Restriction map for
pFN21AE6301
(Linear) (Six-base) MAPSORT of: KIEE6301.seq Check: 6452 from: 1 to: 1269
KIEE6301.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AccI GT'mk_AC
Cuts at: 0 896 1269
Size: 896 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 696 748 1269
Size: 475 221 52 521
Fragments arranged by size:
521 475 221 52
AfeI AGC'GCT
Cuts at: 0 256 385 1269
Size: 256 129 884
Fragments arranged by size:
884 256 129
AleI CACnn'nnGTG
Cuts at: 0 1138 1269
Size: 1138 131
AlwNI CAG_nnn'CTG
Cuts at: 0 246 1269
Size: 246 1023
ApaI G_GGCC'C
Cuts at: 0 472 1269
Size: 472 797
ApaLI G'TGCA_C
Cuts at: 0 421 1269
Size: 421 848
ApoI r'AATT_y
Cuts at: 0 963 1269
Size: 963 306
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 505 538 1269
Size: 505 33 731
Fragments arranged by size:
731 505 33
BanII G_rGCy'C
Cuts at: 0 472 1079 1269
Size: 472 607 190
Fragments arranged by size:
607 472 190
BbsI GAAGACnn'nnnn_
Cuts at: 0 151 1269
Size: 151 1118
BbvCI CC'TCA_GC
Cuts at: 0 341 1269
Size: 341 928
BglI GCCn_nnn'nGGC
Cuts at: 0 352 1028 1269
Size: 352 676 241
Fragments arranged by size:
676 352 241
BlpI GC'TnA_GC
Cuts at: 0 1018 1269
Size: 1018 251
Bme1580I G_kGCm'C
Cuts at: 0 425 434 472 1088 1269
Size: 425 9 38 616 181
Fragments arranged by size:
616 425 181 38 9
BmgBI CAC'GTC
Cuts at: 0 1245 1269
Size: 1245 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 237 244 1269
Size: 237 7 1025
Fragments arranged by size:
1025 237 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 42 619 1269
Size: 25 17 577 650
Fragments arranged by size:
650 577 25 17
Bpu10I CC'TnA_GC
Cuts at: 0 341 1269
Size: 341 928
BsaI GGTCTCn'nnnn_
Cuts at: 0 122 496 595 1269
Size: 122 374 99 674
Fragments arranged by size:
674 374 122 99
BsaWI w'CCGG_w
Cuts at: 0 323 1211 1269
Size: 323 888 58
Fragments arranged by size:
888 323 58
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 1171 1186 1269
Size: 28 1143 15 83
Fragments arranged by size:
1143 83 28 15
BseYI C'CCAG_C
Cuts at: 0 824 1223 1269
Size: 824 399 46
Fragments arranged by size:
824 399 46
BsiHKAI G_wGCw'C
Cuts at: 0 425 1269
Size: 425 844
BsmI GAATG_Cn'
Cuts at: 0 549 1269
Size: 549 720
BsmBI CGTCTCn'nnnn_
Cuts at: 0 697 1269
Size: 697 572
Bsp1286I G_dGCh'C
Cuts at: 0 425 434 472 1079 1088 1269
Size: 425 9 38 607 9 181
Fragments arranged by size:
607 425 181 38 9 9
BspEI T'CCGG_A
Cuts at: 0 1211 1269
Size: 1211 58
BspHI T'CATG_A
Cuts at: 0 332 1269
Size: 332 937
BsrBI CCG'CTC
Cuts at: 0 1017 1269
Size: 1017 252
BsrFI r'CCGG_y
Cuts at: 0 287 698 1269
Size: 287 411 571
Fragments arranged by size:
571 411 287
BssSI C'ACGA_G
Cuts at: 0 402 1269
Size: 402 867
BstAPI GCAn_nnn'nTGC
Cuts at: 0 429 651 1269
Size: 429 222 618
Fragments arranged by size:
618 429 222
BstXI CCAn_nnnn'nTGG
Cuts at: 0 193 840 1231 1269
Size: 193 647 391 38
Fragments arranged by size:
647 391 193 38
BtgI C'CryG_G
Cuts at: 0 123 1269
Size: 123 1146
BtsI GCAGTG_nn'
Cuts at: 0 1048 1269
Size: 1048 221
DraIII CAC_nnn'GTG
Cuts at: 0 429 1269
Size: 429 840
EaeI y'GGCC_r
