Restriction map for
pFN21AE6324
(Linear) (Six-base) MAPSORT of: KIEE6324.seq Check: 4267 from: 1 to: 1314
KIEE6324.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 911 1154 1314
Size: 911 243 160
Fragments arranged by size:
911 243 160
Acc65I G'GTAC_C
Cuts at: 0 250 444 1314
Size: 250 194 870
Fragments arranged by size:
870 250 194
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1038 1314
Size: 706 332 276
Fragments arranged by size:
706 332 276
AfeI AGC'GCT
Cuts at: 0 577 1314
Size: 577 737
AlwNI CAG_nnn'CTG
Cuts at: 0 616 804 1314
Size: 616 188 510
Fragments arranged by size:
616 510 188
ApaI G_GGCC'C
Cuts at: 0 693 1270 1314
Size: 693 577 44
Fragments arranged by size:
693 577 44
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 280 313 1314
Size: 280 33 1001
Fragments arranged by size:
1001 280 33
BamHI G'GATC_C
Cuts at: 0 963 1314
Size: 963 351
BanI G'GyrC_C
Cuts at: 0 95 250 444 1314
Size: 95 155 194 870
Fragments arranged by size:
870 194 155 95
BanII G_rGCy'C
Cuts at: 0 693 1172 1181 1270 1314
Size: 693 479 9 89 44
Fragments arranged by size:
693 479 89 44 9
BbeI G_GCGC'C
Cuts at: 0 99 1314
Size: 99 1215
BbsI GAAGACnn'nnnn_
Cuts at: 0 408 1314
Size: 408 906
BbvCI CC'TCA_GC
Cuts at: 0 958 1314
Size: 958 356
BglI GCCn_nnn'nGGC
Cuts at: 0 223 1314
Size: 223 1091
Bme1580I G_kGCm'C
Cuts at: 0 693 1270 1314
Size: 693 577 44
Fragments arranged by size:
693 577 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 520 1314
Size: 520 794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 1314
Size: 772 542
Bpu10I CC'TnA_GC
Cuts at: 0 958 1150 1314
Size: 958 192 164
Fragments arranged by size:
958 192 164
BsaHI Gr'CG_yC
Cuts at: 0 96 239 1314
Size: 96 143 1075
Fragments arranged by size:
1075 143 96
BsaWI w'CCGG_w
Cuts at: 0 31 283 1314
Size: 31 252 1031
Fragments arranged by size:
1031 252 31
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 655 1314
Size: 655 659
BseYI C'CCAG_C
Cuts at: 0 7 300 572 578 584 702 732 760
Size: 7 293 272 6 6 118 30 28
Cuts at: 760 989 1164 1263 1314
Size: 229 175 99 51
Fragments arranged by size:
293 272 229 175 118 99 51 30
28 7 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1112 1210 1314
Size: 1112 98 104
Fragments arranged by size:
1112 104 98
BsiEI CG_ry'CG
Cuts at: 0 27 1314
Size: 27 1287
BsiHKAI G_wGCw'C
Cuts at: 0 392 1181 1291 1314
Size: 392 789 110 23
Fragments arranged by size:
789 392 110 23
Bsp1286I G_dGCh'C
Cuts at: 0 392 693 1172 1181 1270 1291 1314
Size: 392 301 479 9 89 21 23
Fragments arranged by size:
479 392 301 89 23 21 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 445 911 1154 1314
Size: 445 466 243 160
Fragments arranged by size:
466 445 243 160
BsrBI CCG'CTC
Cuts at: 0 93 779 1061 1314
Size: 93 686 282 253
Fragments arranged by size:
686 282 253 93
BsrDI GCAATG_nn'
Cuts at: 0 73 1314
Size: 73 1241
BsrFI r'CCGG_y
Cuts at: 0 26 1314
Size: 26 1288
BsrGI T'GTAC_A
Cuts at: 0 314 1314
Size: 314 1000
BssHII G'CGCG_C
Cuts at: 0 78 1314
Size: 78 1236
BstAPI GCAn_nnn'nTGC
Cuts at: 0 72 1314
Size: 72 1242
BstYI r'GATC_y
Cuts at: 0 963 1314
Size: 963 351
BtgI C'CryG_G
Cuts at: 0 38 533 684 1314
Size: 38 495 151 630
Fragments arranged by size:
630 495 151 38
BtsI GCAGTG_nn'
Cuts at: 0 1099 1314
Size: 1099 215
DraIII CAC_nnn'GTG
Cuts at: 0 189 1314
Size: 189 1125
EaeI y'GGCC_r
Cuts at: 0 24 134 224 530 792 1314
Size: 24 110 90 306 262 522
Fragments arranged by size:
522 306 262 110 90 24
EagI C'GGCC_G
Cuts at: 0 24 1314
Size: 24 1290
EarI CTCTTCn'nnn_
Cuts at: 0 520 550 643 853 1030 1314
Size: 520 30 93 210 177 284
Fragments arranged by size:
520 284 210 177 93 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 169 1314
Size: 169 1145
EcoICRI GAG'CTC
Cuts at: 0 1179 1314
Size: 1179 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 772 1038 1314
Size: 706 66 266 276
Fragments arranged by size:
706 276 266 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 154 838 972 1314
Size: 154 684 134 342
Fragments arranged by size:
684 342 154 134
EcoO109I rG'GnC_Cy
Cuts at: 0 689 690 1314
Size: 689 1 624
Fragments arranged by size:
689 624 1
HaeII r_GCGC'y
Cuts at: 0 99 579 831 847 1314
Size: 99 480 252 16 467
Fragments arranged by size:
480 467 252 99 16
HincII GTy'rAC
Cuts at: 0 881 1314
Size: 881 433
KasI G'GCGC_C
Cuts at: 0 95 1314
Size: 95 1219
KpnI G_GTAC'C
Cuts at: 0 254 448 1314
Size: 254 194 866
Fragments arranged by size:
866 254 194
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1058 1314
Size: 1058 256
MscI TGG'CCA
Cuts at: 0 532 1314
Size: 532 782
MslI CAynn'nnrTG
Cuts at: 0 322 364 502 1314
Size: 322 42 138 812
Fragments arranged by size:
812 322 138 42
MspA1I CmG'CkG
Cuts at: 0 40 304 764 1314
Size: 40 264 460 550
Fragments arranged by size:
550 460 264 40
NarI GG'CG_CC
Cuts at: 0 96 1314
Size: 96 1218
NcoI C'CATG_G
Cuts at: 0 533 1314
Size: 533 781
NdeI CA'TA_TG
Cuts at: 0 1231 1314
Size: 1231 83
PasI CC'CwG_GG
Cuts at: 0 646 1314
Size: 646 668
PfoI T'CCnGG_A
Cuts at: 0 1183 1314
Size: 1183 131
PspOMI G'GGCC_C
Cuts at: 0 689 1266 1314
Size: 689 577 48
Fragments arranged by size:
689 577 48
PstI C_TGCA'G
Cuts at: 0 77 275 922 1213 1256 1314
Size: 77 198 647 291 43 58
Fragments arranged by size:
647 291 198 77 58 43
PvuII CAG'CTG
Cuts at: 0 304 1314
Size: 304 1010
RsrII CG'GwC_CG
Cuts at: 0 29 1314
Size: 29 1285
SacI G_AGCT'C
Cuts at: 0 1181 1314
Size: 1181 133
SacII CC_GC'GG
Cuts at: 0 41 1314
Size: 41 1273
SapI GCTCTTCn'nnn_
Cuts at: 0 520 550 643 853 1314
Size: 520 30 93 210 461
Fragments arranged by size:
520 461 210 93 30
SfcI C'TryA_G
Cuts at: 0 73 271 918 1209 1252 1314
Size: 73 198 647 291 43 62
Fragments arranged by size:
647 291 198 73 62 43
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 223 1314
Size: 223 1091
SfoI GGC'GCC
Cuts at: 0 97 1314
Size: 97 1217
StuI AGG'CCT
Cuts at: 0 217 1314
Size: 217 1097
StyI C'CwwG_G
Cuts at: 0 533 789 1314
Size: 533 256 525
Fragments arranged by size:
533 525 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1017 1306 1314
Size: 1017 289 8
Fragments arranged by size:
1017 289 8
TatI w'GTAC_w
Cuts at: 0 314 332 1314
Size: 314 18 982
Fragments arranged by size:
982 314 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 278 310 1314
Size: 278 32 1004
Fragments arranged by size:
1004 278 32
Enzymes that do cut:
AarI Acc65I AcuI AfeI AlwNI ApaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BglI Bme1580I BmrI
BpmI Bpu10I BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstYI BtgI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII HincII KasI
KpnI MmeI MscI MslI MspA1I NarI NcoI NdeI
PasI PfoI PspOMI PstI PvuII RsrII SacI SacII
SapI SfcI SfiI SfoI StuI StyI TaqII TatI
TstI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaXI BsiWI
BsmI BsmBI BspEI BspHI BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI
MfeI MluI NaeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI SalI SanDI SbfI
ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI