(Linear) (Six-base) MAPSORT of: KIEE6328.seq  Check: 5155  from: 1  to: 1320



KIEE6328.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:29  ..

AccI GT'mk_AC

Cuts at:      0     139    1320 
   Size:        139    1181
 
AclI AA'CG_TT

Cuts at:      0     937    1320 
   Size:        937     383
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     736    1320 
   Size:        736     584
 
AflIII A'CryG_T

Cuts at:      0     308     794    1320 
   Size:        308     486     526

  Fragments arranged by size: 

                 526     486     308
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     744     776    1320 
   Size:        744      32     544

  Fragments arranged by size: 

                 744     544      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1048    1320 
   Size:       1048     272
 
ApoI r'AATT_y

Cuts at:      0     542    1320 
   Size:        542     778
 
AseI AT'TA_AT

Cuts at:      0     559    1320 
   Size:        559     761
 
AvaI C'yCGr_G

Cuts at:      0     442    1320 
   Size:        442     878
 
BanI G'GyrC_C

Cuts at:      0     943    1320 
   Size:        943     377
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     809    1320 
   Size:        809     511
 
BfrBI ATG'CAT

Cuts at:      0      71    1320 
   Size:         71    1249
 
Bme1580I G_kGCm'C

Cuts at:      0     998    1320 
   Size:        998     322
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     807    1320 
   Size:        807     513
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     302    1320 
   Size:        302    1018
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     481     511    1320 
   Size:        481      30     809

  Fragments arranged by size: 

                 809     481      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     577    1320 
   Size:        577     743
 
Bsp1286I G_dGCh'C

Cuts at:      0     998    1320 
   Size:        998     322
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     599    1320 
   Size:        599     721
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1048    1320 
   Size:       1048     272
 
DraI TTT'AAA

Cuts at:      0     179     782    1320 
   Size:        179     603     538

  Fragments arranged by size: 

                 603     538     179
 
EarI CTCTTCn'nnn_

Cuts at:      0    1208    1320 
   Size:       1208     112
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     736    1320 
   Size:        736     584
 
EcoO109I rG'GnC_Cy

Cuts at:      0     242    1320 
   Size:        242    1078
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     738     770    1320 
   Size:        738      32     550

  Fragments arranged by size: 

                 738     550      32
 
HincII GTy'rAC

Cuts at:      0     221    1320 
   Size:        221    1099
 
HindIII A'AGCT_T

Cuts at:      0     825    1320 
   Size:        825     495
 
HpaI GTT'AAC

Cuts at:      0     221    1320 
   Size:        221    1099
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     508    1320 
   Size:        508     812
 
MslI CAynn'nnrTG

Cuts at:      0     110    1320 
   Size:        110    1210
 
MspA1I CmG'CkG

Cuts at:      0    1048    1320 
   Size:       1048     272
 
NsiI A_TGCA'T

Cuts at:      0      73    1320 
   Size:         73    1247
 
NspI r_CATG'y

Cuts at:      0     115     312     402     495     798    1158    1320 
   Size:        115     197      90      93     303     360     162

  Fragments arranged by size: 

                 360     303     197     162     115      93      90
 
PciI A'CATG_T

Cuts at:      0     308     794    1320 
   Size:        308     486     526

  Fragments arranged by size: 

                 526     486     308
 
PpuMI rG'GwC_Cy

Cuts at:      0     242    1320 
   Size:        242    1078
 
PsiI TTA'TAA

Cuts at:      0     191    1320 
   Size:        191    1129
 
PvuII CAG'CTG

Cuts at:      0    1048    1320 
   Size:       1048     272
 
SexAI A'CCwGG_T

Cuts at:      0      57    1320 
   Size:         57    1263
 
SmlI C'TyrA_G

Cuts at:      0     442     822    1320 
   Size:        442     380     498

  Fragments arranged by size: 

                 498     442     380
 
SpeI A'CTAG_T

Cuts at:      0    1147    1320 
   Size:       1147     173
 
SphI G_CATG'C

Cuts at:      0    1158    1320 
   Size:       1158     162
 
SspI AAT'ATT

Cuts at:      0     168    1320 
   Size:        168    1152
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     284    1143    1320 
   Size:        284     859     177

  Fragments arranged by size: 

                 859     284     177
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     600     632    1320 
   Size:        600      32     688

  Fragments arranged by size: 

                 688     600      32
 
XhoI C'TCGA_G

Cuts at:      0     442    1320 
   Size:        442     878
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     AloI    AlwNI     ApoI     AseI
     AvaI     BanI    BciVI    BfrBI Bme1580I    BpuEI     BsaI    BsaXI
     BsgI Bsp1286I    BspMI   BstAPI     DraI     EarI  Eco57MI EcoO109I
    Hin4I   HincII  HindIII     HpaI     MmeI     MslI   MspA1I     NsiI
     NspI     PciI    PpuMI     PsiI    PvuII    SexAI     SmlI     SpeI
     SphI     SspI    TaqII     TstI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SrfI     StuI     StyI     SwaI
     TatI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI