Restriction map for
pFN21AE6319
(Linear) (Six-base) MAPSORT of: KIEE6319.seq Check: 6929 from: 1 to: 1188
KIEE6319.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 323 1188
Size: 323 865
AccI GT'mk_AC
Cuts at: 0 704 1188
Size: 704 484
AflIII A'CryG_T
Cuts at: 0 581 1188
Size: 581 607
AleI CACnn'nnGTG
Cuts at: 0 637 1188
Size: 637 551
AlwNI CAG_nnn'CTG
Cuts at: 0 97 1188
Size: 97 1091
ApaI G_GGCC'C
Cuts at: 0 116 516 1188
Size: 116 400 672
Fragments arranged by size:
672 400 116
ApoI r'AATT_y
Cuts at: 0 568 1188
Size: 568 620
AvaI C'yCGr_G
Cuts at: 0 191 281 509 1188
Size: 191 90 228 679
Fragments arranged by size:
679 228 191 90
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 160 193 1188
Size: 160 33 995
Fragments arranged by size:
995 160 33
BamHI G'GATC_C
Cuts at: 0 3 1188
Size: 3 1185
BanI G'GyrC_C
Cuts at: 0 131 398 1188
Size: 131 267 790
Fragments arranged by size:
790 267 131
BanII G_rGCy'C
Cuts at: 0 116 198 516 761 837 1188
Size: 116 82 318 245 76 351
Fragments arranged by size:
351 318 245 116 82 76
BbeI G_GCGC'C
Cuts at: 0 135 1188
Size: 135 1053
BbsI GAAGACnn'nnnn_
Cuts at: 0 40 1188
Size: 40 1148
BciVI GTATCCnnnnn_n'
Cuts at: 0 253 556 1188
Size: 253 303 632
Fragments arranged by size:
632 303 253
BlpI GC'TnA_GC
Cuts at: 0 47 1188
Size: 47 1141
Bme1580I G_kGCm'C
Cuts at: 0 116 401 516 1188
Size: 116 285 115 672
Fragments arranged by size:
672 285 116 115
BmgBI CAC'GTC
Cuts at: 0 389 1188
Size: 389 799
BmrI ACTGGGnnnn_n'
Cuts at: 0 154 1072 1188
Size: 154 918 116
Fragments arranged by size:
918 154 116
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 542 1188
Size: 542 646
Bpu10I CC'TnA_GC
Cuts at: 0 1003 1188
Size: 1003 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 892 1188
Size: 745 147 296
Fragments arranged by size:
745 296 147
BsaHI Gr'CG_yC
Cuts at: 0 132 1188
Size: 132 1056
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 524 538 554 568 803 833 1164 1188
Size: 524 14 16 14 235 30 331 24
Fragments arranged by size:
524 331 235 30 24 16 14 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 38 304 529 813 1188
Size: 38 266 225 284 375
Fragments arranged by size:
375 284 266 225 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 693 1188
Size: 608 85 495
Fragments arranged by size:
608 495 85
BsiHKAI G_wGCw'C
Cuts at: 0 761 1021 1188
Size: 761 260 167
Fragments arranged by size:
761 260 167
BsmBI CGTCTCn'nnnn_
Cuts at: 0 248 1188
Size: 248 940
Bsp1286I G_dGCh'C
Cuts at: 0 116 198 401 516 761 837 1021 1188
Size: 116 82 203 115 245 76 184 167
Fragments arranged by size:
245 203 184 167 116 115 82 76
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 323 754 1188
Size: 323 431 434
Fragments arranged by size:
434 431 323
BsrBI CCG'CTC
Cuts at: 0 432 537 1188
Size: 432 105 651
Fragments arranged by size:
651 432 105
BsrFI r'CCGG_y
Cuts at: 0 128 344 1188
Size: 128 216 844
Fragments arranged by size:
844 216 128
BsrGI T'GTAC_A
Cuts at: 0 554 1092 1188
Size: 554 538 96
Fragments arranged by size:
554 538 96
BstXI CCAn_nnnn'nTGG
Cuts at: 0 181 1188
Size: 181 1007
BstYI r'GATC_y
Cuts at: 0 3 1188
Size: 3 1185
BtgI C'CryG_G
Cuts at: 0 180 1188
Size: 180 1008
ClaI AT'CG_AT
Cuts at: 0 270 1188
Size: 270 918
DrdI GACnn_nn'nnGTC
Cuts at: 0 292 895 1188
Size: 292 603 293
Fragments arranged by size:
603 293 292
EaeI y'GGCC_r
Cuts at: 0 529 789 1188
Size: 529 260 399
Fragments arranged by size:
529 399 260
EarI CTCTTCn'nnn_
Cuts at: 0 694 906 1060 1188
Size: 694 212 154 128
Fragments arranged by size:
694 212 154 128
EcoICRI GAG'CTC
Cuts at: 0 759 1188
Size: 759 429
EcoO109I rG'GnC_Cy
Cuts at: 0 113 235 855 1152 1188
Size: 113 122 620 297 36
Fragments arranged by size:
620 297 122 113 36
HaeII r_GCGC'y
Cuts at: 0 135 1188
Size: 135 1053
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 803 835 1144 1164 1176 1188
Size: 803 32 309 20 12 12
Fragments arranged by size:
803 309 32 20 12 12
HincII GTy'rAC
Cuts at: 0 630 1188
Size: 630 558
HpaI GTT'AAC
Cuts at: 0 630 1188
Size: 630 558
KasI G'GCGC_C
Cuts at: 0 131 1188
Size: 131 1057
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1188
Size: 329 859
MscI TGG'CCA
Cuts at: 0 791 1188
Size: 791 397
MslI CAynn'nnrTG
Cuts at: 0 185 637 928 1100 1188
Size: 185 452 291 172 88
Fragments arranged by size:
452 291 185 172 88
MspA1I CmG'CkG
Cuts at: 0 450 1188
Size: 450 738
NarI GG'CG_CC
Cuts at: 0 132 1188
Size: 132 1056
NcoI C'CATG_G
Cuts at: 0 180 1188
Size: 180 1008
NdeI CA'TA_TG
Cuts at: 0 1126 1188
Size: 1126 62
NspI r_CATG'y
Cuts at: 0 585 1188
Size: 585 603
PasI CC'CwG_GG
Cuts at: 0 8 850 1188
Size: 8 842 338
Fragments arranged by size:
842 338 8
PciI A'CATG_T
Cuts at: 0 581 1188
Size: 581 607
PfoI T'CCnGG_A
Cuts at: 0 296 329 542 1028 1188
Size: 296 33 213 486 160
Fragments arranged by size:
486 296 213 160 33
PpuMI rG'GwC_Cy
Cuts at: 0 855 1152 1188
Size: 855 297 36
Fragments arranged by size:
855 297 36
PspOMI G'GGCC_C
Cuts at: 0 112 512 1188
Size: 112 400 676
Fragments arranged by size:
676 400 112
PvuII CAG'CTG
Cuts at: 0 450 1188
Size: 450 738
SacI G_AGCT'C
Cuts at: 0 761 1188
Size: 761 427
SanDI GG'GwC_CC
Cuts at: 0 855 1188
Size: 855 333
SapI GCTCTTCn'nnn_
Cuts at: 0 694 1188
Size: 694 494
SfcI C'TryA_G
Cuts at: 0 207 807 1188
Size: 207 600 381
Fragments arranged by size:
600 381 207
SfoI GGC'GCC
Cuts at: 0 133 1188
Size: 133 1055
SmaI CCC'GGG
Cuts at: 0 283 1188
Size: 283 905
SmlI C'TyrA_G
Cuts at: 0 760 907 1188
Size: 760 147 281
Fragments arranged by size:
760 281 147
StyI C'CwwG_G
Cuts at: 0 180 770 1188
Size: 180 590 418
Fragments arranged by size:
590 418 180
TatI w'GTAC_w
Cuts at: 0 554 578 1092 1188
Size: 554 24 514 96
Fragments arranged by size:
554 514 96 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 213 245 524 556 939 971 1188
Size: 213 32 279 32 383 32 217
Fragments arranged by size:
383 279 217 213 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1131 1188
Size: 1131 57
XmaI C'CCGG_G
Cuts at: 0 281 1188
Size: 281 907
Enzymes that do cut:
AarI AccI AflIII AleI AlwNI ApaI ApoI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaHI BsaXI
BseRI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI
BsrGI BstXI BstYI BtgI ClaI DrdI EaeI EarI
EcoICRI EcoO109I HaeII Hin4I HincII HpaI KasI MmeI
MscI MslI MspA1I NarI NcoI NdeI NspI PasI
PciI PfoI PpuMI PspOMI PvuII SacI SanDI SapI
SfcI SfoI SmaI SmlI StyI TatI TstI XcmI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BbvCI
BcgI BclI BfrBI BglI BglII BmtI BpmI BsaI
BsaAI BsaBI BsaWI BseYI BsiEI BsiWI BsmI BspEI
BspHI BsrDI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI CspCI DraI DraIII EagI EciI
Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII KpnI MfeI MluI NaeI NgoMIV NheI NotI
NruI NsiI PacI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI
XhoI XmnI ZraI