Restriction map for
pFN21AE6327
(Linear) (Six-base) MAPSORT of: KIEE6327.seq Check: 3418 from: 1 to: 942
KIEE6327.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 577 942
Size: 577 365
Acc65I G'GTAC_C
Cuts at: 0 9 942
Size: 9 933
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 942
Size: 784 158
AleI CACnn'nnGTG
Cuts at: 0 145 942
Size: 145 797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 163 427 942
Size: 163 264 515
Fragments arranged by size:
515 264 163
AlwNI CAG_nnn'CTG
Cuts at: 0 370 942
Size: 370 572
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1 34 942
Size: 1 33 908
Fragments arranged by size:
908 33 1
BanI G'GyrC_C
Cuts at: 0 9 147 310 942
Size: 9 138 163 632
Fragments arranged by size:
632 163 138 9
BanII G_rGCy'C
Cuts at: 0 302 629 663 719 942
Size: 302 327 34 56 223
Fragments arranged by size:
327 302 223 56 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 862 899 942
Size: 862 37 43
Fragments arranged by size:
862 43 37
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 787 821 942
Size: 787 34 121
Fragments arranged by size:
787 121 34
BclI T'GATC_A
Cuts at: 0 599 942
Size: 599 343
BglII A'GATC_T
Cuts at: 0 458 800 942
Size: 458 342 142
Fragments arranged by size:
458 342 142
BlpI GC'TnA_GC
Cuts at: 0 809 942
Size: 809 133
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 492 942
Size: 492 450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 87 717 794 942
Size: 42 45 630 77 148
Fragments arranged by size:
630 148 77 45 42
Bpu10I CC'TnA_GC
Cuts at: 0 22 942
Size: 22 920
BsaAI yAC'GTr
Cuts at: 0 741 942
Size: 741 201
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 942
Size: 55 887
BseYI C'CCAG_C
Cuts at: 0 301 400 430 813 942
Size: 301 99 30 383 129
Fragments arranged by size:
383 301 129 99 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 942
Size: 611 331
BsiEI CG_ry'CG
Cuts at: 0 32 263 942
Size: 32 231 679
Fragments arranged by size:
679 231 32
BsiHKAI G_wGCw'C
Cuts at: 0 719 942
Size: 719 223
BsmBI CGTCTCn'nnnn_
Cuts at: 0 76 942
Size: 76 866
Bsp1286I G_dGCh'C
Cuts at: 0 302 629 663 719 942
Size: 302 327 34 56 223
Fragments arranged by size:
327 302 223 56 34
BspHI T'CATG_A
Cuts at: 0 719 942
Size: 719 223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 577 942
Size: 577 365
BsrDI GCAATG_nn'
Cuts at: 0 312 374 942
Size: 312 62 568
Fragments arranged by size:
568 312 62
BsrFI r'CCGG_y
Cuts at: 0 150 942
Size: 150 792
BstAPI GCAn_nnn'nTGC
Cuts at: 0 135 208 370 942
Size: 135 73 162 572
Fragments arranged by size:
572 162 135 73
BstEII G'GTnAC_C
Cuts at: 0 153 228 942
Size: 153 75 714
Fragments arranged by size:
714 153 75
BstXI CCAn_nnnn'nTGG
Cuts at: 0 225 942
Size: 225 717
BstYI r'GATC_y
Cuts at: 0 458 800 942
Size: 458 342 142
Fragments arranged by size:
458 342 142
BtgI C'CryG_G
Cuts at: 0 143 246 860 942
Size: 143 103 614 82
Fragments arranged by size:
614 143 103 82
BtsI GCAGTG_nn'
Cuts at: 0 411 893 942
Size: 411 482 49
Fragments arranged by size:
482 411 49
EaeI y'GGCC_r
Cuts at: 0 29 92 942
Size: 29 63 850
Fragments arranged by size:
850 63 29
EagI C'GGCC_G
Cuts at: 0 29 942
Size: 29 913
EcoICRI GAG'CTC
Cuts at: 0 717 942
Size: 717 225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 87 717 784 794 942
Size: 42 45 630 67 10 148
Fragments arranged by size:
630 148 67 45 42 10
FspI TGC'GCA
Cuts at: 0 139 942
Size: 139 803
FspAI rTGC'GCAy
Cuts at: 0 139 942
Size: 139 803
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 913 942
Size: 913 29
KpnI G_GTAC'C
Cuts at: 0 13 942
Size: 13 929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 657 942
Size: 657 285
MscI TGG'CCA
Cuts at: 0 94 942
Size: 94 848
MslI CAynn'nnrTG
Cuts at: 0 145 668 942
Size: 145 523 274
Fragments arranged by size:
523 274 145
MspA1I CmG'CkG
Cuts at: 0 107 248 361 942
Size: 107 141 113 581
Fragments arranged by size:
581 141 113 107
PfoI T'CCnGG_A
Cuts at: 0 572 942
Size: 572 370
PstI C_TGCA'G
Cuts at: 0 204 366 942
Size: 204 162 576
Fragments arranged by size:
576 204 162
PvuII CAG'CTG
Cuts at: 0 361 942
Size: 361 581
SacI G_AGCT'C
Cuts at: 0 719 942
Size: 719 223
SacII CC_GC'GG
Cuts at: 0 249 942
Size: 249 693
SfcI C'TryA_G
Cuts at: 0 192 200 362 444 942
Size: 192 8 162 82 498
Fragments arranged by size:
498 192 162 82 8
StuI AGG'CCT
Cuts at: 0 442 942
Size: 442 500
StyI C'CwwG_G
Cuts at: 0 423 942
Size: 423 519
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 249 558 636 942
Size: 249 309 78 306
Fragments arranged by size:
309 306 249 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 872 904 942
Size: 872 32 38
Fragments arranged by size:
872 38 32
XbaI T'CTAG_A
Cuts at: 0 78 942
Size: 78 864
Enzymes that do cut:
AarI Acc65I AcuI AleI AlfI AlwNI BaeI BanI
BanII BbsI BcgI BclI BglII BlpI BplI BpmI
Bpu10I BsaAI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstEII BstXI
BstYI BtgI BtsI EaeI EagI EcoICRI Eco57MI FspI
FspAI Hin4I KpnI MmeI MscI MslI MspA1I PfoI
PstI PvuII SacI SacII SfcI StuI StyI TaqII
TstI XbaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BamHI BbeI BbvCI BciVI BfrBI BglI Bme1580I
BmgBI BmrI BmtI BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BsiWI BsmI BspEI BsrBI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EarI EciI EcoNI EcoO109I EcoRI EcoRV FalI
FseI HaeII HincII HindIII HpaI KasI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TatI Tth111I XcmI XhoI XmaI
XmnI ZraI