(Linear) (Six-base) MAPSORT of: KIEE6325.seq  Check: 9077  from: 1  to: 1305



KIEE6325.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:29  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     407    1033    1305 
   Size:        310      97     626     272

  Fragments arranged by size: 

                 626     310     272      97
 
AlwNI CAG_nnn'CTG

Cuts at:      0     768    1305 
   Size:        768     537
 
ApoI r'AATT_y

Cuts at:      0     992    1087    1305 
   Size:        992      95     218

  Fragments arranged by size: 

                 992     218      95
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1224    1257    1305 
   Size:       1224      33      48

  Fragments arranged by size: 

                1224      48      33
 
BanI G'GyrC_C

Cuts at:      0    1121    1305 
   Size:       1121     184
 
BanII G_rGCy'C

Cuts at:      0     428    1305 
   Size:        428     877
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     454    1305 
   Size:        454     851
 
BclI T'GATC_A

Cuts at:      0     566    1305 
   Size:        566     739
 
BfrBI ATG'CAT

Cuts at:      0     700    1305 
   Size:        700     605
 
BglII A'GATC_T

Cuts at:      0      46    1305 
   Size:         46    1259
 
BlpI GC'TnA_GC

Cuts at:      0     427    1305 
   Size:        427     878
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     215     707    1157    1305 
   Size:        215     492     450     148

  Fragments arranged by size: 

                 492     450     215     148
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     790    1305 
   Size:        790     515
 
Bpu10I CC'TnA_GC

Cuts at:      0     778    1305 
   Size:        778     527
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190    1305 
   Size:        190    1115
 
BsaWI w'CCGG_w

Cuts at:      0     494    1305 
   Size:        494     811
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1274    1304    1305 
   Size:       1274      30       1

  Fragments arranged by size: 

                1274      30       1
 
BseYI C'CCAG_C

Cuts at:      0     432    1305 
   Size:        432     873
 
BsiHKAI G_wGCw'C

Cuts at:      0     428    1305 
   Size:        428     877
 
Bsp1286I G_dGCh'C

Cuts at:      0     428    1305 
   Size:        428     877
 
BspEI T'CCGG_A

Cuts at:      0     494    1305 
   Size:        494     811
 
BspHI T'CATG_A

Cuts at:      0     531    1305 
   Size:        531     774
 
BsrDI GCAATG_nn'

Cuts at:      0    1042    1176    1305 
   Size:       1042     134     129

  Fragments arranged by size: 

                1042     134     129
 
BstYI r'GATC_y

Cuts at:      0      46     621    1305 
   Size:         46     575     684

  Fragments arranged by size: 

                 684     575      46
 
EaeI y'GGCC_r

Cuts at:      0    1079    1305 
   Size:       1079     226
 
EarI CTCTTCn'nnn_

Cuts at:      0      68     101    1305 
   Size:         68      33    1204

  Fragments arranged by size: 

                1204      68      33
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     393    1305 
   Size:        393     912
 
EcoICRI GAG'CTC

Cuts at:      0     426    1305 
   Size:        426     879
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     407     790    1033    1305 
   Size:        310      97     383     243     272

  Fragments arranged by size: 

                 383     310     272     243      97
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1081    1305 
   Size:       1081     224
 
EcoO109I rG'GnC_Cy

Cuts at:      0     774    1305 
   Size:        774     531
 
EcoRI G'AATT_C

Cuts at:      0    1087    1305 
   Size:       1087     218
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     130    1305 
   Size:        130    1175
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     832     864    1213    1245    1305 
   Size:        832      32     349      32      60

  Fragments arranged by size: 

                 832     349      60      32      32
 
HincII GTy'rAC

Cuts at:      0    1301    1305 
   Size:       1301       4
 
HindIII A'AGCT_T

Cuts at:      0     112     292    1305 
   Size:        112     180    1013

  Fragments arranged by size: 

                1013     180     112
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      76    1305 
   Size:         76    1229
 
MscI TGG'CCA

Cuts at:      0    1081    1305 
   Size:       1081     224
 
MslI CAynn'nnrTG

Cuts at:      0    1007    1305 
   Size:       1007     298
 
MspA1I CmG'CkG

Cuts at:      0     385     804    1305 
   Size:        385     419     501

  Fragments arranged by size: 

                 501     419     385
 
NsiI A_TGCA'T

Cuts at:      0     702    1305 
   Size:        702     603
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     768    1305 
   Size:        768     537
 
PpuMI rG'GwC_Cy

Cuts at:      0     774    1305 
   Size:        774     531
 
PvuII CAG'CTG

Cuts at:      0     385     804    1305 
   Size:        385     419     501

  Fragments arranged by size: 

                 501     419     385
 
SacI G_AGCT'C

Cuts at:      0     428    1305 
   Size:        428     877
 
SmlI C'TyrA_G

Cuts at:      0     169    1305 
   Size:        169    1136
 
SspI AAT'ATT

Cuts at:      0     823    1305 
   Size:        823     482
 
StyI C'CwwG_G

Cuts at:      0    1082    1305 
   Size:       1082     223
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1109    1305 
   Size:       1109     196
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     207     239    1274    1305 
   Size:        207      32    1035      31

  Fragments arranged by size: 

                1035     207      32      31
 
XbaI T'CTAG_A

Cuts at:      0     738    1305 
   Size:        738     567
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     371    1305 
   Size:        371     934
 
XmnI GAAnn'nnTTC

Cuts at:      0     263     490    1305 
   Size:        263     227     815

  Fragments arranged by size: 

                 815     263     227
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BaeI     BanI    BanII     BbsI     BclI
    BfrBI    BglII     BlpI     BmrI     BpmI   Bpu10I    BpuEI    BsaWI
    BsaXI    BseYI  BsiHKAI Bsp1286I    BspEI    BspHI    BsrDI    BstYI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI    Hin4I   HincII  HindIII     MmeI     MscI     MslI   MspA1I
     NsiI    PflMI    PpuMI    PvuII     SacI     SmlI     SspI     StyI
    TaqII     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI     BbeI    BbvCI     BcgI
    BciVI     BglI Bme1580I    BmgBI     BmtI     BplI     BsaI    BsaAI
    BsaBI    BsaHI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI    EcoRV     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI     TatI  Tth111I     XhoI     XmaI     ZraI