Restriction map for
pFN21AE6311
(Linear) (Six-base) MAPSORT of: KIEE6311.seq Check: 8998 from: 1 to: 1275
KIEE6311.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 499 1275
Size: 499 776
AlwNI CAG_nnn'CTG
Cuts at: 0 993 1155 1188 1275
Size: 993 162 33 87
Fragments arranged by size:
993 162 87 33
ApaI G_GGCC'C
Cuts at: 0 564 1209 1275
Size: 564 645 66
Fragments arranged by size:
645 564 66
ApaLI G'TGCA_C
Cuts at: 0 174 904 1275
Size: 174 730 371
Fragments arranged by size:
730 371 174
AvaI C'yCGr_G
Cuts at: 0 107 837 1171 1275
Size: 107 730 334 104
Fragments arranged by size:
730 334 107 104
BamHI G'GATC_C
Cuts at: 0 548 885 1275
Size: 548 337 390
Fragments arranged by size:
548 390 337
BanI G'GyrC_C
Cuts at: 0 259 664 1275
Size: 259 405 611
Fragments arranged by size:
611 405 259
BanII G_rGCy'C
Cuts at: 0 16 564 1209 1275
Size: 16 548 645 66
Fragments arranged by size:
645 548 66 16
BbeI G_GCGC'C
Cuts at: 0 263 1275
Size: 263 1012
BbsI GAAGACnn'nnnn_
Cuts at: 0 211 1275
Size: 211 1064
BbvCI CC'TCA_GC
Cuts at: 0 63 1275
Size: 63 1212
BclI T'GATC_A
Cuts at: 0 401 1275
Size: 401 874
BglI GCCn_nnn'nGGC
Cuts at: 0 73 388 1275
Size: 73 315 887
Fragments arranged by size:
887 315 73
BglII A'GATC_T
Cuts at: 0 683 1275
Size: 683 592
BlpI GC'TnA_GC
Cuts at: 0 583 1275
Size: 583 692
Bme1580I G_kGCm'C
Cuts at: 0 56 178 564 908 1209 1275
Size: 56 122 386 344 301 66
Fragments arranged by size:
386 344 301 122 66 56
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 436 466 535 1120 1177 1275
Size: 154 282 30 69 585 57 98
Fragments arranged by size:
585 282 154 98 69 57 30
Bpu10I CC'TnA_GC
Cuts at: 0 63 1275
Size: 63 1212
BsaBI GATnn'nnATC
Cuts at: 0 799 1118 1275
Size: 799 319 157
Fragments arranged by size:
799 319 157
BsaHI Gr'CG_yC
Cuts at: 0 4 260 1275
Size: 4 256 1015
Fragments arranged by size:
1015 256 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 138 145 168 1275
Size: 115 23 7 23 1107
Fragments arranged by size:
1107 115 23 23 7
BseYI C'CCAG_C
Cuts at: 0 336 523 698 1224 1275
Size: 336 187 175 526 51
Fragments arranged by size:
526 336 187 175 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 524 1275
Size: 139 385 751
Fragments arranged by size:
751 385 139
BsiHKAI G_wGCw'C
Cuts at: 0 178 422 908 1014 1275
Size: 178 244 486 106 261
Fragments arranged by size:
486 261 244 178 106
Bsp1286I G_dGCh'C
Cuts at: 0 16 56 178 422 564 908 1014 1209
Size: 16 40 122 244 142 344 106 195
Cuts at: 1209 1275
Size: 66
Fragments arranged by size:
344 244 195 142 122 106 66 40
16
BsrFI r'CCGG_y
Cuts at: 0 164 1195 1275
Size: 164 1031 80
Fragments arranged by size:
1031 164 80
BstAPI GCAn_nnn'nTGC
Cuts at: 0 708 1275
Size: 708 567
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1217 1275
Size: 1217 58
BstYI r'GATC_y
Cuts at: 0 548 683 885 1152 1275
Size: 548 135 202 267 123
Fragments arranged by size:
548 267 202 135 123
BtgI C'CryG_G
Cuts at: 0 854 1077 1275
Size: 854 223 198
Fragments arranged by size:
854 223 198
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 801 836 1275
Size: 801 35 439
Fragments arranged by size:
801 439 35
DraIII CAC_nnn'GTG
Cuts at: 0 216 1275
Size: 216 1059
DrdI GACnn_nn'nnGTC
Cuts at: 0 43 1275
Size: 43 1232
EaeI y'GGCC_r
Cuts at: 0 857 1193 1275
Size: 857 336 82
Fragments arranged by size:
857 336 82
EarI CTCTTCn'nnn_
Cuts at: 0 1240 1246 1275
Size: 1240 6 29
Fragments arranged by size:
1240 29 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 436 466 535 1120 1177 1275
Size: 154 282 30 69 585 57 98
Fragments arranged by size:
585 282 154 98 69 57 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 880 1237 1275
Size: 880 357 38
Fragments arranged by size:
880 357 38
EcoO109I rG'GnC_Cy
Cuts at: 0 560 981 1206 1275
Size: 560 421 225 69
Fragments arranged by size:
560 421 225 69
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 750 1275
Size: 750 525
FspI TGC'GCA
Cuts at: 0 712 1275
Size: 712 563
FspAI rTGC'GCAy
Cuts at: 0 712 1275
Size: 712 563
HaeII r_GCGC'y
Cuts at: 0 263 1275
Size: 263 1012
KasI G'GCGC_C
Cuts at: 0 259 1275
Size: 259 1016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 1275
Size: 599 676
MslI CAynn'nnrTG
Cuts at: 0 187 1275
Size: 187 1088
MspA1I CmG'CkG
Cuts at: 0 523 582 1275
Size: 523 59 693
Fragments arranged by size:
693 523 59
NaeI GCC'GGC
Cuts at: 0 166 1197 1275
Size: 166 1031 78
Fragments arranged by size:
1031 166 78
NarI GG'CG_CC
Cuts at: 0 260 1275
Size: 260 1015
NgoMIV G'CCGG_C
Cuts at: 0 164 1195 1275
Size: 164 1031 80
Fragments arranged by size:
1031 164 80
NspI r_CATG'y
Cuts at: 0 400 1275
Size: 400 875
PasI CC'CwG_GG
Cuts at: 0 1095 1275
Size: 1095 180
PflMI CCAn_nnn'nTGG
Cuts at: 0 647 1275
Size: 647 628
PfoI T'CCnGG_A
Cuts at: 0 551 1275
Size: 551 724
PspOMI G'GGCC_C
Cuts at: 0 560 1205 1275
Size: 560 645 70
Fragments arranged by size:
645 560 70
PspXI vC'TCGA_Gb
Cuts at: 0 1171 1275
Size: 1171 104
PvuII CAG'CTG
Cuts at: 0 523 582 1275
Size: 523 59 693
Fragments arranged by size:
693 523 59
RsrII CG'GwC_CG
Cuts at: 0 99 1275
Size: 99 1176
SfoI GGC'GCC
Cuts at: 0 261 1275
Size: 261 1014
SmaI CCC'GGG
Cuts at: 0 109 1275
Size: 109 1166
SmlI C'TyrA_G
Cuts at: 0 1171 1275
Size: 1171 104
StuI AGG'CCT
Cuts at: 0 391 1169 1243 1275
Size: 391 778 74 32
Fragments arranged by size:
778 391 74 32
StyI C'CwwG_G
Cuts at: 0 641 725 1275
Size: 641 84 550
Fragments arranged by size:
641 550 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 116 498 1275
Size: 116 382 777
Fragments arranged by size:
777 382 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 138 170 533 565 1275
Size: 138 32 363 32 710
Fragments arranged by size:
710 363 138 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 586 861 1275
Size: 586 275 414
Fragments arranged by size:
586 414 275
XhoI C'TCGA_G
Cuts at: 0 1171 1275
Size: 1171 104
XmaI C'CCGG_G
Cuts at: 0 107 1275
Size: 107 1168
Enzymes that do cut:
AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BclI BglI BglII BlpI Bme1580I
BpmI Bpu10I BsaBI BsaHI BsaXI BseYI BsgI BsiHKAI
Bsp1286I BsrFI BstAPI BstXI BstYI BtgI CspCI DraIII
DrdI EaeI EarI Eco57MI EcoNI EcoO109I FalI FspI
FspAI HaeII KasI MmeI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PflMI PfoI PspOMI PspXI PvuII
RsrII SfoI SmaI SmlI StuI StyI TaqII TstI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApoI AscI AseI
AsiSI AvrII BaeI BcgI BciVI BfrBI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaWI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI DraI EagI EciI EcoICRI EcoRI EcoRV
FseI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MscI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XmnI
ZraI