Restriction map for
pFN21AE6304
(Linear) (Six-base) MAPSORT of: KIEE6304.seq Check: 3971 from: 1 to: 1329
KIEE6304.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1262 1291 1329
Size: 1262 29 38
Fragments arranged by size:
1262 38 29
AccI GT'mk_AC
Cuts at: 0 808 1329
Size: 808 521
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 1329
Size: 248 1081
AlwNI CAG_nnn'CTG
Cuts at: 0 1114 1329
Size: 1114 215
ApaI G_GGCC'C
Cuts at: 0 547 1319 1329
Size: 547 772 10
Fragments arranged by size:
772 547 10
ApoI r'AATT_y
Cuts at: 0 464 1329
Size: 464 865
AvaI C'yCGr_G
Cuts at: 0 1013 1047 1329
Size: 1013 34 282
Fragments arranged by size:
1013 282 34
BanI G'GyrC_C
Cuts at: 0 633 1250 1329
Size: 633 617 79
Fragments arranged by size:
633 617 79
BanII G_rGCy'C
Cuts at: 0 547 783 791 1222 1319 1329
Size: 547 236 8 431 97 10
Fragments arranged by size:
547 431 236 97 10 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 389 1010 1329
Size: 389 621 319
Fragments arranged by size:
621 389 319
Bme1580I G_kGCm'C
Cuts at: 0 547 1319 1329
Size: 547 772 10
Fragments arranged by size:
772 547 10
BmtI G_CTAG'C
Cuts at: 0 706 1329
Size: 706 623
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 387 967 1329
Size: 387 580 362
Fragments arranged by size:
580 387 362
Bpu10I CC'TnA_GC
Cuts at: 0 985 1329
Size: 985 344
BsaI GGTCTCn'nnnn_
Cuts at: 0 291 1329
Size: 291 1038
BsaAI yAC'GTr
Cuts at: 0 1200 1329
Size: 1200 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 7 37 44 74 854 884 1329
Size: 7 30 7 30 780 30 445
Fragments arranged by size:
780 445 30 30 30 7 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 15 1329
Size: 15 1314
BseYI C'CCAG_C
Cuts at: 0 313 989 1324 1329
Size: 313 676 335 5
Fragments arranged by size:
676 335 313 5
BsiEI CG_ry'CG
Cuts at: 0 1294 1329
Size: 1294 35
BsiHKAI G_wGCw'C
Cuts at: 0 1222 1329
Size: 1222 107
Bsp1286I G_dGCh'C
Cuts at: 0 547 783 791 1222 1319 1329
Size: 547 236 8 431 97 10
Fragments arranged by size:
547 431 236 97 10 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 820 1262 1291 1329
Size: 820 442 29 38
Fragments arranged by size:
820 442 38 29
BsrBI CCG'CTC
Cuts at: 0 1298 1329
Size: 1298 31
BsrDI GCAATG_nn'
Cuts at: 0 842 1329
Size: 842 487
BsrFI r'CCGG_y
Cuts at: 0 1058 1329
Size: 1058 271
BssSI C'ACGA_G
Cuts at: 0 1093 1329
Size: 1093 236
BstAPI GCAn_nnn'nTGC
Cuts at: 0 601 1329
Size: 601 728
BstEII G'GTnAC_C
Cuts at: 0 1038 1329
Size: 1038 291
BstZ17I GTA'TAC
Cuts at: 0 809 1329
Size: 809 520
BtgI C'CryG_G
Cuts at: 0 908 1329
Size: 908 421
BtsI GCAGTG_nn'
Cuts at: 0 142 515 1077 1329
Size: 142 373 562 252
Fragments arranged by size:
562 373 252 142
EaeI y'GGCC_r
Cuts at: 0 2 905 1291 1329
Size: 2 903 386 38
Fragments arranged by size:
903 386 38 2
EagI C'GGCC_G
Cuts at: 0 1291 1329
Size: 1291 38
EarI CTCTTCn'nnn_
Cuts at: 0 796 1329
Size: 796 533
EcoICRI GAG'CTC
Cuts at: 0 1220 1329
Size: 1220 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 1329
Size: 248 1081
EcoO109I rG'GnC_Cy
Cuts at: 0 544 993 1315 1316 1329
Size: 544 449 322 1 13
Fragments arranged by size:
544 449 322 13 1
HaeII r_GCGC'y
Cuts at: 0 1072 1329
Size: 1072 257
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 44 76 285 317 1329
Size: 44 32 209 32 1012
Fragments arranged by size:
1012 209 44 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 840 1329
Size: 840 489
MspA1I CmG'CkG
Cuts at: 0 189 523 675 904 1260 1329
Size: 189 334 152 229 356 69
Fragments arranged by size:
356 334 229 189 152 69
NaeI GCC'GGC
Cuts at: 0 1060 1329
Size: 1060 269
NgoMIV G'CCGG_C
Cuts at: 0 1058 1329
Size: 1058 271
NheI G'CTAG_C
Cuts at: 0 702 1329
Size: 702 627
NspI r_CATG'y
Cuts at: 0 177 1329
Size: 177 1152
PasI CC'CwG_GG
Cuts at: 0 783 1329
Size: 783 546
PflMI CCAn_nnn'nTGG
Cuts at: 0 854 1083 1329
Size: 854 229 246
Fragments arranged by size:
854 246 229
PfoI T'CCnGG_A
Cuts at: 0 735 1329
Size: 735 594
PspOMI G'GGCC_C
Cuts at: 0 543 1315 1329
Size: 543 772 14
Fragments arranged by size:
772 543 14
PstI C_TGCA'G
Cuts at: 0 522 1243 1329
Size: 522 721 86
Fragments arranged by size:
721 522 86
PvuII CAG'CTG
Cuts at: 0 189 523 1329
Size: 189 334 806
Fragments arranged by size:
806 334 189
SacI G_AGCT'C
Cuts at: 0 1222 1329
Size: 1222 107
SapI GCTCTTCn'nnn_
Cuts at: 0 796 1329
Size: 796 533
SfcI C'TryA_G
Cuts at: 0 518 1239 1329
Size: 518 721 90
Fragments arranged by size:
721 518 90
SmaI CCC'GGG
Cuts at: 0 1015 1049 1329
Size: 1015 34 280
Fragments arranged by size:
1015 280 34
StuI AGG'CCT
Cuts at: 0 590 1329
Size: 590 739
StyI C'CwwG_G
Cuts at: 0 755 1329
Size: 755 574
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1026 1057 1329
Size: 1026 31 272
Fragments arranged by size:
1026 272 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 7 39 409 441 1329
Size: 7 32 370 32 888
Fragments arranged by size:
888 370 32 32 7
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 753 1329
Size: 753 576
XmaI C'CCGG_G
Cuts at: 0 1013 1047 1329
Size: 1013 34 282
Fragments arranged by size:
1013 282 34
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaI ApoI AvaI BanI
BanII BbsI Bme1580I BmtI BplI Bpu10I BsaI BsaAI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstEII BstZ17I BtgI BtsI
EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I
MmeI MspA1I NaeI NgoMIV NheI NspI PasI PflMI
PfoI PspOMI PstI PvuII SacI SapI SfcI SmaI
StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BpmI BpuEI BsaBI
BsaHI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI
BsrGI BssHII BstBI BstXI BstYI Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MscI MslI NarI NcoI NdeI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TatI Tth111I XbaI XhoI XmnI ZraI