(Linear) (Six-base) MAPSORT of: KIEE6338.seq  Check: 6999  from: 1  to: 939



KIEE6338.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:30  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     939 
   Size:         41     898
 
AhdI GACnn_n'nnGTC

Cuts at:      0       8     939 
   Size:          8     931
 
ApaLI G'TGCA_C

Cuts at:      0     369     939 
   Size:        369     570
 
ApoI r'AATT_y

Cuts at:      0     640     939 
   Size:        640     299
 
AvaI C'yCGr_G

Cuts at:      0     695     939 
   Size:        695     244
 
BamHI G'GATC_C

Cuts at:      0     774     939 
   Size:        774     165
 
BanII G_rGCy'C

Cuts at:      0     279     939 
   Size:        279     660
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     808     939 
   Size:        808     131
 
BbvCI CC'TCA_GC

Cuts at:      0     542     939 
   Size:        542     397
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     527     939 
   Size:        527     412
 
Bme1580I G_kGCm'C

Cuts at:      0     373     939 
   Size:        373     566
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     823     939 
   Size:        823     116
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     293     939 
   Size:        293     646
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     939 
   Size:        157     782
 
Bpu10I CC'TnA_GC

Cuts at:      0     542     939 
   Size:        542     397
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     263     403     533     939 
   Size:        263     140     130     406

  Fragments arranged by size: 

                 406     263     140     130
 
BsaWI w'CCGG_w

Cuts at:      0      76     939 
   Size:         76     863
 
BsiHKAI G_wGCw'C

Cuts at:      0     373     939 
   Size:        373     566
 
Bsp1286I G_dGCh'C

Cuts at:      0     279     373     939 
   Size:        279      94     566

  Fragments arranged by size: 

                 566     279      94
 
BsrDI GCAATG_nn'

Cuts at:      0      14     939 
   Size:         14     925
 
BstYI r'GATC_y

Cuts at:      0     774     939 
   Size:        774     165
 
BtgI C'CryG_G

Cuts at:      0      60     939 
   Size:         60     879
 
BtsI GCAGTG_nn'

Cuts at:      0     176     760     939 
   Size:        176     584     179

  Fragments arranged by size: 

                 584     179     176
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     833     939 
   Size:        833     106
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     157     939 
   Size:         41     116     782

  Fragments arranged by size: 

                 782     116      41
 
EcoRV GAT'ATC

Cuts at:      0     570     939 
   Size:        570     369
 
HaeII r_GCGC'y

Cuts at:      0     116     939 
   Size:        116     823
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      26     459     491     554     586     939 
   Size:         26     433      32      63      32     353

  Fragments arranged by size: 

                 433     353      63      32      32      26
 
HindIII A'AGCT_T

Cuts at:      0     878     939 
   Size:        878      61
 
MslI CAynn'nnrTG

Cuts at:      0      65     626     939 
   Size:         65     561     313

  Fragments arranged by size: 

                 561     313      65
 
NcoI C'CATG_G

Cuts at:      0      60     939 
   Size:         60     879
 
NdeI CA'TA_TG

Cuts at:      0     261     939 
   Size:        261     678
 
NspI r_CATG'y

Cuts at:      0     604     939 
   Size:        604     335
 
PfoI T'CCnGG_A

Cuts at:      0     391     939 
   Size:        391     548
 
PstI C_TGCA'G

Cuts at:      0     743     939 
   Size:        743     196
 
SfcI C'TryA_G

Cuts at:      0     739     939 
   Size:        739     200
 
SmaI CCC'GGG

Cuts at:      0     697     939 
   Size:        697     242
 
SmlI C'TyrA_G

Cuts at:      0     278     382     548     939 
   Size:        278     104     166     391

  Fragments arranged by size: 

                 391     278     166     104
 
StyI C'CwwG_G

Cuts at:      0      60     939 
   Size:         60     879
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     360     519     939 
   Size:        360     159     420

  Fragments arranged by size: 

                 420     360     159
 
XmaI C'CCGG_G

Cuts at:      0     695     939 
   Size:        695     244
 

 Enzymes that do cut:

     AcuI     AhdI    ApaLI     ApoI     AvaI    BamHI    BanII     BbsI
    BbvCI    BciVI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
    BsaWI  BsiHKAI Bsp1286I    BsrDI    BstYI     BtgI     BtsI     EciI
  Eco57MI    EcoRV    HaeII    Hin4I  HindIII     MslI     NcoI     NdeI
     NspI     PfoI     PstI     SfcI     SmaI     SmlI     StyI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI    AvrII     BaeI     BanI     BbeI     BcgI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmnI     ZraI