Restriction map for
pFN21AE6329
(Linear) (Six-base) MAPSORT of: KIEE6329.seq Check: 3936 from: 1 to: 1092
KIEE6329.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1092
Size: 337 755
AfeI AGC'GCT
Cuts at: 0 860 1092
Size: 860 232
AflIII A'CryG_T
Cuts at: 0 395 1092
Size: 395 697
AhdI GACnn_n'nnGTC
Cuts at: 0 134 1092
Size: 134 958
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 487 519 1092
Size: 487 32 573
Fragments arranged by size:
573 487 32
AlwNI CAG_nnn'CTG
Cuts at: 0 301 432 1035 1092
Size: 301 131 603 57
Fragments arranged by size:
603 301 131 57
ApaI G_GGCC'C
Cuts at: 0 40 1092
Size: 40 1052
BanI G'GyrC_C
Cuts at: 0 311 622 1092
Size: 311 311 470
Fragments arranged by size:
470 311 311
BanII G_rGCy'C
Cuts at: 0 40 77 174 239 614 743 1092
Size: 40 37 97 65 375 129 349
Fragments arranged by size:
375 349 129 97 65 40 37
BbeI G_GCGC'C
Cuts at: 0 315 1092
Size: 315 777
BbsI GAAGACnn'nnnn_
Cuts at: 0 240 1092
Size: 240 852
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 717 751 1092
Size: 717 34 341
Fragments arranged by size:
717 341 34
BglI GCCn_nnn'nGGC
Cuts at: 0 601 875 1092
Size: 601 274 217
Fragments arranged by size:
601 274 217
BlpI GC'TnA_GC
Cuts at: 0 178 328 1092
Size: 178 150 764
Fragments arranged by size:
764 178 150
Bme1580I G_kGCm'C
Cuts at: 0 40 1092
Size: 40 1052
BmrI ACTGGGnnnn_n'
Cuts at: 0 883 894 1092
Size: 883 11 198
Fragments arranged by size:
883 198 11
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 1092
Size: 91 1001
BsaAI yAC'GTr
Cuts at: 0 398 1092
Size: 398 694
BsaHI Gr'CG_yC
Cuts at: 0 312 1092
Size: 312 780
BsaWI w'CCGG_w
Cuts at: 0 110 632 1092
Size: 110 522 460
Fragments arranged by size:
522 460 110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 487 517 1092
Size: 487 30 575
Fragments arranged by size:
575 487 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 535 934 1092
Size: 37 498 399 158
Fragments arranged by size:
498 399 158 37
BseYI C'CCAG_C
Cuts at: 0 88 98 301 450 596 861 1092
Size: 88 10 203 149 146 265 231
Fragments arranged by size:
265 231 203 149 146 88 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 1092
Size: 420 672
BsiHKAI G_wGCw'C
Cuts at: 0 77 989 1092
Size: 77 912 103
Fragments arranged by size:
912 103 77
BsmBI CGTCTCn'nnnn_
Cuts at: 0 916 1092
Size: 916 176
Bsp1286I G_dGCh'C
Cuts at: 0 40 77 174 239 614 743 989 1092
Size: 40 37 97 65 375 129 246 103
Fragments arranged by size:
375 246 129 103 97 65 40 37
BspEI T'CCGG_A
Cuts at: 0 110 632 1092
Size: 110 522 460
Fragments arranged by size:
522 460 110
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 671 1092
Size: 671 421
BsrBI CCG'CTC
Cuts at: 0 182 189 1092
Size: 182 7 903
Fragments arranged by size:
903 182 7
BsrDI GCAATG_nn'
Cuts at: 0 363 464 1092
Size: 363 101 628
Fragments arranged by size:
628 363 101
BsrFI r'CCGG_y
Cuts at: 0 102 600 814 1092
Size: 102 498 214 278
Fragments arranged by size:
498 278 214 102
BstAPI GCAn_nnn'nTGC
Cuts at: 0 375 432 1035 1092
Size: 375 57 603 57
Fragments arranged by size:
603 375 57 57
BstYI r'GATC_y
Cuts at: 0 203 773 1092
Size: 203 570 319
Fragments arranged by size:
570 319 203
BtgI C'CryG_G
Cuts at: 0 626 693 752 1015 1092
Size: 626 67 59 263 77
Fragments arranged by size:
626 263 77 67 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 954 1092
Size: 954 138
BtsI GCAGTG_nn'
Cuts at: 0 56 554 1092
Size: 56 498 538
Fragments arranged by size:
538 498 56
ClaI AT'CG_AT
Cuts at: 0 680 1092
Size: 680 412
EaeI y'GGCC_r
Cuts at: 0 668 755 1092
Size: 668 87 337
Fragments arranged by size:
668 337 87
EarI CTCTTCn'nnn_
Cuts at: 0 55 1001 1087 1092
Size: 55 946 86 5
Fragments arranged by size:
946 86 55 5
EcoICRI GAG'CTC
Cuts at: 0 75 1092
Size: 75 1017
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 337 1092
Size: 91 246 755
Fragments arranged by size:
755 246 91
EcoO109I rG'GnC_Cy
Cuts at: 0 305 537 867 936 1011 1092
Size: 305 232 330 69 75 81
Fragments arranged by size:
330 305 232 81 75 69
HaeII r_GCGC'y
Cuts at: 0 315 407 862 881 1023 1092
Size: 315 92 455 19 142 69
Fragments arranged by size:
455 315 142 92 69 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 120 152 1092
Size: 120 32 940
Fragments arranged by size:
940 120 32
KasI G'GCGC_C
Cuts at: 0 311 1092
Size: 311 781
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 1092
Size: 473 619
MscI TGG'CCA
Cuts at: 0 670 757 1092
Size: 670 87 335
Fragments arranged by size:
670 335 87
MspA1I CmG'CkG
Cuts at: 0 324 450 650 1035 1092
Size: 324 126 200 385 57
Fragments arranged by size:
385 324 200 126 57
NaeI GCC'GGC
Cuts at: 0 104 602 816 1092
Size: 104 498 214 276
Fragments arranged by size:
498 276 214 104
NarI GG'CG_CC
Cuts at: 0 312 1092
Size: 312 780
NcoI C'CATG_G
Cuts at: 0 752 1092
Size: 752 340
NgoMIV G'CCGG_C
Cuts at: 0 102 600 814 1092
Size: 102 498 214 278
Fragments arranged by size:
498 278 214 102
PasI CC'CwG_GG
Cuts at: 0 339 1092
Size: 339 753
PflMI CCAn_nnn'nTGG
Cuts at: 0 591 699 786 1092
Size: 591 108 87 306
Fragments arranged by size:
591 306 108 87
PfoI T'CCnGG_A
Cuts at: 0 506 1092
Size: 506 586
PmlI CAC'GTG
Cuts at: 0 398 1092
Size: 398 694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 487 519 1092
Size: 487 32 573
Fragments arranged by size:
573 487 32
PpuMI rG'GwC_Cy
Cuts at: 0 936 1011 1092
Size: 936 75 81
Fragments arranged by size:
936 81 75
PspOMI G'GGCC_C
Cuts at: 0 36 1092
Size: 36 1056
PstI C_TGCA'G
Cuts at: 0 437 609 1031 1092
Size: 437 172 422 61
Fragments arranged by size:
437 422 172 61
PvuII CAG'CTG
Cuts at: 0 324 450 1035 1092
Size: 324 126 585 57
Fragments arranged by size:
585 324 126 57
SacI G_AGCT'C
Cuts at: 0 77 1092
Size: 77 1015
SapI GCTCTTCn'nnn_
Cuts at: 0 1087 1092
Size: 1087 5
SfcI C'TryA_G
Cuts at: 0 433 605 1027 1092
Size: 433 172 422 65
Fragments arranged by size:
433 422 172 65
SfoI GGC'GCC
Cuts at: 0 313 1092
Size: 313 779
StyI C'CwwG_G
Cuts at: 0 40 752 908 974 1092
Size: 40 712 156 66 118
Fragments arranged by size:
712 156 118 66 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 706 839 1092
Size: 706 133 253
Fragments arranged by size:
706 253 133
XmnI GAAnn'nnTTC
Cuts at: 0 502 1092
Size: 502 590
Enzymes that do cut:
AcuI AfeI AflIII AhdI AloI AlwNI ApaI BanI
BanII BbeI BbsI BcgI BglI BlpI Bme1580I BmrI
BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BstAPI BstYI BtgI BtgZI BtsI ClaI EaeI EarI
EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MscI
MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SacI SapI
SfcI SfoI StyI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AleI
AlfI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbvCI BciVI BclI BfrBI BglII BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaBI BsiEI BsiWI
BsmI BspHI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MslI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI