Restriction map for
pFN21AE6335
(Linear) (Six-base) MAPSORT of: KIEE6335.seq Check: 4267 from: 1 to: 1215
KIEE6335.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 1215
Size: 625 590
AfeI AGC'GCT
Cuts at: 0 124 503 1215
Size: 124 379 712
Fragments arranged by size:
712 379 124
AflIII A'CryG_T
Cuts at: 0 653 1215
Size: 653 562
AleI CACnn'nnGTG
Cuts at: 0 103 1215
Size: 103 1112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 55 1215
Size: 55 1160
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 886 918 1215
Size: 886 32 297
Fragments arranged by size:
886 297 32
AlwNI CAG_nnn'CTG
Cuts at: 0 495 763 1215
Size: 495 268 452
Fragments arranged by size:
495 452 268
AseI AT'TA_AT
Cuts at: 0 187 1215
Size: 187 1028
AvaI C'yCGr_G
Cuts at: 0 872 1215
Size: 872 343
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 454 487 1215
Size: 454 33 728
Fragments arranged by size:
728 454 33
BanI G'GyrC_C
Cuts at: 0 67 660 1215
Size: 67 593 555
Fragments arranged by size:
593 555 67
BbeI G_GCGC'C
Cuts at: 0 71 1215
Size: 71 1144
BbsI GAAGACnn'nnnn_
Cuts at: 0 704 1115 1215
Size: 704 411 100
Fragments arranged by size:
704 411 100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 528 562 1077 1111 1215
Size: 528 34 515 34 104
Fragments arranged by size:
528 515 104 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 634 751 772 1215
Size: 634 117 21 443
Fragments arranged by size:
634 443 117 21
BglII A'GATC_T
Cuts at: 0 20 1181 1215
Size: 20 1161 34
Fragments arranged by size:
1161 34 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 1134 1215
Size: 1134 81
BmtI G_CTAG'C
Cuts at: 0 1102 1215
Size: 1102 113
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 117 1215
Size: 117 1098
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 338 1138 1215
Size: 100 238 800 77
Fragments arranged by size:
800 238 100 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 115 1215
Size: 100 15 1100
Fragments arranged by size:
1100 100 15
BsaHI Gr'CG_yC
Cuts at: 0 68 1215
Size: 68 1147
BsaWI w'CCGG_w
Cuts at: 0 455 615 842 983 996 1215
Size: 455 160 227 141 13 219
Fragments arranged by size:
455 227 219 160 141 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 379 409 886 916 1215
Size: 379 30 477 30 299
Fragments arranged by size:
477 379 299 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 97 817 1015 1215
Size: 28 69 720 198 200
Fragments arranged by size:
720 200 198 69 28
BseYI C'CCAG_C
Cuts at: 0 975 1071 1215
Size: 975 96 144
Fragments arranged by size:
975 144 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 31 1087 1215
Size: 28 3 1056 128
Fragments arranged by size:
1056 128 28 3
BsiHKAI G_wGCw'C
Cuts at: 0 144 288 1215
Size: 144 144 927
Fragments arranged by size:
927 144 144
BsmI GAATG_Cn'
Cuts at: 0 546 960 1215
Size: 546 414 255
Fragments arranged by size:
546 414 255
Bsp1286I G_dGCh'C
Cuts at: 0 144 288 1215
Size: 144 144 927
Fragments arranged by size:
927 144 144
BspEI T'CCGG_A
Cuts at: 0 455 615 842 996 1215
Size: 455 160 227 154 219
Fragments arranged by size:
455 227 219 160 154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 398 1215
Size: 398 817
BsrDI GCAATG_nn'
Cuts at: 0 551 1093 1215
Size: 551 542 122
Fragments arranged by size:
551 542 122
BstYI r'GATC_y
Cuts at: 0 20 159 839 1181 1215
Size: 20 139 680 342 34
Fragments arranged by size:
680 342 139 34 20
Bsu36I CC'TnA_GG
Cuts at: 0 665 1215
Size: 665 550
BtgI C'CryG_G
Cuts at: 0 416 671 779 1215
Size: 416 255 108 436
Fragments arranged by size:
436 416 255 108
DraIII CAC_nnn'GTG
Cuts at: 0 1142 1215
Size: 1142 73
DrdI GACnn_nn'nnGTC
Cuts at: 0 259 1215
Size: 259 956
EaeI y'GGCC_r
Cuts at: 0 726 1215
Size: 726 489
EarI CTCTTCn'nnn_
Cuts at: 0 233 725 1215
Size: 233 492 490
Fragments arranged by size:
492 490 233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 338 625 1138 1215
Size: 100 238 287 513 77
Fragments arranged by size:
513 287 238 100 77
EcoNI CCTnn'n_nnAGG
Cuts at: 0 204 1215
Size: 204 1011
EcoO109I rG'GnC_Cy
Cuts at: 0 636 1165 1215
Size: 636 529 50
Fragments arranged by size:
636 529 50
HaeII r_GCGC'y
Cuts at: 0 71 126 505 794 1215
Size: 71 55 379 289 421
Fragments arranged by size:
421 379 289 71 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1137 1169 1191 1215
Size: 1137 32 22 24
Fragments arranged by size:
1137 32 24 22
KasI G'GCGC_C
Cuts at: 0 67 1215
Size: 67 1148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 1215
Size: 86 1129
MscI TGG'CCA
Cuts at: 0 728 1215
Size: 728 487
MslI CAynn'nnrTG
Cuts at: 0 103 481 604 658 1215
Size: 103 378 123 54 557
Fragments arranged by size:
557 378 123 103 54
MspA1I CmG'CkG
Cuts at: 0 418 645 763 781 1071 1215
Size: 418 227 118 18 290 144
Fragments arranged by size:
418 290 227 144 118 18
NarI GG'CG_CC
Cuts at: 0 68 1215
Size: 68 1147
NcoI C'CATG_G
Cuts at: 0 671 1215
Size: 671 544
NheI G'CTAG_C
Cuts at: 0 1098 1215
Size: 1098 117
NspI r_CATG'y
Cuts at: 0 657 1116 1215
Size: 657 459 99
Fragments arranged by size:
657 459 99
PciI A'CATG_T
Cuts at: 0 653 1215
Size: 653 562
PflMI CCAn_nnn'nTGG
Cuts at: 0 677 1215
Size: 677 538
PfoI T'CCnGG_A
Cuts at: 0 233 248 1049 1215
Size: 233 15 801 166
Fragments arranged by size:
801 233 166 15
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 886 918 1215
Size: 886 32 297
Fragments arranged by size:
886 297 32
PstI C_TGCA'G
Cuts at: 0 1181 1215
Size: 1181 34
PvuII CAG'CTG
Cuts at: 0 645 763 1071 1215
Size: 645 118 308 144
Fragments arranged by size:
645 308 144 118
SacII CC_GC'GG
Cuts at: 0 419 782 1215
Size: 419 363 433
Fragments arranged by size:
433 419 363
SfcI C'TryA_G
Cuts at: 0 1177 1215
Size: 1177 38
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 751 1215
Size: 751 464
SfoI GGC'GCC
Cuts at: 0 69 1215
Size: 69 1146
SmlI C'TyrA_G
Cuts at: 0 115 130 1215
Size: 115 15 1085
Fragments arranged by size:
1085 115 15
StyI C'CwwG_G
Cuts at: 0 632 671 1215
Size: 632 39 544
Fragments arranged by size:
632 544 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1153 1215
Size: 1153 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 705 737 1215
Size: 705 32 478
Fragments arranged by size:
705 478 32
XbaI T'CTAG_A
Cuts at: 0 1186 1215
Size: 1186 29
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 231 1215
Size: 231 984
XmnI GAAnn'nnTTC
Cuts at: 0 22 1215
Size: 22 1193
Enzymes that do cut:
AcuI AfeI AflIII AleI AlfI AloI AlwNI AseI
AvaI BaeI BanI BbeI BbsI BcgI BglI BglII
BmrI BmtI BplI BpmI BpuEI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrDI BstYI Bsu36I BtgI DraIII DrdI EaeI EarI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI MscI
MslI MspA1I NarI NcoI NheI NspI PciI PflMI
PfoI PpiI PstI PvuII SacII SfcI SfiI SfoI
SmlI StyI TaqII TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
ApaI ApaLI ApoI AscI AsiSI AvrII BamHI BanII
BbvCI BciVI BclI BfrBI BlpI Bme1580I BmgBI Bpu10I
BsaI BsaAI BsaBI BsiEI BsiWI BsmBI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I BtgZI BtsI ClaI CspCI DraI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV
NotI NruI NsiI PacI PasI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XhoI XmaI ZraI