Cuts at: 0 566 1012 1269
Size: 566 446 257
Fragments arranged by size:
566 446 257
EarI CTCTTCn'nnn_
Cuts at: 0 367 634 733 1180 1213 1269
Size: 367 267 99 447 33 56
Fragments arranged by size:
447 367 267 99 56 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 763 1269
Size: 763 506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 42 475 619 696 748 1269
Size: 25 17 433 144 77 52 521
Fragments arranged by size:
521 433 144 77 52 25 17
EcoO109I rG'GnC_Cy
Cuts at: 0 159 243 1005 1269
Size: 159 84 762 264
Fragments arranged by size:
762 264 159 84
FspI TGC'GCA
Cuts at: 0 854 1269
Size: 854 415
HaeII r_GCGC'y
Cuts at: 0 258 387 1269
Size: 258 129 882
Fragments arranged by size:
882 258 129
HincII GTy'rAC
Cuts at: 0 203 1269
Size: 203 1066
HpaI GTT'AAC
Cuts at: 0 203 1269
Size: 203 1066
MscI TGG'CCA
Cuts at: 0 568 1269
Size: 568 701
MslI CAynn'nnrTG
Cuts at: 0 1138 1269
Size: 1138 131
MspA1I CmG'CkG
Cuts at: 0 1043 1113 1269
Size: 1043 70 156
Fragments arranged by size:
1043 156 70
NaeI GCC'GGC
Cuts at: 0 700 1269
Size: 700 569
NcoI C'CATG_G
Cuts at: 0 123 1269
Size: 123 1146
NgoMIV G'CCGG_C
Cuts at: 0 698 1269
Size: 698 571
PasI CC'CwG_GG
Cuts at: 0 186 665 1269
Size: 186 479 604
Fragments arranged by size:
604 479 186
PflMI CCAn_nnn'nTGG
Cuts at: 0 1094 1269
Size: 1094 175
PpuMI rG'GwC_Cy
Cuts at: 0 159 1269
Size: 159 1110
PspOMI G'GGCC_C
Cuts at: 0 468 1269
Size: 468 801
PstI C_TGCA'G
Cuts at: 0 1055 1131 1269
Size: 1055 76 138
Fragments arranged by size:
1055 138 76
PvuII CAG'CTG
Cuts at: 0 1113 1269
Size: 1113 156
RsrII CG'GwC_CG
Cuts at: 0 14 1269
Size: 14 1255
SexAI A'CCwGG_T
Cuts at: 0 62 1269
Size: 62 1207
SfcI C'TryA_G
Cuts at: 0 220 493 1051 1127 1269
Size: 220 273 558 76 142
Fragments arranged by size:
558 273 220 142 76
StyI C'CwwG_G
Cuts at: 0 123 155 206 1088 1151 1269
Size: 123 32 51 882 63 118
Fragments arranged by size:
882 123 118 63 51 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 97 1269
Size: 97 1172
TatI w'GTAC_w
Cuts at: 0 980 1269
Size: 980 289
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 981 1013 1269
Size: 981 32 256
Fragments arranged by size:
981 256 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 955 1249 1269
Size: 955 294 20
Fragments arranged by size:
955 294 20
Enzymes that do cut:
AccI AcuI AfeI AleI AlwNI ApaI ApaLI ApoI
BaeI BanII BbsI BbvCI BglI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaI BsaWI BseRI BseYI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BssSI
BstAPI BstXI BtgI BtsI DraIII EaeI EarI EciI
Eco57MI EcoO109I FspI HaeII HincII HpaI MscI MslI
MspA1I NaeI NcoI NgoMIV PasI PflMI PpuMI PspOMI
PstI PvuII RsrII SexAI SfcI StyI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI AscI AseI AsiSI AvaI AvrII BamHI
BanI BbeI BcgI BciVI BclI BfrBI BglII BmtI
BplI BpuEI BsaAI BsaBI BsaHI BsaXI BsgI BsiEI
BsiWI BspMI BsrDI BsrGI BssHII BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I
HindIII KasI KpnI MfeI MluI MmeI NarI NdeI
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI