Restriction map for
pFN21AE6320
(Linear) (Six-base) MAPSORT of: KIEE6320.seq Check: 8461 from: 1 to: 1203
KIEE6320.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 952 1088 1203
Size: 538 414 136 115
Fragments arranged by size:
538 414 136 115
AfeI AGC'GCT
Cuts at: 0 38 361 1203
Size: 38 323 842
Fragments arranged by size:
842 323 38
AflIII A'CryG_T
Cuts at: 0 1028 1203
Size: 1028 175
AlwNI CAG_nnn'CTG
Cuts at: 0 375 1002 1203
Size: 375 627 201
Fragments arranged by size:
627 375 201
ApoI r'AATT_y
Cuts at: 0 874 1203
Size: 874 329
AscI GG'CGCG_CC
Cuts at: 0 72 1203
Size: 72 1131
AvaI C'yCGr_G
Cuts at: 0 130 280 310 1203
Size: 130 150 30 893
Fragments arranged by size:
893 150 130 30
BanI G'GyrC_C
Cuts at: 0 11 577 1203
Size: 11 566 626
Fragments arranged by size:
626 566 11
BanII G_rGCy'C
Cuts at: 0 287 440 1203
Size: 287 153 763
Fragments arranged by size:
763 287 153
BbeI G_GCGC'C
Cuts at: 0 15 1203
Size: 15 1188
BclI T'GATC_A
Cuts at: 0 972 1203
Size: 972 231
BmrI ACTGGGnnnn_n'
Cuts at: 0 186 841 1203
Size: 186 655 362
Fragments arranged by size:
655 362 186
BmtI G_CTAG'C
Cuts at: 0 37 1203
Size: 37 1166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 684 1084 1203
Size: 220 464 400 119
Fragments arranged by size:
464 400 220 119
Bpu10I CC'TnA_GC
Cuts at: 0 1124 1203
Size: 1124 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1203
Size: 340 863
BsaI GGTCTCn'nnnn_
Cuts at: 0 1189 1203
Size: 1189 14
BsaAI yAC'GTr
Cuts at: 0 1031 1203
Size: 1031 172
BsaHI Gr'CG_yC
Cuts at: 0 12 1203
Size: 12 1191
BsaWI w'CCGG_w
Cuts at: 0 864 1203
Size: 864 339
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1145 1175 1203
Size: 1145 30 28
Fragments arranged by size:
1145 30 28
BseYI C'CCAG_C
Cuts at: 0 395 400 1203
Size: 395 5 803
Fragments arranged by size:
803 395 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 971 1203
Size: 202 769 232
Fragments arranged by size:
769 232 202
BsiHKAI G_wGCw'C
Cuts at: 0 176 1052 1203
Size: 176 876 151
Fragments arranged by size:
876 176 151
BsmI GAATG_Cn'
Cuts at: 0 820 942 1203
Size: 820 122 261
Fragments arranged by size:
820 261 122
Bsp1286I G_dGCh'C
Cuts at: 0 176 287 440 1052 1203
Size: 176 111 153 612 151
Fragments arranged by size:
612 176 153 151 111
BsrBI CCG'CTC
Cuts at: 0 129 1203
Size: 129 1074
BsrFI r'CCGG_y
Cuts at: 0 143 292 1203
Size: 143 149 911
Fragments arranged by size:
911 149 143
BssHII G'CGCG_C
Cuts at: 0 56 63 72 344 1203
Size: 56 7 9 272 859
Fragments arranged by size:
859 272 56 9 7
BstAPI GCAn_nnn'nTGC
Cuts at: 0 225 375 1203
Size: 225 150 828
Fragments arranged by size:
828 225 150
BtgI C'CryG_G
Cuts at: 0 23 109 1203
Size: 23 86 1094
Fragments arranged by size:
1094 86 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 422 477 1203
Size: 422 55 726
Fragments arranged by size:
726 422 55
BtsI GCAGTG_nn'
Cuts at: 0 348 644 1203
Size: 348 296 559
Fragments arranged by size:
559 348 296
EarI CTCTTCn'nnn_
