Restriction map for
pFN21AE6307
(Linear) (Six-base) MAPSORT of: KIEE6307.seq Check: 7318 from: 1 to: 942
KIEE6307.orf
With 182 enzymes: SgfI *
July 1, 2014 11:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 272 481 942
Size: 131 141 209 461
Fragments arranged by size:
461 209 141 131
AflIII A'CryG_T
Cuts at: 0 514 942
Size: 514 428
ApaI G_GGCC'C
Cuts at: 0 261 942
Size: 261 681
ApaLI G'TGCA_C
Cuts at: 0 155 942
Size: 155 787
ApoI r'AATT_y
Cuts at: 0 535 591 772 942
Size: 535 56 181 170
Fragments arranged by size:
535 181 170 56
BamHI G'GATC_C
Cuts at: 0 528 726 942
Size: 528 198 216
Fragments arranged by size:
528 216 198
BanI G'GyrC_C
Cuts at: 0 3 334 942
Size: 3 331 608
Fragments arranged by size:
608 331 3
BanII G_rGCy'C
Cuts at: 0 261 311 942
Size: 261 50 631
Fragments arranged by size:
631 261 50
BbeI G_GCGC'C
Cuts at: 0 7 942
Size: 7 935
BfrBI ATG'CAT
Cuts at: 0 924 942
Size: 924 18
Bme1580I G_kGCm'C
Cuts at: 0 159 261 942
Size: 159 102 681
Fragments arranged by size:
681 159 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 942
Size: 247 695
Bpu10I CC'TnA_GC
Cuts at: 0 68 942
Size: 68 874
BsaAI yAC'GTr
Cuts at: 0 344 942
Size: 344 598
BsaBI GATnn'nnATC
Cuts at: 0 725 942
Size: 725 217
BsaHI Gr'CG_yC
Cuts at: 0 4 942
Size: 4 938
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 322 352 942
Size: 322 30 590
Fragments arranged by size:
590 322 30
BseYI C'CCAG_C
Cuts at: 0 364 942
Size: 364 578
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 942
Size: 476 466
BsiHKAI G_wGCw'C
Cuts at: 0 159 942
Size: 159 783
Bsp1286I G_dGCh'C
Cuts at: 0 159 261 311 942
Size: 159 102 50 631
Fragments arranged by size:
631 159 102 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3 409 942
Size: 3 406 533
Fragments arranged by size:
533 406 3
BsrBI CCG'CTC
Cuts at: 0 305 942
Size: 305 637
BssSI C'ACGA_G
Cuts at: 0 543 942
Size: 543 399
BstAPI GCAn_nnn'nTGC
Cuts at: 0 838 942
Size: 838 104
BstXI CCAn_nnnn'nTGG
Cuts at: 0 198 942
Size: 198 744
BstYI r'GATC_y
Cuts at: 0 528 720 726 942
Size: 528 192 6 216
Fragments arranged by size:
528 216 192 6
BtgI C'CryG_G
Cuts at: 0 41 942
Size: 41 901
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 311 942
Size: 311 631
BtsI GCAGTG_nn'
Cuts at: 0 331 359 942
Size: 331 28 583
Fragments arranged by size:
583 331 28
EaeI y'GGCC_r
Cuts at: 0 101 942
Size: 101 841
EarI CTCTTCn'nnn_
Cuts at: 0 234 868 942
Size: 234 634 74
Fragments arranged by size:
634 234 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 247 272 481 942
Size: 131 116 25 209 461
Fragments arranged by size:
461 209 131 116 25
EcoO109I rG'GnC_Cy
Cuts at: 0 15 257 258 942
Size: 15 242 1 684
Fragments arranged by size:
684 242 15 1
EcoRI G'AATT_C
Cuts at: 0 772 942
Size: 772 170
HaeII r_GCGC'y
Cuts at: 0 7 942
Size: 7 935
KasI G'GCGC_C
Cuts at: 0 3 942
Size: 3 939
MluI A'CGCG_T
Cuts at: 0 514 942
Size: 514 428
MspA1I CmG'CkG
Cuts at: 0 43 942
Size: 43 899
NarI GG'CG_CC
Cuts at: 0 4 942
Size: 4 938
NsiI A_TGCA'T
Cuts at: 0 926 942
Size: 926 16
PflMI CCAn_nnn'nTGG
Cuts at: 0 347 942
Size: 347 595
PmlI CAC'GTG
Cuts at: 0 344 942
Size: 344 598
PpuMI rG'GwC_Cy
Cuts at: 0 15 942
Size: 15 927
PspOMI G'GGCC_C
Cuts at: 0 257 942
Size: 257 685
SacII CC_GC'GG
Cuts at: 0 44 942
Size: 44 898
SapI GCTCTTCn'nnn_
Cuts at: 0 234 942
Size: 234 708
SfoI GGC'GCC
Cuts at: 0 5 942
Size: 5 937
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 904 942
Size: 904 38
TatI w'GTAC_w
Cuts at: 0 142 942
Size: 142 800
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 836 868 942
Size: 836 32 74
Fragments arranged by size:
836 74 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 271 942
Size: 271 671
Enzymes that do cut:
AcuI AflIII ApaI ApaLI ApoI BamHI BanI BanII
BbeI BfrBI Bme1580I BpmI Bpu10I BsaAI BsaBI BsaHI
BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BssSI
BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EarI
Eco57MI EcoO109I EcoRI HaeII KasI MluI MspA1I NarI
NsiI PflMI PmlI PpuMI PspOMI SacII SapI SfoI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI AscI AseI AsiSI
AvaI AvrII BaeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI
BsaI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MmeI MscI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PfoI PmeI PpiI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI