Restriction map for
pFN21AE6334
(Linear) (Six-base) MAPSORT of: KIEE6334.seq Check: 891 from: 1 to: 948
KIEE6334.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AclI AA'CG_TT
Cuts at: 0 370 948
Size: 370 578
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 948
Size: 560 388
AflII C'TTAA_G
Cuts at: 0 729 948
Size: 729 219
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 187 948
Size: 187 761
AlwNI CAG_nnn'CTG
Cuts at: 0 12 445 538 948
Size: 12 433 93 410
Fragments arranged by size:
433 410 93 12
ApaI G_GGCC'C
Cuts at: 0 398 948
Size: 398 550
ApoI r'AATT_y
Cuts at: 0 601 708 948
Size: 601 107 240
Fragments arranged by size:
601 240 107
AvaI C'yCGr_G
Cuts at: 0 58 87 948
Size: 58 29 861
Fragments arranged by size:
861 58 29
BanII G_rGCy'C
Cuts at: 0 398 948
Size: 398 550
BbsI GAAGACnn'nnnn_
Cuts at: 0 772 948
Size: 772 176
BlpI GC'TnA_GC
Cuts at: 0 13 948
Size: 13 935
Bme1580I G_kGCm'C
Cuts at: 0 398 948
Size: 398 550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 948
Size: 665 283
BsaAI yAC'GTr
Cuts at: 0 105 948
Size: 105 843
BseYI C'CCAG_C
Cuts at: 0 17 80 397 948
Size: 17 63 317 551
Fragments arranged by size:
551 317 63 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 948
Size: 268 680
BsmI GAATG_Cn'
Cuts at: 0 730 948
Size: 730 218
Bsp1286I G_dGCh'C
Cuts at: 0 398 948
Size: 398 550
BsrDI GCAATG_nn'
Cuts at: 0 256 948
Size: 256 692
BsrGI T'GTAC_A
Cuts at: 0 480 948
Size: 480 468
BssHII G'CGCG_C
Cuts at: 0 30 948
Size: 30 918
BstEII G'GTnAC_C
Cuts at: 0 73 948
Size: 73 875
BstYI r'GATC_y
Cuts at: 0 747 948
Size: 747 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 49 948
Size: 49 899
EarI CTCTTCn'nnn_
Cuts at: 0 575 948
Size: 575 373
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 104 948
Size: 104 844
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 665 948
Size: 560 105 283
Fragments arranged by size:
560 283 105
EcoO109I rG'GnC_Cy
Cuts at: 0 394 948
Size: 394 554
EcoRI G'AATT_C
Cuts at: 0 601 948
Size: 601 347
HincII GTy'rAC
Cuts at: 0 374 948
Size: 374 574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 948
Size: 102 846
MslI CAynn'nnrTG
Cuts at: 0 169 948
Size: 169 779
MspA1I CmG'CkG
Cuts at: 0 12 26 80 274 562 948
Size: 12 14 54 194 288 386
Fragments arranged by size:
386 288 194 54 14 12
PasI CC'CwG_GG
Cuts at: 0 330 948
Size: 330 618
PfoI T'CCnGG_A
Cuts at: 0 519 642 948
Size: 519 123 306
Fragments arranged by size:
519 306 123
PsiI TTA'TAA
Cuts at: 0 893 926 948
Size: 893 33 22
Fragments arranged by size:
893 33 22
PspOMI G'GGCC_C
Cuts at: 0 394 948
Size: 394 554
PstI C_TGCA'G
Cuts at: 0 140 243 948
Size: 140 103 705
Fragments arranged by size:
705 140 103
PvuII CAG'CTG
Cuts at: 0 274 948
Size: 274 674
ScaI AGT'ACT
Cuts at: 0 462 948
Size: 462 486
SfcI C'TryA_G
Cuts at: 0 136 239 948
Size: 136 103 709
Fragments arranged by size:
709 136 103
SmaI CCC'GGG
Cuts at: 0 60 948
Size: 60 888
SmlI C'TyrA_G
Cuts at: 0 729 948
Size: 729 219
StuI AGG'CCT
Cuts at: 0 527 948
Size: 527 421
TatI w'GTAC_w
Cuts at: 0 243 460 480 948
Size: 243 217 20 468
Fragments arranged by size:
468 243 217 20
XmaI C'CCGG_G
Cuts at: 0 58 948
Size: 58 890
XmnI GAAnn'nnTTC
Cuts at: 0 863 948
Size: 863 85
Enzymes that do cut:
AclI AcuI AflII AlfI AlwNI ApaI ApoI AvaI
BanII BbsI BlpI Bme1580I BpmI BsaAI BseYI BsgI
BsmI Bsp1286I BsrDI BsrGI BssHII BstEII BstYI BtgZI
EarI EciI Eco57MI EcoO109I EcoRI HincII MmeI MslI
MspA1I PasI PfoI PsiI PspOMI PstI PvuII ScaI
SfcI SmaI SmlI StuI TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflIII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssSI
BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StyI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI ZraI