Restriction map for
pFN21AE6309
(Linear) (Six-base) MAPSORT of: KIEE6309.seq Check: 2626 from: 1 to: 1116
KIEE6309.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1001 1116
Size: 1001 115
AgeI A'CCGG_T
Cuts at: 0 85 1116
Size: 85 1031
AlwNI CAG_nnn'CTG
Cuts at: 0 272 1026 1116
Size: 272 754 90
Fragments arranged by size:
754 272 90
ApaLI G'TGCA_C
Cuts at: 0 1075 1116
Size: 1075 41
ApoI r'AATT_y
Cuts at: 0 344 847 1116
Size: 344 503 269
Fragments arranged by size:
503 344 269
AseI AT'TA_AT
Cuts at: 0 290 1116
Size: 290 826
AvaI C'yCGr_G
Cuts at: 0 32 1040 1116
Size: 32 1008 76
Fragments arranged by size:
1008 76 32
BanII G_rGCy'C
Cuts at: 0 40 330 1094 1116
Size: 40 290 764 22
Fragments arranged by size:
764 290 40 22
BglII A'GATC_T
Cuts at: 0 311 1116
Size: 311 805
Bme1580I G_kGCm'C
Cuts at: 0 1079 1116
Size: 1079 37
BmgBI CAC'GTC
Cuts at: 0 339 1086 1116
Size: 339 747 30
Fragments arranged by size:
747 339 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 833 1116
Size: 833 283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1116
Size: 151 965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 946 1116
Size: 946 170
BsaI GGTCTCn'nnnn_
Cuts at: 0 499 925 1116
Size: 499 426 191
Fragments arranged by size:
499 426 191
BsaAI yAC'GTr
Cuts at: 0 948 1116
Size: 948 168
BsaWI w'CCGG_w
Cuts at: 0 85 1116
Size: 85 1031
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 760 790 1116
Size: 760 30 326
Fragments arranged by size:
760 326 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 656 1116
Size: 656 460
BseYI C'CCAG_C
Cuts at: 0 25 1116
Size: 25 1091
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 990 1116
Size: 505 485 126
Fragments arranged by size:
505 485 126
BsiHKAI G_wGCw'C
Cuts at: 0 1079 1094 1116
Size: 1079 15 22
Fragments arranged by size:
1079 22 15
BsmI GAATG_Cn'
Cuts at: 0 611 1116
Size: 611 505
Bsp1286I G_dGCh'C
Cuts at: 0 40 330 1079 1094 1116
Size: 40 290 749 15 22
Fragments arranged by size:
749 290 40 22 15
BspHI T'CATG_A
Cuts at: 0 624 1094 1116
Size: 624 470 22
Fragments arranged by size:
624 470 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 280 1116
Size: 280 836
BsrFI r'CCGG_y
Cuts at: 0 12 85 671 934 971 1116
Size: 12 73 586 263 37 145
Fragments arranged by size:
586 263 145 73 37 12
BstEII G'GTnAC_C
Cuts at: 0 527 1116
Size: 527 589
BstXI CCAn_nnnn'nTGG
Cuts at: 0 72 1116
Size: 72 1044
BstYI r'GATC_y
Cuts at: 0 311 957 1116
Size: 311 646 159
Fragments arranged by size:
646 311 159
BtgI C'CryG_G
Cuts at: 0 150 1116
Size: 150 966
BtsI GCAGTG_nn'
Cuts at: 0 368 608 1116
Size: 368 240 508
Fragments arranged by size:
508 368 240
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 965 1000 1116
Size: 965 35 116
Fragments arranged by size:
965 116 35
EaeI y'GGCC_r
Cuts at: 0 973 1116
Size: 973 143
EarI CTCTTCn'nnn_
Cuts at: 0 622 633 1116
Size: 622 11 483
Fragments arranged by size:
622 483 11
EcoICRI GAG'CTC
Cuts at: 0 1092 1116
Size: 1092 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1001 1116
Size: 151 850 115
Fragments arranged by size:
850 151 115
HaeII r_GCGC'y
Cuts at: 0 32 79 1116
Size: 32 47 1037
Fragments arranged by size:
1037 47 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 942 974 1116
Size: 942 32 142
Fragments arranged by size:
942 142 32
HincII GTy'rAC
Cuts at: 0 381 1116
Size: 381 735
NcoI C'CATG_G
Cuts at: 0 150 1116
Size: 150 966
PflMI CCAn_nnn'nTGG
Cuts at: 0 890 1116
Size: 890 226
PfoI T'CCnGG_A
Cuts at: 0 842 1116
Size: 842 274
PmlI CAC'GTG
Cuts at: 0 948 1116
Size: 948 168
PstI C_TGCA'G
Cuts at: 0 998 1116
Size: 998 118
SacI G_AGCT'C
Cuts at: 0 1094 1116
Size: 1094 22
ScaI AGT'ACT
Cuts at: 0 967 1116
Size: 967 149
SfcI C'TryA_G
Cuts at: 0 994 1116
Size: 994 122
SmaI CCC'GGG
Cuts at: 0 34 1042 1116
Size: 34 1008 74
Fragments arranged by size:
1008 74 34
SmlI C'TyrA_G
Cuts at: 0 961 1116
Size: 961 155
StyI C'CwwG_G
Cuts at: 0 150 321 693 755 1116
Size: 150 171 372 62 361
Fragments arranged by size:
372 361 171 150 62
TatI w'GTAC_w
Cuts at: 0 365 965 1116
Size: 365 600 151
Fragments arranged by size:
600 365 151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 760 792 1116
Size: 760 32 324
Fragments arranged by size:
760 324 32
XbaI T'CTAG_A
Cuts at: 0 405 1116
Size: 405 711
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 762 1116
Size: 762 354
XmaI C'CCGG_G
Cuts at: 0 32 1040 1116
Size: 32 1008 76
Fragments arranged by size:
1008 76 32
XmnI GAAnn'nnTTC
Cuts at: 0 862 1116
Size: 862 254
Enzymes that do cut:
AcuI AgeI AlwNI ApaLI ApoI AseI AvaI BanII
BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrFI BstEII BstXI BstYI BtgI BtsI
CspCI EaeI EarI EcoICRI Eco57MI HaeII Hin4I HincII
NcoI PflMI PfoI PmlI PstI SacI ScaI SfcI
SmaI SmlI StyI TatI TstI XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaI AscI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmtI BplI Bpu10I BsaBI
BsaHI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI
DraI DraIII DrdI EagI EciI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
Tth111I XhoI ZraI