Restriction map for
pFN21AE6313
(Linear) (Six-base) MAPSORT of: KIEE6313.seq Check: 2946 from: 1 to: 1245
KIEE6313.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AccI GT'mk_AC
Cuts at: 0 266 1245
Size: 266 979
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1156 1222 1245
Size: 1156 66 23
Fragments arranged by size:
1156 66 23
AflIII A'CryG_T
Cuts at: 0 380 1245
Size: 380 865
ApoI r'AATT_y
Cuts at: 0 139 430 1245
Size: 139 291 815
Fragments arranged by size:
815 291 139
AscI GG'CGCG_CC
Cuts at: 0 31 1245
Size: 31 1214
AvaI C'yCGr_G
Cuts at: 0 170 884 1245
Size: 170 714 361
Fragments arranged by size:
714 361 170
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 302 335 1245
Size: 302 33 910
Fragments arranged by size:
910 302 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 752 1245
Size: 752 493
BbvCI CC'TCA_GC
Cuts at: 0 679 1245
Size: 679 566
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 733 767 1245
Size: 733 34 478
Fragments arranged by size:
733 478 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1208 1245
Size: 1208 37
BfrBI ATG'CAT
Cuts at: 0 650 1245
Size: 650 595
BglI GCCn_nnn'nGGC
Cuts at: 0 50 638 1245
Size: 50 588 607
Fragments arranged by size:
607 588 50
BmgBI CAC'GTC
Cuts at: 0 304 1245
Size: 304 941
BmrI ACTGGGnnnn_n'
Cuts at: 0 214 1245
Size: 214 1031
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 910 1245
Size: 910 335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1155 1245
Size: 1155 90
Bpu10I CC'TnA_GC
Cuts at: 0 679 1245
Size: 679 566
BsaI GGTCTCn'nnnn_
Cuts at: 0 61 881 1245
Size: 61 820 364
Fragments arranged by size:
820 364 61
BsaAI yAC'GTr
Cuts at: 0 1161 1245
Size: 1161 84
BsaBI GATnn'nnATC
Cuts at: 0 1219 1245
Size: 1219 26
BsaWI w'CCGG_w
Cuts at: 0 83 902 1245
Size: 83 819 343
Fragments arranged by size:
819 343 83
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1039 1245
Size: 1039 206
BseYI C'CCAG_C
Cuts at: 0 245 719 1245
Size: 245 474 526
Fragments arranged by size:
526 474 245
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 1245
Size: 330 915
BsiHKAI G_wGCw'C
Cuts at: 0 1054 1245
Size: 1054 191
BsmI GAATG_Cn'
Cuts at: 0 1087 1245
Size: 1087 158
Bsp1286I G_dGCh'C
Cuts at: 0 1054 1245
Size: 1054 191
BspEI T'CCGG_A
Cuts at: 0 902 1245
Size: 902 343
BsrBI CCG'CTC
Cuts at: 0 11 444 1245
Size: 11 433 801
Fragments arranged by size:
801 433 11
BsrDI GCAATG_nn'
Cuts at: 0 800 1008 1206 1245
Size: 800 208 198 39
Fragments arranged by size:
800 208 198 39
BsrFI r'CCGG_y
Cuts at: 0 17 76 1245
Size: 17 59 1169
Fragments arranged by size:
1169 59 17
BssHII G'CGCG_C
Cuts at: 0 31 1245
Size: 31 1214
BssSI C'ACGA_G
Cuts at: 0 728 1245
Size: 728 517
BstAPI GCAn_nnn'nTGC
Cuts at: 0 647 1161 1245
Size: 647 514 84
Fragments arranged by size:
647 514 84
BstBI TT'CG_AA
Cuts at: 0 743 1245
Size: 743 502
BtgI C'CryG_G
Cuts at: 0 113 452 1055 1245
Size: 113 339 603 190
Fragments arranged by size:
603 339 190 113
BtsI GCAGTG_nn'
Cuts at: 0 1016 1046 1245
Size: 1016 30 199
Fragments arranged by size:
1016 199 30
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 959 994 1245
Size: 959 35 251
Fragments arranged by size:
959 251 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 754 1174 1245
Size: 754 420 71
Fragments arranged by size:
754 420 71
EaeI y'GGCC_r
Cuts at: 0 78 764 926 980 1058 1245
Size: 78 686 162 54 78 187
Fragments arranged by size:
686 187 162 78 78 54
EarI CTCTTCn'nnn_
Cuts at: 0 122 518 1245
Size: 122 396 727
Fragments arranged by size:
727 396 122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1155 1156 1222 1245
Size: 1155 1 66 23
Fragments arranged by size:
1155 66 23 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1237 1245
Size: 1237 8
EcoO109I rG'GnC_Cy
Cuts at: 0 63 631 668 1245
Size: 63 568 37 577
Fragments arranged by size:
577 568 63 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 493 525 722 754 815 847 1245
Size: 493 32 197 32 61 32 398
Fragments arranged by size:
493 398 197 61 32 32 32
MscI TGG'CCA
Cuts at: 0 766 928 1060 1245
Size: 766 162 132 185
Fragments arranged by size:
766 185 162 132
MslI CAynn'nnrTG
Cuts at: 0 601 805 896 1245
Size: 601 204 91 349
Fragments arranged by size:
601 349 204 91
NaeI GCC'GGC
Cuts at: 0 19 1245
Size: 19 1226
NcoI C'CATG_G
Cuts at: 0 113 452 1055 1245
Size: 113 339 603 190
Fragments arranged by size:
603 339 190 113
NgoMIV G'CCGG_C
Cuts at: 0 17 1245
Size: 17 1228
NsiI A_TGCA'T
Cuts at: 0 652 1245
Size: 652 593
NspI r_CATG'y
Cuts at: 0 384 1245
Size: 384 861
PasI CC'CwG_GG
Cuts at: 0 852 1245
Size: 852 393
PciI A'CATG_T
Cuts at: 0 380 1245
Size: 380 865
PflMI CCAn_nnn'nTGG
Cuts at: 0 493 1245
Size: 493 752
PpuMI rG'GwC_Cy
Cuts at: 0 63 668 1245
Size: 63 605 577
Fragments arranged by size:
605 577 63
PstI C_TGCA'G
Cuts at: 0 1022 1245
Size: 1022 223
SanDI GG'GwC_CC
Cuts at: 0 668 1245
Size: 668 577
SapI GCTCTTCn'nnn_
Cuts at: 0 122 1245
Size: 122 1123
ScaI AGT'ACT
Cuts at: 0 940 1245
Size: 940 305
SfcI C'TryA_G
Cuts at: 0 338 1018 1073 1245
Size: 338 680 55 172
Fragments arranged by size:
680 338 172 55
SspI AAT'ATT
Cuts at: 0 837 1245
Size: 837 408
StuI AGG'CCT
Cuts at: 0 568 1105 1245
Size: 568 537 140
Fragments arranged by size:
568 537 140
StyI C'CwwG_G
Cuts at: 0 113 332 452 563 635 1055 1245
Size: 113 219 120 111 72 420 190
Fragments arranged by size:
420 219 190 120 113 111 72
TatI w'GTAC_w
Cuts at: 0 938 1245
Size: 938 307
XbaI T'CTAG_A
Cuts at: 0 1188 1225 1245
Size: 1188 37 20
Fragments arranged by size:
1188 37 20
Enzymes that do cut:
AccI AcuI AflIII ApoI AscI AvaI BaeI BbsI
BbvCI BcgI BciVI BfrBI BglI BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BtgI BtsI CspCI DrdI
EaeI EarI Eco57MI EcoNI EcoO109I Hin4I MscI MslI
NaeI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PpuMI PstI SanDI SapI ScaI SfcI SspI StuI
StyI TatI XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AseI AsiSI
AvrII BamHI BanI BanII BbeI BclI BglII BlpI
Bme1580I BmtI BpuEI BsaHI BsaXI BsiEI BsiWI BsmBI
BspHI BspMI BsrGI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI DraI DraIII EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NarI
NdeI NheI NotI NruI PacI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SwaI TaqII TstI Tth111I XcmI XhoI XmaI XmnI
ZraI