Restriction map for
pFN21AE6316
(Linear) (Six-base) MAPSORT of: KIEE6316.seq Check: 1709 from: 1 to: 1254
KIEE6316.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 1254
Size: 1051 203
AflIII A'CryG_T
Cuts at: 0 125 1254
Size: 125 1129
ApaLI G'TGCA_C
Cuts at: 0 29 1254
Size: 29 1225
ApoI r'AATT_y
Cuts at: 0 160 523 1254
Size: 160 363 731
Fragments arranged by size:
731 363 160
AseI AT'TA_AT
Cuts at: 0 770 850 1254
Size: 770 80 404
Fragments arranged by size:
770 404 80
BanI G'GyrC_C
Cuts at: 0 399 1254
Size: 399 855
BanII G_rGCy'C
Cuts at: 0 450 884 1254
Size: 450 434 370
Fragments arranged by size:
450 434 370
BbsI GAAGACnn'nnnn_
Cuts at: 0 596 1254
Size: 596 658
BglI GCCn_nnn'nGGC
Cuts at: 0 565 1254
Size: 565 689
Bme1580I G_kGCm'C
Cuts at: 0 33 1254
Size: 33 1221
BmrI ACTGGGnnnn_n'
Cuts at: 0 1158 1254
Size: 1158 96
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 466 490 1254
Size: 466 24 764
Fragments arranged by size:
764 466 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 1254
Size: 446 808
Bpu10I CC'TnA_GC
Cuts at: 0 678 1254
Size: 678 576
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 890 1254
Size: 122 768 364
Fragments arranged by size:
768 364 122
BsaI GGTCTCn'nnnn_
Cuts at: 0 235 245 478 1254
Size: 235 10 233 776
Fragments arranged by size:
776 235 233 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 376 406 1145 1175 1254
Size: 376 30 739 30 79
Fragments arranged by size:
739 376 79 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 458 649 1097 1169 1254
Size: 458 191 448 72 85
Fragments arranged by size:
458 448 191 85 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 1163 1254
Size: 827 336 91
Fragments arranged by size:
827 336 91
BsiHKAI G_wGCw'C
Cuts at: 0 33 363 450 884 1254
Size: 33 330 87 434 370
Fragments arranged by size:
434 370 330 87 33
BsmI GAATG_Cn'
Cuts at: 0 794 1254
Size: 794 460
Bsp1286I G_dGCh'C
Cuts at: 0 33 363 450 884 1254
Size: 33 330 87 434 370
Fragments arranged by size:
434 370 330 87 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 602 1254
Size: 602 652
BstXI CCAn_nnnn'nTGG
Cuts at: 0 867 1254
Size: 867 387
BstYI r'GATC_y
Cuts at: 0 818 1202 1254
Size: 818 384 52
Fragments arranged by size:
818 384 52
BtgI C'CryG_G
Cuts at: 0 224 758 1254
Size: 224 534 496
Fragments arranged by size:
534 496 224
DraIII CAC_nnn'GTG
Cuts at: 0 406 1254
Size: 406 848
EarI CTCTTCn'nnn_
Cuts at: 0 238 481 935 967 1027 1254
Size: 238 243 454 32 60 227
Fragments arranged by size:
454 243 238 227 60 32
EcoICRI GAG'CTC
Cuts at: 0 448 882 1254
Size: 448 434 372
Fragments arranged by size:
448 434 372
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 1051 1254
Size: 446 605 203
Fragments arranged by size:
605 446 203
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1076 1254
Size: 1076 178
EcoRI G'AATT_C
Cuts at: 0 160 1254
Size: 160 1094
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 334 454 1254
Size: 334 120 800
Fragments arranged by size:
800 334 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1080 1112 1148 1180 1254
Size: 1080 32 36 32 74
Fragments arranged by size:
1080 74 36 32 32
HincII GTy'rAC
Cuts at: 0 11 1249 1254
Size: 11 1238 5
Fragments arranged by size:
1238 11 5
MslI CAynn'nnrTG
Cuts at: 0 584 1254
Size: 584 670
MspA1I CmG'CkG
Cuts at: 0 1029 1177 1254
Size: 1029 148 77
Fragments arranged by size:
1029 148 77
NcoI C'CATG_G
Cuts at: 0 224 1254
Size: 224 1030
NspI r_CATG'y
Cuts at: 0 129 1192 1254
Size: 129 1063 62
Fragments arranged by size:
1063 129 62
PasI CC'CwG_GG
Cuts at: 0 481 948 1072 1254
Size: 481 467 124 182
Fragments arranged by size:
481 467 182 124
PciI A'CATG_T
Cuts at: 0 125 1254
Size: 125 1129
PflMI CCAn_nnn'nTGG
Cuts at: 0 225 1254
Size: 225 1029
PfoI T'CCnGG_A
Cuts at: 0 462 1254
Size: 462 792
PvuII CAG'CTG
Cuts at: 0 1029 1177 1254
Size: 1029 148 77
Fragments arranged by size:
1029 148 77
SacI G_AGCT'C
Cuts at: 0 450 884 1254
Size: 450 434 370
Fragments arranged by size:
450 434 370
SexAI A'CCwGG_T
Cuts at: 0 402 1254
Size: 402 852
SfcI C'TryA_G
Cuts at: 0 62 147 1254
Size: 62 85 1107
Fragments arranged by size:
1107 85 62
SmlI C'TyrA_G
Cuts at: 0 137 905 1254
Size: 137 768 349
Fragments arranged by size:
768 349 137
SspI AAT'ATT
Cuts at: 0 628 925 1254
Size: 628 297 329
Fragments arranged by size:
628 329 297
StuI AGG'CCT
Cuts at: 0 68 297 1254
Size: 68 229 957
Fragments arranged by size:
957 229 68
StyI C'CwwG_G
Cuts at: 0 224 563 569 866 1254
Size: 224 339 6 297 388
Fragments arranged by size:
388 339 297 224 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 499 1254
Size: 499 755
TatI w'GTAC_w
Cuts at: 0 711 1254
Size: 711 543
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1145 1177 1254
Size: 1145 32 77
Fragments arranged by size:
1145 77 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 378 864 1254
Size: 378 486 390
Fragments arranged by size:
486 390 378
Enzymes that do cut:
AcuI AflIII ApaLI ApoI AseI BanI BanII BbsI
BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI BstXI
BstYI BtgI DraIII EarI EcoICRI Eco57MI EcoNI EcoRI
FalI Hin4I HincII MslI MspA1I NcoI NspI PasI
PciI PflMI PfoI PvuII SacI SexAI SfcI SmlI
SspI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EciI EcoO109I EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I
XbaI XhoI XmaI XmnI ZraI