Cuts at: 0 328 538 827 1203
Size: 328 210 289 376
Fragments arranged by size:
376 328 289 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 376 433 1203
Size: 376 57 770
Fragments arranged by size:
770 376 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 538 684 952 1084 1088 1203
Size: 220 318 146 268 132 4 115
Fragments arranged by size:
318 268 220 146 132 115 4
EcoO109I rG'GnC_Cy
Cuts at: 0 330 372 1035 1203
Size: 330 42 663 168
Fragments arranged by size:
663 330 168 42
HaeII r_GCGC'y
Cuts at: 0 15 31 40 269 363 432 1203
Size: 15 16 9 229 94 69 771
Fragments arranged by size:
771 229 94 69 16 15 9
KasI G'GCGC_C
Cuts at: 0 11 1203
Size: 11 1192
MslI CAynn'nnrTG
Cuts at: 0 527 682 1203
Size: 527 155 521
Fragments arranged by size:
527 521 155
MspA1I CmG'CkG
Cuts at: 0 25 167 499 1203
Size: 25 142 332 704
Fragments arranged by size:
704 332 142 25
NaeI GCC'GGC
Cuts at: 0 294 1203
Size: 294 909
NarI GG'CG_CC
Cuts at: 0 12 1203
Size: 12 1191
NdeI CA'TA_TG
Cuts at: 0 615 1203
Size: 615 588
NgoMIV G'CCGG_C
Cuts at: 0 292 1203
Size: 292 911
NheI G'CTAG_C
Cuts at: 0 33 1203
Size: 33 1170
PmlI CAC'GTG
Cuts at: 0 1031 1203
Size: 1031 172
PsiI TTA'TAA
Cuts at: 0 563 917 1203
Size: 563 354 286
Fragments arranged by size:
563 354 286
PspXI vC'TCGA_Gb
Cuts at: 0 130 1203
Size: 130 1073
PstI C_TGCA'G
Cuts at: 0 265 455 1203
Size: 265 190 748
Fragments arranged by size:
748 265 190
SacII CC_GC'GG
Cuts at: 0 26 1203
Size: 26 1177
SexAI A'CCwGG_T
Cuts at: 0 1001 1203
Size: 1001 202
SfcI C'TryA_G
Cuts at: 0 90 261 451 1203
Size: 90 171 190 752
Fragments arranged by size:
752 190 171 90
SfoI GGC'GCC
Cuts at: 0 13 1203
Size: 13 1190
SmlI C'TyrA_G
Cuts at: 0 130 355 1203
Size: 130 225 848
Fragments arranged by size:
848 225 130
StuI AGG'CCT
Cuts at: 0 244 1203
Size: 244 959
TatI w'GTAC_w
Cuts at: 0 161 1203
Size: 161 1042
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1145 1177 1203
Size: 1145 32 26
Fragments arranged by size:
1145 32 26
Tth111I GACn'n_nGTC
Cuts at: 0 985 1203
Size: 985 218
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 681 1203
Size: 681 522
XhoI C'TCGA_G
Cuts at: 0 130 1203
Size: 130 1073
XmnI GAAnn'nnTTC
Cuts at: 0 1073 1203
Size: 1073 130
Enzymes that do cut:
AcuI AfeI AflIII AlwNI ApoI AscI AvaI BanI
BanII BbeI BclI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiHKAI
BsmI Bsp1286I BsrBI BsrFI BssHII BstAPI BtgI BtgZI
BtsI EarI EciI Eco57MI EcoO109I HaeII KasI MslI
MspA1I NaeI NarI NdeI NgoMIV NheI PmlI PsiI
PspXI PstI SacII SexAI SfcI SfoI SmlI StuI
TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI Bme1580I BmgBI BplI BsaBI BseRI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssSI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EaeI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI NcoI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PsrI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StyI SwaI TaqII XbaI XmaI ZraI