pFN21AE6341
(Linear) (Six-base) MAPSORT of: KIEE6341.seq Check: 1842 from: 1 to: 1272
KIEE6341.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 111 1272
Size: 111 1161
Acc65I G'GTAC_C
Cuts at: 0 869 1272
Size: 869 403
AfeI AGC'GCT
Cuts at: 0 693 1272
Size: 693 579
AflIII A'CryG_T
Cuts at: 0 539 1272
Size: 539 733
AlwNI CAG_nnn'CTG
Cuts at: 0 274 942 1010 1272
Size: 274 668 68 262
Fragments arranged by size:
668 274 262 68
ApaI G_GGCC'C
Cuts at: 0 249 1272
Size: 249 1023
ApaLI G'TGCA_C
Cuts at: 0 952 1272
Size: 952 320
AvaI C'yCGr_G
Cuts at: 0 227 249 415 887 925 1272
Size: 227 22 166 472 38 347
Fragments arranged by size:
472 347 227 166 38 22
BanI G'GyrC_C
Cuts at: 0 123 588 869 1272
Size: 123 465 281 403
Fragments arranged by size:
465 403 281 123
BanII G_rGCy'C
Cuts at: 0 110 249 779 1272
Size: 110 139 530 493
Fragments arranged by size:
530 493 139 110
BbsI GAAGACnn'nnnn_
Cuts at: 0 837 1272
Size: 837 435
BglI GCCn_nnn'nGGC
Cuts at: 0 643 862 1272
Size: 643 219 410
Fragments arranged by size:
643 410 219
BglII A'GATC_T
Cuts at: 0 1263 1272
Size: 1263 9
Bme1580I G_kGCm'C
Cuts at: 0 249 593 956 1272
Size: 249 344 363 316
Fragments arranged by size:
363 344 316 249
BmgBI CAC'GTC
Cuts at: 0 951 1272
Size: 951 321
BmrI ACTGGGnnnn_n'
Cuts at: 0 990 1272
Size: 990 282
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 242 1272
Size: 242 1030
Bpu10I CC'TnA_GC
Cuts at: 0 1075 1272
Size: 1075 197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 1272
Size: 682 590
BsaAI yAC'GTr
Cuts at: 0 540 1272
Size: 540 732
BsaWI w'CCGG_w
Cuts at: 0 449 1272
Size: 449 823
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 67 1272
Size: 64 3 1205
Fragments arranged by size:
1205 64 3
BseYI C'CCAG_C
Cuts at: 0 116 434 1272
Size: 116 318 838
Fragments arranged by size:
838 318 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 1272
Size: 430 842
BsiEI CG_ry'CG
Cuts at: 0 409 720 1272
Size: 409 311 552
Fragments arranged by size:
552 409 311
BsiHKAI G_wGCw'C
Cuts at: 0 110 578 626 821 956 1272
Size: 110 468 48 195 135 316
Fragments arranged by size:
468 316 195 135 110 48
BsmBI CGTCTCn'nnnn_
Cuts at: 0 745 1272
Size: 745 527
Bsp1286I G_dGCh'C
Cuts at: 0 110 249 578 593 626 779 821 956
Size: 110 139 329 15 33 153 42 135
Cuts at: 956 1272
Size: 316
Fragments arranged by size:
329 316 153 139 135 110 42 33
15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 111 272 950 1272
Size: 111 161 678 322
Fragments arranged by size:
678 322 161 111
BsrFI r'CCGG_y
Cuts at: 0 598 637 904 956 995 1124 1272
Size: 598 39 267 52 39 129 148
Fragments arranged by size:
598 267 148 129 52 39 39
BssHII G'CGCG_C
Cuts at: 0 1088 1272
Size: 1088 184
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1010 1272
Size: 1010 262
BstYI r'GATC_y
Cuts at: 0 1263 1272
Size: 1263 9
BtgI C'CryG_G
Cuts at: 0 962 1093 1272
Size: 962 131 179
Fragments arranged by size:
962 179 131
BtsI GCAGTG_nn'
Cuts at: 0 653 1272
Size: 653 619
DraIII CAC_nnn'GTG
Cuts at: 0 573 1272
Size: 573 699
EaeI y'GGCC_r
Cuts at: 0 377 406 429 635 1272
Size: 377 29 23 206 637
Fragments arranged by size:
637 377 206 29 23
EagI C'GGCC_G
Cuts at: 0 406 1272
Size: 406 866
EarI CTCTTCn'nnn_
Cuts at: 0 48 85 1272
Size: 48 37 1187
Fragments arranged by size:
1187 48 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 310 786 1272
Size: 310 476 486
Fragments arranged by size:
486 476 310
EcoICRI GAG'CTC
Cuts at: 0 108 1272
Size: 108 1164
EcoNI CCTnn'n_nnAGG
Cuts at: 0 783 1272
Size: 783 489
EcoO109I rG'GnC_Cy
Cuts at: 0 96 245 246 412 894 1272
Size: 96 149 1 166 482 378
Fragments arranged by size:
482 378 166 149 96 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 780 1272
Size: 780 492
FseI GG_CCGG'CC
Cuts at: 0 641 1272
Size: 641 631
HaeII r_GCGC'y
Cuts at: 0 274 330 695 705 1272
Size: 274 56 365 10 567
Fragments arranged by size:
567 365 274 56 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1114 1146 1272
Size: 1114 32 126
Fragments arranged by size:
1114 126 32
KpnI G_GTAC'C
Cuts at: 0 873 1272
Size: 873 399
MfeI C'AATT_G
Cuts at: 0 609 1272
Size: 609 663
MslI CAynn'nnrTG
Cuts at: 0 757 1272
Size: 757 515
MspA1I CmG'CkG
Cuts at: 0 294 361 434 1095 1272
Size: 294 67 73 661 177
Fragments arranged by size:
661 294 177 73 67
NaeI GCC'GGC
Cuts at: 0 600 639 997 1272
Size: 600 39 358 275
Fragments arranged by size:
600 358 275 39
NgoMIV G'CCGG_C
Cuts at: 0 598 637 995 1272
Size: 598 39 358 277
Fragments arranged by size:
598 358 277 39
NspI r_CATG'y
Cuts at: 0 39 1272
Size: 39 1233
PmlI CAC'GTG
Cuts at: 0 540 1272
Size: 540 732
PpuMI rG'GwC_Cy
Cuts at: 0 96 412 1272
Size: 96 316 860
Fragments arranged by size:
860 316 96
PspOMI G'GGCC_C
Cuts at: 0 245 1272
Size: 245 1027
PstI C_TGCA'G
Cuts at: 0 69 95 299 1006 1272
Size: 69 26 204 707 266
Fragments arranged by size:
707 266 204 69 26
PvuII CAG'CTG
Cuts at: 0 361 1272
Size: 361 911
RsrII CG'GwC_CG
Cuts at: 0 902 1272
Size: 902 370
SacI G_AGCT'C
Cuts at: 0 110 1272
Size: 110 1162
SacII CC_GC'GG
Cuts at: 0 1096 1272
Size: 1096 176
SanDI GG'GwC_CC
Cuts at: 0 412 1272
Size: 412 860
SbfI CC_TGCA'GG
Cuts at: 0 95 1272
Size: 95 1177
ScaI AGT'ACT
Cuts at: 0 1171 1272
Size: 1171 101
SfcI C'TryA_G
Cuts at: 0 65 91 295 1002 1272
Size: 65 26 204 707 270
Fragments arranged by size:
707 270 204 65 26
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 643 862 1272
Size: 643 219 410
Fragments arranged by size:
643 410 219
SmaI CCC'GGG
Cuts at: 0 417 1272
Size: 417 855
SmlI C'TyrA_G
Cuts at: 0 697 1272
Size: 697 575
StuI AGG'CCT
Cuts at: 0 71 487 1272
Size: 71 416 785
Fragments arranged by size:
785 416 71
StyI C'CwwG_G
Cuts at: 0 151 593 1272
Size: 151 442 679
Fragments arranged by size:
679 442 151
TatI w'GTAC_w
Cuts at: 0 1169 1272
Size: 1169 103
Tth111I GACn'n_nGTC
Cuts at: 0 988 1272
Size: 988 284
XmaI C'CCGG_G
Cuts at: 0 415 1272
Size: 415 857
Enzymes that do cut:
AarI Acc65I AfeI AflIII AlwNI ApaI ApaLI AvaI
BanI BanII BbsI BglI BglII Bme1580I BmgBI BmrI
BplI Bpu10I BpuEI BsaAI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssHII BstAPI
BstYI BtgI BtsI DraIII EaeI EagI EarI EciI
EcoICRI EcoNI EcoO109I FalI FseI HaeII Hin4I KpnI
MfeI MslI MspA1I NaeI NgoMIV NspI PmlI PpuMI
PspOMI PstI PvuII RsrII SacI SacII SanDI SbfI
ScaI SfcI SfiI SmaI SmlI StuI StyI TatI
Tth111I XmaI
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI
BmtI BpmI BsaI BsaBI BsaHI BsaXI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
Eco57MI EcoRI EcoRV FspI FspAI HincII HindIII HpaI
KasI MluI MmeI MscI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI SalI
SapI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI XbaI XcmI XhoI
XmnI ZraI