Restriction map for
pFN21AE6322
(Linear) (Six-base) MAPSORT of: KIEE6322.seq Check: 4669 from: 1 to: 966
KIEE6322.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AccI GT'mk_AC
Cuts at: 0 634 966
Size: 634 332
Acc65I G'GTAC_C
Cuts at: 0 704 966
Size: 704 262
BamHI G'GATC_C
Cuts at: 0 86 966
Size: 86 880
BanI G'GyrC_C
Cuts at: 0 21 286 704 966
Size: 21 265 418 262
Fragments arranged by size:
418 265 262 21
BanII G_rGCy'C
Cuts at: 0 585 677 966
Size: 585 92 289
Fragments arranged by size:
585 289 92
BbsI GAAGACnn'nnnn_
Cuts at: 0 159 961 966
Size: 159 802 5
Fragments arranged by size:
802 159 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 624 658 966
Size: 624 34 308
Fragments arranged by size:
624 308 34
BclI T'GATC_A
Cuts at: 0 182 966
Size: 182 784
BglI GCCn_nnn'nGGC
Cuts at: 0 30 966
Size: 30 936
BglII A'GATC_T
Cuts at: 0 203 966
Size: 203 763
Bme1580I G_kGCm'C
Cuts at: 0 26 753 898 966
Size: 26 727 145 68
Fragments arranged by size:
727 145 68 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 361 677 966
Size: 361 316 289
Fragments arranged by size:
361 316 289
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 538 966
Size: 538 428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 467 966
Size: 282 185 499
Fragments arranged by size:
499 282 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 966
Size: 708 258
BsaI GGTCTCn'nnnn_
Cuts at: 0 300 966
Size: 300 666
BsaBI GATnn'nnATC
Cuts at: 0 208 966
Size: 208 758
BseYI C'CCAG_C
Cuts at: 0 645 966
Size: 645 321
BsiEI CG_ry'CG
Cuts at: 0 30 966
Size: 30 936
BsiHKAI G_wGCw'C
Cuts at: 0 677 966
Size: 677 289
BsmBI CGTCTCn'nnnn_
Cuts at: 0 133 966
Size: 133 833
Bsp1286I G_dGCh'C
Cuts at: 0 26 585 677 753 898 966
Size: 26 559 92 76 145 68
Fragments arranged by size:
559 145 92 76 68 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 811 966
Size: 811 155
BsrBI CCG'CTC
Cuts at: 0 45 966
Size: 45 921
BsrDI GCAATG_nn'
Cuts at: 0 637 966
Size: 637 329
BsrFI r'CCGG_y
Cuts at: 0 29 966
Size: 29 937
BsrGI T'GTAC_A
Cuts at: 0 464 966
Size: 464 502
BstEII G'GTnAC_C
Cuts at: 0 384 966
Size: 384 582
BstXI CCAn_nnnn'nTGG
Cuts at: 0 753 966
Size: 753 213
BstYI r'GATC_y
Cuts at: 0 86 203 966
Size: 86 117 763
Fragments arranged by size:
763 117 86
BtgI C'CryG_G
Cuts at: 0 290 966
Size: 290 676
BtsI GCAGTG_nn'
Cuts at: 0 224 892 966
Size: 224 668 74
Fragments arranged by size:
668 224 74
EaeI y'GGCC_r
Cuts at: 0 413 966
Size: 413 553
EarI CTCTTCn'nnn_
Cuts at: 0 865 966
Size: 865 101
EcoICRI GAG'CTC
Cuts at: 0 675 966
Size: 675 291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 467 966
Size: 282 185 499
Fragments arranged by size:
499 282 185
EcoO109I rG'GnC_Cy
Cuts at: 0 339 966
Size: 339 627
HincII GTy'rAC
Cuts at: 0 324 635 966
Size: 324 311 331
Fragments arranged by size:
331 324 311
KpnI G_GTAC'C
Cuts at: 0 708 966
Size: 708 258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 966
Size: 626 340
MscI TGG'CCA
Cuts at: 0 415 966
Size: 415 551
MslI CAynn'nnrTG
Cuts at: 0 772 966
Size: 772 194
NcoI C'CATG_G
Cuts at: 0 290 966
Size: 290 676
NspI r_CATG'y
Cuts at: 0 579 966
Size: 579 387
PasI CC'CwG_GG
Cuts at: 0 753 966
Size: 753 213
PflMI CCAn_nnn'nTGG
Cuts at: 0 887 966
Size: 887 79
PpuMI rG'GwC_Cy
Cuts at: 0 339 966
Size: 339 627
PstI C_TGCA'G
Cuts at: 0 95 966
Size: 95 871
SacI G_AGCT'C
Cuts at: 0 677 966
Size: 677 289
SalI G'TCGA_C
Cuts at: 0 633 966
Size: 633 333
SbfI CC_TGCA'GG
Cuts at: 0 95 966
Size: 95 871
SfcI C'TryA_G
Cuts at: 0 91 313 966
Size: 91 222 653
Fragments arranged by size:
653 222 91
SmlI C'TyrA_G
Cuts at: 0 687 966
Size: 687 279
StyI C'CwwG_G
Cuts at: 0 290 342 378 966
Size: 290 52 36 588
Fragments arranged by size:
588 290 52 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 46 274 879 966
Size: 46 228 605 87
Fragments arranged by size:
605 228 87 46
TatI w'GTAC_w
Cuts at: 0 464 966
Size: 464 502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 888 966
Size: 888 78
Enzymes that do cut:
AccI Acc65I BamHI BanI BanII BbsI BcgI BclI
BglI BglII Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaBI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrGI BstEII BstXI BstYI BtgI BtsI
EaeI EarI EcoICRI Eco57MI EcoO109I HincII KpnI MmeI
MscI MslI NcoI NspI PasI PflMI PpuMI PstI
SacI SalI SbfI SfcI SmlI StyI TaqII TatI
XcmI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI
BciVI BfrBI BlpI BmgBI BmtI Bpu10I BsaAI BsaHI
BsaWI BsaXI BseRI BsgI BsiWI BsmI BspEI BspHI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HindIII
HpaI KasI MfeI MluI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SanDI SapI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI