Restriction map for
pFN21AE6302
(Linear) (Six-base) MAPSORT of: KIEE6302.seq Check: 9561 from: 1 to: 933
KIEE6302.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
Acc65I G'GTAC_C
Cuts at: 0 188 623 933
Size: 188 435 310
Fragments arranged by size:
435 310 188
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 586 820 933
Size: 550 36 234 113
Fragments arranged by size:
550 234 113 36
AfeI AGC'GCT
Cuts at: 0 172 933
Size: 172 761
AflIII A'CryG_T
Cuts at: 0 364 773 933
Size: 364 409 160
Fragments arranged by size:
409 364 160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 349 933
Size: 349 584
AlwNI CAG_nnn'CTG
Cuts at: 0 559 750 798 933
Size: 559 191 48 135
Fragments arranged by size:
559 191 135 48
AvaI C'yCGr_G
Cuts at: 0 72 933
Size: 72 861
BanI G'GyrC_C
Cuts at: 0 188 623 933
Size: 188 435 310
Fragments arranged by size:
435 310 188
BanII G_rGCy'C
Cuts at: 0 577 815 933
Size: 577 238 118
Fragments arranged by size:
577 238 118
BbsI GAAGACnn'nnnn_
Cuts at: 0 154 666 931 933
Size: 154 512 265 2
Fragments arranged by size:
512 265 154 2
BbvCI CC'TCA_GC
Cuts at: 0 322 933
Size: 322 611
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 205 239 933
Size: 205 34 694
Fragments arranged by size:
694 205 34
BclI T'GATC_A
Cuts at: 0 662 933
Size: 662 271
BglII A'GATC_T
Cuts at: 0 116 933
Size: 116 817
Bme1580I G_kGCm'C
Cuts at: 0 424 835 933
Size: 424 411 98
Fragments arranged by size:
424 411 98
BmrI ACTGGGnnnn_n'
Cuts at: 0 677 933
Size: 677 256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 933
Size: 772 161
Bpu10I CC'TnA_GC
Cuts at: 0 259 322 933
Size: 259 63 611
Fragments arranged by size:
611 259 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 933
Size: 799 134
BsaI GGTCTCn'nnnn_
Cuts at: 0 424 933
Size: 424 509
BsaAI yAC'GTr
Cuts at: 0 144 776 933
Size: 144 632 157
Fragments arranged by size:
632 157 144
BsaBI GATnn'nnATC
Cuts at: 0 121 933
Size: 121 812
BsaHI Gr'CG_yC
Cuts at: 0 236 933
Size: 236 697
BsaWI w'CCGG_w
Cuts at: 0 767 933
Size: 767 166
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 448 478 933
Size: 448 30 455
Fragments arranged by size:
455 448 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 707 933
Size: 707 226
BsiEI CG_ry'CG
Cuts at: 0 35 296 933
Size: 35 261 637
Fragments arranged by size:
637 261 35
BsmI GAATG_Cn'
Cuts at: 0 820 933
Size: 820 113
Bsp1286I G_dGCh'C
Cuts at: 0 424 577 815 835 933
Size: 424 153 238 20 98
Fragments arranged by size:
424 238 153 98 20
BspHI T'CATG_A
Cuts at: 0 602 933
Size: 602 331
BssHII G'CGCG_C
Cuts at: 0 21 933
Size: 21 912
BstYI r'GATC_y
Cuts at: 0 116 794 933
Size: 116 678 139
Fragments arranged by size:
678 139 116
BtgI C'CryG_G
Cuts at: 0 184 231 252 407 425 495 933
Size: 184 47 21 155 18 70 438
Fragments arranged by size:
438 184 155 70 47 21 18
EaeI y'GGCC_r
Cuts at: 0 32 293 398 869 933
Size: 32 261 105 471 64
Fragments arranged by size:
471 261 105 64 32
EagI C'GGCC_G
Cuts at: 0 32 293 933
Size: 32 261 640
Fragments arranged by size:
640 261 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 586 772 820 933
Size: 550 36 186 48 113
Fragments arranged by size:
550 186 113 48 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 568 802 933
Size: 568 234 131
Fragments arranged by size:
568 234 131
EcoO109I rG'GnC_Cy
Cuts at: 0 546 747 933
Size: 546 201 186
Fragments arranged by size:
546 201 186
FspI TGC'GCA
Cuts at: 0 616 933
Size: 616 317
HaeII r_GCGC'y
Cuts at: 0 174 392 933
Size: 174 218 541
Fragments arranged by size:
541 218 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 157 189 448 480 933
Size: 157 32 259 32 453
Fragments arranged by size:
453 259 157 32 32
KpnI G_GTAC'C
Cuts at: 0 192 627 933
Size: 192 435 306
Fragments arranged by size:
435 306 192
MluI A'CGCG_T
Cuts at: 0 364 933
Size: 364 569
MscI TGG'CCA
Cuts at: 0 871 933
Size: 871 62
MslI CAynn'nnrTG
Cuts at: 0 439 933
Size: 439 494
MspA1I CmG'CkG
Cuts at: 0 156 162 186 254 933
Size: 156 6 24 68 679
Fragments arranged by size:
679 156 68 24 6
NcoI C'CATG_G
Cuts at: 0 231 425 933
Size: 231 194 508
Fragments arranged by size:
508 231 194
NotI GC'GGCC_GC
Cuts at: 0 32 933
Size: 32 901
NspI r_CATG'y
Cuts at: 0 336 933
Size: 336 597
PasI CC'CwG_GG
Cuts at: 0 345 933
Size: 345 588
PfoI T'CCnGG_A
Cuts at: 0 49 933
Size: 49 884
PmlI CAC'GTG
Cuts at: 0 776 933
Size: 776 157
PstI C_TGCA'G
Cuts at: 0 161 933
Size: 161 772
PvuII CAG'CTG
Cuts at: 0 156 162 933
Size: 156 6 771
Fragments arranged by size:
771 156 6
SacII CC_GC'GG
Cuts at: 0 187 255 933
Size: 187 68 678
Fragments arranged by size:
678 187 68
SfcI C'TryA_G
Cuts at: 0 157 933
Size: 157 776
SmaI CCC'GGG
Cuts at: 0 74 933
Size: 74 859
SmlI C'TyrA_G
Cuts at: 0 814 933
Size: 814 119
StyI C'CwwG_G
Cuts at: 0 231 287 425 933
Size: 231 56 138 508
Fragments arranged by size:
508 231 138 56
TatI w'GTAC_w
Cuts at: 0 479 933
Size: 479 454
XmaI C'CCGG_G
Cuts at: 0 72 933
Size: 72 861
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AlfI AlwNI AvaI BanI
BanII BbsI BbvCI BcgI BclI BglII Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BsiEI BsmI Bsp1286I BspHI BssHII BstYI
BtgI EaeI EagI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I KpnI MluI MscI MslI MspA1I NcoI NotI
NspI PasI PfoI PmlI PstI PvuII SacII SfcI
SmaI SmlI StyI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AleI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BfrBI BglI BlpI BmgBI
BmtI BplI BseYI BsgI BsiHKAI BsiWI BsmBI BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EarI EciI EcoICRI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KasI MfeI
MmeI NaeI NarI NdeI NgoMIV NheI NruI NsiI
PacI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmnI ZraI