Restriction map for
pFN21AE6312
(Linear) (Six-base) MAPSORT of: KIEE6312.seq Check: 3394 from: 1 to: 942
KIEE6312.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 376 942
Size: 376 566
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 382 810 942
Size: 235 147 428 132
Fragments arranged by size:
428 235 147 132
AleI CACnn'nnGTG
Cuts at: 0 668 942
Size: 668 274
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 781 813 942
Size: 781 32 129
Fragments arranged by size:
781 129 32
AlwNI CAG_nnn'CTG
Cuts at: 0 123 942
Size: 123 819
ApaI G_GGCC'C
Cuts at: 0 871 942
Size: 871 71
AvaI C'yCGr_G
Cuts at: 0 173 515 566 942
Size: 173 342 51 376
Fragments arranged by size:
376 342 173 51
BanI G'GyrC_C
Cuts at: 0 388 397 942
Size: 388 9 545
Fragments arranged by size:
545 388 9
BanII G_rGCy'C
Cuts at: 0 871 942
Size: 871 71
BciVI GTATCCnnnnn_n'
Cuts at: 0 220 942
Size: 220 722
BclI T'GATC_A
Cuts at: 0 680 942
Size: 680 262
BglI GCCn_nnn'nGGC
Cuts at: 0 233 424 942
Size: 233 191 518
Fragments arranged by size:
518 233 191
Bme1580I G_kGCm'C
Cuts at: 0 871 942
Size: 871 71
BmgBI CAC'GTC
Cuts at: 0 792 942
Size: 792 150
BmrI ACTGGGnnnn_n'
Cuts at: 0 254 338 942
Size: 254 84 604
Fragments arranged by size:
604 254 84
BsaBI GATnn'nnATC
Cuts at: 0 508 942
Size: 508 434
BsaWI w'CCGG_w
Cuts at: 0 140 942
Size: 140 802
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 92 122 942
Size: 92 30 820
Fragments arranged by size:
820 92 30
BseYI C'CCAG_C
Cuts at: 0 590 942
Size: 590 352
BsmBI CGTCTCn'nnnn_
Cuts at: 0 931 942
Size: 931 11
Bsp1286I G_dGCh'C
Cuts at: 0 871 942
Size: 871 71
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 376 942
Size: 376 566
BsrBI CCG'CTC
Cuts at: 0 155 942
Size: 155 787
BsrDI GCAATG_nn'
Cuts at: 0 722 942
Size: 722 220
BsrFI r'CCGG_y
Cuts at: 0 25 452 942
Size: 25 427 490
Fragments arranged by size:
490 427 25
BstBI TT'CG_AA
Cuts at: 0 507 942
Size: 507 435
BstXI CCAn_nnnn'nTGG
Cuts at: 0 475 717 942
Size: 475 242 225
Fragments arranged by size:
475 242 225
Bsu36I CC'TnA_GG
Cuts at: 0 229 942
Size: 229 713
BtgI C'CryG_G
Cuts at: 0 474 560 942
Size: 474 86 382
Fragments arranged by size:
474 382 86
BtsI GCAGTG_nn'
Cuts at: 0 698 942
Size: 698 244
DraIII CAC_nnn'GTG
Cuts at: 0 539 942
Size: 539 403
EaeI y'GGCC_r
Cuts at: 0 89 450 942
Size: 89 361 492
Fragments arranged by size:
492 361 89
EarI CTCTTCn'nnn_
Cuts at: 0 515 934 942
Size: 515 419 8
Fragments arranged by size:
515 419 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 382 810 942
Size: 235 147 428 132
Fragments arranged by size:
428 235 147 132
EcoNI CCTnn'n_nnAGG
Cuts at: 0 835 942
Size: 835 107
EcoO109I rG'GnC_Cy
Cuts at: 0 84 868 942
Size: 84 784 74
Fragments arranged by size:
784 84 74
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 445 639 942
Size: 445 194 303
Fragments arranged by size:
445 303 194
HaeII r_GCGC'y
Cuts at: 0 324 474 942
Size: 324 150 468
Fragments arranged by size:
468 324 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 672 704 942
Size: 672 32 238
Fragments arranged by size:
672 238 32
MslI CAynn'nnrTG
Cuts at: 0 668 715 751 942
Size: 668 47 36 191
Fragments arranged by size:
668 191 47 36
MspA1I CmG'CkG
Cuts at: 0 463 686 942
Size: 463 223 256
Fragments arranged by size:
463 256 223
NaeI GCC'GGC
Cuts at: 0 27 454 942
Size: 27 427 488
Fragments arranged by size:
488 427 27
NcoI C'CATG_G
Cuts at: 0 560 942
Size: 560 382
NgoMIV G'CCGG_C
Cuts at: 0 25 452 942
Size: 25 427 490
Fragments arranged by size:
490 427 25
NspI r_CATG'y
Cuts at: 0 750 942
Size: 750 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 123 942
Size: 123 819
PspOMI G'GGCC_C
Cuts at: 0 867 942
Size: 867 75
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 901 933 942
Size: 901 32 9
Fragments arranged by size:
901 32 9
PvuII CAG'CTG
Cuts at: 0 463 942
Size: 463 479
SexAI A'CCwGG_T
Cuts at: 0 128 942
Size: 128 814
SfcI C'TryA_G
Cuts at: 0 104 942
Size: 104 838
SmaI CCC'GGG
Cuts at: 0 517 942
Size: 517 425
SmlI C'TyrA_G
Cuts at: 0 173 942
Size: 173 769
StuI AGG'CCT
Cuts at: 0 227 916 942
Size: 227 689 26
Fragments arranged by size:
689 227 26
StyI C'CwwG_G
Cuts at: 0 294 560 942
Size: 294 266 382
Fragments arranged by size:
382 294 266
TatI w'GTAC_w
Cuts at: 0 179 942
Size: 179 763
XhoI C'TCGA_G
Cuts at: 0 173 942
Size: 173 769
XmaI C'CCGG_G
Cuts at: 0 515 942
Size: 515 427
XmnI GAAnn'nnTTC
Cuts at: 0 841 942
Size: 841 101
Enzymes that do cut:
AarI AcuI AleI AloI AlwNI ApaI AvaI BanI
BanII BciVI BclI BglI Bme1580I BmgBI BmrI BsaBI
BsaWI BsaXI BseYI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstBI BstXI Bsu36I BtgI BtsI DraIII EaeI
EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MslI
MspA1I NaeI NcoI NgoMIV NspI PflMI PspOMI PsrI
PvuII SexAI SfcI SmaI SmlI StuI StyI TatI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BfrBI BglII
BlpI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BspEI
BspHI BsrGI BssHII BssSI BstAPI BstEII BstYI BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NarI NdeI
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI
ZraI